Insulin-stimulated translocation of glucose transport systems in the isolated rat adipose cell. Time course, reversal, insulin concentration dependency, and relationship to glucose transport activity

A specific D-glucose-inhibitable ( super(3)H)cytochalasin B-binding assay is used to examine the time course, reversibility, and hormone concentration dependency of an insulin-induced translocation of glucose transport systems from a large intracellular pool, associated with a low density subfraction of the microsomal membranes, to the plasma membrane in isolated rat adipose cells. The number of glucose transport systems in the plasma membrane and glucose transport activity in the intact cell are compared during the time course and reversal of insulin's action. The results demonstrate that insulin increases the number of glucose transport systems in the plasma membrane of the isolated rat adipose cell through a rapid and reversible translocation of glucose transport systems from a specific intracellular membrane pool.