Cyclin-dependent regulation of G1 in mammalian fibroblasts
Eukaryotic cells become committed to proliferate during the G1 phase of the cell cycle. In budding yeast, commitment occurs when the catalytic subunit of a protein kinase, encoded by the CDC28 gene (the homolog of the fission yeast cdc2+ gene), binds to a positively acting regulatory subunit, a cycl...
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| Veröffentlicht in: | Science (American Association for the Advancement of Science) 1993-03, Vol.259 (5103), p.1908-1912 |
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| container_end_page | 1912 |
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| container_issue | 5103 |
| container_start_page | 1908 |
| container_title | Science (American Association for the Advancement of Science) |
| container_volume | 259 |
| creator | OHTSUBO, M ROBERTS, J. M |
| description | Eukaryotic cells become committed to proliferate during the G1 phase of the cell cycle. In budding yeast, commitment occurs when the catalytic subunit of a protein kinase, encoded by the CDC28 gene (the homolog of the fission yeast cdc2+ gene), binds to a positively acting regulatory subunit, a cyclin. Related kinases are also required for progression through the G1 phase in higher eukaryotes. The role of cyclins in controlling G1 progression in mammalian cells was tested by construction of fibroblasts that constitutively overexpress human cyclin E. This was found to shorten the duration of G1, decrease cell size, and diminish the serum requirement for the transition from G1 to S phase. These observations show that cyclin levels can be rate-limiting for G1 progression in mammalian cells and suggest that cyclin synthesis may be the target of physiological signals that control cell proliferation. |
| doi_str_mv | 10.1126/science.8384376 |
| format | Article |
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These observations show that cyclin levels can be rate-limiting for G1 progression in mammalian cells and suggest that cyclin synthesis may be the target of physiological signals that control cell proliferation.</description><identifier>ISSN: 0036-8075</identifier><identifier>EISSN: 1095-9203</identifier><identifier>DOI: 10.1126/science.8384376</identifier><identifier>PMID: 8384376</identifier><identifier>CODEN: SCIEAS</identifier><language>eng</language><publisher>Washington, DC: American Association for the Advancement of Science</publisher><subject>Animals ; Biological and medical sciences ; Cell cycle ; Cell cycle, cell proliferation ; Cell Division - physiology ; Cell Line ; Cell physiology ; Cell proliferation ; Cloning, Molecular ; Cyclins - genetics ; Cyclins - physiology ; Cytology ; Fibroblasts - cytology ; Fibroblasts - metabolism ; Flow Cytometry ; Fundamental and applied biological sciences. 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M</creatorcontrib><title>Cyclin-dependent regulation of G1 in mammalian fibroblasts</title><title>Science (American Association for the Advancement of Science)</title><addtitle>Science</addtitle><description>Eukaryotic cells become committed to proliferate during the G1 phase of the cell cycle. In budding yeast, commitment occurs when the catalytic subunit of a protein kinase, encoded by the CDC28 gene (the homolog of the fission yeast cdc2+ gene), binds to a positively acting regulatory subunit, a cyclin. Related kinases are also required for progression through the G1 phase in higher eukaryotes. The role of cyclins in controlling G1 progression in mammalian cells was tested by construction of fibroblasts that constitutively overexpress human cyclin E. This was found to shorten the duration of G1, decrease cell size, and diminish the serum requirement for the transition from G1 to S phase. These observations show that cyclin levels can be rate-limiting for G1 progression in mammalian cells and suggest that cyclin synthesis may be the target of physiological signals that control cell proliferation.