Triiodothyronine Is a High‐Affinity Inhibitor of Amino Acid Transport System L1 in Cultured Astrocytes
: The relationship between the transport of thyroid hormones and that of amino acids was examined by measuring the uptake of amino acids that are characteristic substrates of systems L, A, and N, and the effect of 3,3′,5‐triiodo‐L‐thyronine (T3) on this uptake, in cultured astrocytes. Tryptophan and...
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| Veröffentlicht in: | Journal of neurochemistry 1993-04, Vol.60 (4), p.1407-1413 |
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| creator | Blondeau, Jean‐Paul Beslin, Aline Chantoux, Françoise Francon, Jacques |
| description | : The relationship between the transport of thyroid hormones and that of amino acids was examined by measuring the uptake of amino acids that are characteristic substrates of systems L, A, and N, and the effect of 3,3′,5‐triiodo‐L‐thyronine (T3) on this uptake, in cultured astrocytes. Tryptophan and leucine uptakes were rapid, Na+‐independent, and efficiently inhibited by T3 (half‐inhibition at ∼ 2 μM). Two Na+‐independent L‐like systems (L1 and L2), common to leucine and aromatic amino acids, were characterized kinetically. System L2 had a low affinity for leucine and tryptophan (Km= 0.3–0.9 mM). The high‐affinity system L1 (Km∼ 10 μM for both amino acids) was competitively inhibited by T3 with a Ki of 2–3 μM (close to the T3 transport Km). Several T3 analogues inhibited system L1 and the T3 transport system similarly. Glutamine uptake and α‐(methylamino)isobutyric acid uptake were, respectively, two and 200 times lower than tryptophan and leucine uptakes. T3 had little effect on the uptakes of glutamine and α‐(methylamino)isobutyric acid. The results indicate that the T3 transport system and system L1 are related. |
| doi_str_mv | 10.1111/j.1471-4159.1993.tb03302.x |
| format | Article |
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Tryptophan and leucine uptakes were rapid, Na+‐independent, and efficiently inhibited by T3 (half‐inhibition at ∼ 2 μM). Two Na+‐independent L‐like systems (L1 and L2), common to leucine and aromatic amino acids, were characterized kinetically. System L2 had a low affinity for leucine and tryptophan (Km= 0.3–0.9 mM). The high‐affinity system L1 (Km∼ 10 μM for both amino acids) was competitively inhibited by T3 with a Ki of 2–3 μM (close to the T3 transport Km). Several T3 analogues inhibited system L1 and the T3 transport system similarly. Glutamine uptake and α‐(methylamino)isobutyric acid uptake were, respectively, two and 200 times lower than tryptophan and leucine uptakes. T3 had little effect on the uptakes of glutamine and α‐(methylamino)isobutyric acid. The results indicate that the T3 transport system and system L1 are related.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1993.tb03302.x</identifier><identifier>PMID: 8455031</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Amino Acids - metabolism ; Aminoisobutyric Acids - metabolism ; Animals ; Astrocytes ; Astrocytes - drug effects ; Astrocytes - metabolism ; Biological and medical sciences ; Biological Transport - drug effects ; Cells, Cultured ; Fundamental and applied biological sciences. Psychology ; Isolated neuron and nerve. Neuroglia ; Kinetics ; Leucine - metabolism ; Rats ; Rats, Sprague-Dawley ; System A ; System L ; Thyroid hormones ; Triiodothyronine - pharmacology ; Tryptophan - metabolism ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neurochemistry, 1993-04, Vol.60 (4), p.1407-1413</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1993.tb03302.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1993.tb03302.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4669838$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8455031$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Blondeau, Jean‐Paul</creatorcontrib><creatorcontrib>Beslin, Aline</creatorcontrib><creatorcontrib>Chantoux, Françoise</creatorcontrib><creatorcontrib>Francon, Jacques</creatorcontrib><title>Triiodothyronine Is a High‐Affinity Inhibitor of Amino Acid Transport System L1 in Cultured Astrocytes</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: The relationship between the transport of thyroid hormones and that of amino acids was examined by measuring the uptake of amino acids that are characteristic substrates of systems L, A, and N, and the effect of 3,3′,5‐triiodo‐L‐thyronine (T3) on this uptake, in cultured astrocytes. Tryptophan and leucine uptakes were rapid, Na+‐independent, and efficiently inhibited by T3 (half‐inhibition at ∼ 2 μM). Two Na+‐independent L‐like systems (L1 and L2), common to leucine and aromatic amino acids, were characterized kinetically. System L2 had a low affinity for leucine and tryptophan (Km= 0.3–0.9 mM). The high‐affinity system L1 (Km∼ 10 μM for both amino acids) was competitively inhibited by T3 with a Ki of 2–3 μM (close to the T3 transport Km). Several T3 analogues inhibited system L1 and the T3 transport system similarly. Glutamine uptake and α‐(methylamino)isobutyric acid uptake were, respectively, two and 200 times lower than tryptophan and leucine uptakes. T3 had little effect on the uptakes of glutamine and α‐(methylamino)isobutyric acid. The results indicate that the T3 transport system and system L1 are related.</description><subject>Amino Acids - metabolism</subject><subject>Aminoisobutyric Acids - metabolism</subject><subject>Animals</subject><subject>Astrocytes</subject><subject>Astrocytes - drug effects</subject><subject>Astrocytes - metabolism</subject><subject>Biological and medical sciences</subject><subject>Biological Transport - drug effects</subject><subject>Cells, Cultured</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isolated neuron and nerve. Neuroglia</subject><subject>Kinetics</subject><subject>Leucine - metabolism</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>System A</subject><subject>System L</subject><subject>Thyroid hormones</subject><subject>Triiodothyronine - pharmacology</subject><subject>Tryptophan - metabolism</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kcGO0zAQhi0EWkrhEZAshPaW4ImduD6hqGLZomo5UM6WHTvUVRIX2xGbG4-wz8iTkGqjzmHm8P0aaeZD6AOQHOb6dMqBccgYlCIHIWieNKGUFPnjC7S6opdoRUhRZJSw4jV6E-OJEKhYBTfoZsPKklBYoeMhOOeNT8cp-MENFu8iVvje_Tr--_tUt60bXJrwbjg67ZIP2Le47t3gcd04gw9BDfHsQ8I_pphsj_eA3YC3Y5fGYA2uYwq-mZKNb9GrVnXRvlvmGv28-3LY3mf7719323qfnWjJeVaSVhuhedUYVWjDeVOJCqigghEjiCKCNW3BDdcALROMzrDQar4fQAtm6RrdPu89B_97tDHJ3sXGdp0arB-j5GVFRTm3NXq_BEfdWyPPwfUqTHJ5zcw_LlzFRnXtfGnj4jXGqkps6GaOfX6O_XGdna4YiLyokid58SEvPuRFlVxUyUf57WELjHD6HxEQiKg</recordid><startdate>199304</startdate><enddate>199304</enddate><creator>Blondeau, Jean‐Paul</creator><creator>Beslin, Aline</creator><creator>Chantoux, Françoise</creator><creator>Francon, Jacques</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>199304</creationdate><title>Triiodothyronine Is a High‐Affinity Inhibitor of Amino Acid Transport System L1 in Cultured Astrocytes</title><author>Blondeau, Jean‐Paul ; Beslin, Aline ; Chantoux, Françoise ; Francon, Jacques</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-j3577-50fbd9b76cda2bd77c6961393940d90a094cf27d7b11f49436132bab0311b94e3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acids - metabolism</topic><topic>Aminoisobutyric Acids - metabolism</topic><topic>Animals</topic><topic>Astrocytes</topic><topic>Astrocytes - drug effects</topic><topic>Astrocytes - metabolism</topic><topic>Biological and medical sciences</topic><topic>Biological Transport - drug effects</topic><topic>Cells, Cultured</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isolated neuron and nerve. Neuroglia</topic><topic>Kinetics</topic><topic>Leucine - metabolism</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>System A</topic><topic>System L</topic><topic>Thyroid hormones</topic><topic>Triiodothyronine - pharmacology</topic><topic>Tryptophan - metabolism</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Blondeau, Jean‐Paul</creatorcontrib><creatorcontrib>Beslin, Aline</creatorcontrib><creatorcontrib>Chantoux, Françoise</creatorcontrib><creatorcontrib>Francon, Jacques</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Blondeau, Jean‐Paul</au><au>Beslin, Aline</au><au>Chantoux, Françoise</au><au>Francon, Jacques</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Triiodothyronine Is a High‐Affinity Inhibitor of Amino Acid Transport System L1 in Cultured Astrocytes</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1993-04</date><risdate>1993</risdate><volume>60</volume><issue>4</issue><spage>1407</spage><epage>1413</epage><pages>1407-1413</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: The relationship between the transport of thyroid hormones and that of amino acids was examined by measuring the uptake of amino acids that are characteristic substrates of systems L, A, and N, and the effect of 3,3′,5‐triiodo‐L‐thyronine (T3) on this uptake, in cultured astrocytes. Tryptophan and leucine uptakes were rapid, Na+‐independent, and efficiently inhibited by T3 (half‐inhibition at ∼ 2 μM). Two Na+‐independent L‐like systems (L1 and L2), common to leucine and aromatic amino acids, were characterized kinetically. System L2 had a low affinity for leucine and tryptophan (Km= 0.3–0.9 mM). The high‐affinity system L1 (Km∼ 10 μM for both amino acids) was competitively inhibited by T3 with a Ki of 2–3 μM (close to the T3 transport Km). Several T3 analogues inhibited system L1 and the T3 transport system similarly. Glutamine uptake and α‐(methylamino)isobutyric acid uptake were, respectively, two and 200 times lower than tryptophan and leucine uptakes. T3 had little effect on the uptakes of glutamine and α‐(methylamino)isobutyric acid. The results indicate that the T3 transport system and system L1 are related.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8455031</pmid><doi>10.1111/j.1471-4159.1993.tb03302.x</doi><tpages>7</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Amino Acids - metabolism Aminoisobutyric Acids - metabolism Animals Astrocytes Astrocytes - drug effects Astrocytes - metabolism Biological and medical sciences Biological Transport - drug effects Cells, Cultured Fundamental and applied biological sciences. Psychology Isolated neuron and nerve. Neuroglia Kinetics Leucine - metabolism Rats Rats, Sprague-Dawley System A System L Thyroid hormones Triiodothyronine - pharmacology Tryptophan - metabolism Vertebrates: nervous system and sense organs |
| title | Triiodothyronine Is a High‐Affinity Inhibitor of Amino Acid Transport System L1 in Cultured Astrocytes |
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