</description><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Cell cycle</subject><subject>Cell cycle, cell proliferation</subject><subject>Cell Division - physiology</subject><subject>Cell Line</subject><subject>Cell physiology</subject><subject>Cell proliferation</subject><subject>Cloning, Molecular</subject><subject>Cyclins - genetics</subject><subject>Cyclins - physiology</subject><subject>Cytology</subject><subject>Fibroblasts - cytology</subject><subject>Fibroblasts - metabolism</subject><subject>Flow Cytometry</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>G1 Phase - physiology</subject><subject>Gene Expression</subject><subject>Genetic aspects</subject><subject>Genetic Vectors</subject><subject>Humans</subject><subject>Kanamycin Kinase</subject><subject>Male</subject><subject>Mammals</subject><subject>Molecular and cellular biology</subject><subject>Phosphotransferases - genetics</subject><subject>Rats</subject><subject>Recombinant Fusion Proteins - metabolism</subject><subject>Retroviridae - genetics</subject><subject>S Phase - physiology</subject><subject>Time Factors</subject><subject>Transfection</subject><issn>0036-8075</issn><issn>1095-9203</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqN0kFr2zAUB3BRWros27mngQ-l7FC3kmVJVm9d2NJCaA4du5pn-cmoyHJq2dB--3nUDAI5BB0e6P9DoPceIReM3jCWydtoHAaDNwUvcq7kCVkwqkWqM8pPyYJSLtOCKvGJfI7xhdIp0_ycnM98Qe5W78a7kNa4w1BjGJIem9HD4LqQdDZZs8SFpIW2Be8gJNZVfVd5iEP8Qs4s-Ihf57okz79-_l49pJvt-nF1v0mbXOohlZXN81zVNDNU6UIzXSmrIJeFzZjR3EgJgJihEZxWFWcZCCMECMrQFHxJrj5e3fXd64hxKFsXDXoPAbsxlkpILvn00SW5_oANeCxdsN3Qg2kwYA--C2jddH3PuNCq4P94eoBPp8bWmUP--56fyIBvQwNjjOXj89PRdPvnaPpjfSwt1ps9en2Ims57bLCcprPa7vFvc4vHqsW63PWuhf69nJdkyi_nHKIBb3sIxsX_LJdq6r_mfwFfVcYX</recordid><startdate>19930326</startdate><enddate>19930326</enddate><creator>OHTSUBO, M</creator><creator>ROBERTS, J. 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Psychology</topic><topic>G1 Phase - physiology</topic><topic>Gene Expression</topic><topic>Genetic aspects</topic><topic>Genetic Vectors</topic><topic>Humans</topic><topic>Kanamycin Kinase</topic><topic>Male</topic><topic>Mammals</topic><topic>Molecular and cellular biology</topic><topic>Phosphotransferases - genetics</topic><topic>Rats</topic><topic>Recombinant Fusion Proteins - metabolism</topic><topic>Retroviridae - genetics</topic><topic>S Phase - physiology</topic><topic>Time Factors</topic><topic>Transfection</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>OHTSUBO, M</creatorcontrib><creatorcontrib>ROBERTS, J. 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M</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cyclin-dependent regulation of G1 in mammalian fibroblasts</atitle><jtitle>Science (American Association for the Advancement of Science)</jtitle><addtitle>Science</addtitle><date>1993-03-26</date><risdate>1993</risdate><volume>259</volume><issue>5103</issue><spage>1908</spage><epage>1912</epage><pages>1908-1912</pages><issn>0036-8075</issn><eissn>1095-9203</eissn><coden>SCIEAS</coden><abstract>Eukaryotic cells become committed to proliferate during the G1 phase of the cell cycle. In budding yeast, commitment occurs when the catalytic subunit of a protein kinase, encoded by the CDC28 gene (the homolog of the fission yeast cdc2+ gene), binds to a positively acting regulatory subunit, a cyclin. Related kinases are also required for progression through the G1 phase in higher eukaryotes. The role of cyclins in controlling G1 progression in mammalian cells was tested by construction of fibroblasts that constitutively overexpress human cyclin E. This was found to shorten the duration of G1, decrease cell size, and diminish the serum requirement for the transition from G1 to S phase. These observations show that cyclin levels can be rate-limiting for G1 progression in mammalian cells and suggest that cyclin synthesis may be the target of physiological signals that control cell proliferation.</abstract><cop>Washington, DC</cop><pub>American Association for the Advancement of Science</pub><pmid>8384376</pmid><doi>10.1126/science.8384376</doi><tpages>5</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0036-8075 |
| ispartof | Science (American Association for the Advancement of Science), 1993-03, Vol.259 (5103), p.1908-1912 |
| issn | 0036-8075 1095-9203 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75636300 |
| source | MEDLINE; American Association for the Advancement of Science; Jstor Complete Legacy |
| subjects | Animals Biological and medical sciences Cell cycle Cell cycle, cell proliferation Cell Division - physiology Cell Line Cell physiology Cell proliferation Cloning, Molecular Cyclins - genetics Cyclins - physiology Cytology Fibroblasts - cytology Fibroblasts - metabolism Flow Cytometry Fundamental and applied biological sciences. Psychology G1 Phase - physiology Gene Expression Genetic aspects Genetic Vectors Humans Kanamycin Kinase Male Mammals Molecular and cellular biology Phosphotransferases - genetics Rats Recombinant Fusion Proteins - metabolism Retroviridae - genetics S Phase - physiology Time Factors Transfection |
| title | Cyclin-dependent regulation of G1 in mammalian fibroblasts |
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