Monoclonal antibody against CD11b/CD18 inhibits endotoxin-induced uveitis
To examine the serial expression of cell adhesion molecules in C3H/HeN mice with endotoxin-induced uveitis, and to study the effect of treatment with a monoclonal antibody against Mac-1 on the development of endotoxin-induced uveitis. Immunohistochemical staining was used to document the serial expr...
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| Veröffentlicht in: | Investigative ophthalmology & visual science 1993-03, Vol.34 (3), p.673-681 |
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| creator | Whitcup, SM DeBarge, LR Rosen, H Nussenblatt, RB Chan, CC |
| description | To examine the serial expression of cell adhesion molecules in C3H/HeN mice with endotoxin-induced uveitis, and to study the effect of treatment with a monoclonal antibody against Mac-1 on the development of endotoxin-induced uveitis.
Immunohistochemical staining was used to document the serial expression of Mac-1, intercellular adhesion molecule-1 (ICAM-1), and lymphocyte function-associated antigen-1 (LFA-1) in eyes of C3H/HeN mice with endotoxin-induced uveitis. Then, in two separate experiments, endotoxin-induced uveitis was produced in a total of 48 mice by injecting Salmonella typhimurium endotoxin into one hind footpad. At the time of endotoxin injection, 24 mice received an intraperitoneal injection of anti-Mac-1 antibody and 24 control mice received an intraperitoneal injection of rat IgG. Histopathologic sections of eyes taken 24 hours after endotoxin injection were graded by two masked observers on a scale of 0 to 4.
Intercellular adhesion molecule-1 was first expressed on the ciliary body epithelium 6 hr after endotoxin injection and Mac-1 and LFA-1 were expressed on infiltrating inflammatory cells 12 hr after endotoxin injection. Treatment with anti-MAC-1 antibody significantly inhibited the development of ocular inflammation when compared with treatment with control IgG (P < 0.001).
Intercellular adhesion molecule-1 is expressed in the eye before clinical or histologic signs of inflammation. Furthermore, treatment with antibody against Mac-1 significantly inhibits the development of endotoxin-induced uveitis in mice and suggests that anti-Mac-1 antibody may be useful for treating acute ocular inflammation. |
| format | Article |
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Immunohistochemical staining was used to document the serial expression of Mac-1, intercellular adhesion molecule-1 (ICAM-1), and lymphocyte function-associated antigen-1 (LFA-1) in eyes of C3H/HeN mice with endotoxin-induced uveitis. Then, in two separate experiments, endotoxin-induced uveitis was produced in a total of 48 mice by injecting Salmonella typhimurium endotoxin into one hind footpad. At the time of endotoxin injection, 24 mice received an intraperitoneal injection of anti-Mac-1 antibody and 24 control mice received an intraperitoneal injection of rat IgG. Histopathologic sections of eyes taken 24 hours after endotoxin injection were graded by two masked observers on a scale of 0 to 4.
Intercellular adhesion molecule-1 was first expressed on the ciliary body epithelium 6 hr after endotoxin injection and Mac-1 and LFA-1 were expressed on infiltrating inflammatory cells 12 hr after endotoxin injection. Treatment with anti-MAC-1 antibody significantly inhibited the development of ocular inflammation when compared with treatment with control IgG (P < 0.001).
Intercellular adhesion molecule-1 is expressed in the eye before clinical or histologic signs of inflammation. Furthermore, treatment with antibody against Mac-1 significantly inhibits the development of endotoxin-induced uveitis in mice and suggests that anti-Mac-1 antibody may be useful for treating acute ocular inflammation.</description><identifier>ISSN: 0146-0404</identifier><identifier>EISSN: 1552-5783</identifier><identifier>PMID: 8095493</identifier><identifier>CODEN: IOVSDA</identifier><language>eng</language><publisher>Rockville, MD: ARVO</publisher><subject>Animals ; Antibodies, Monoclonal - therapeutic use ; Antigens, CD - immunology ; Bacterial Toxins ; Biological and medical sciences ; CD18 Antigens ; Cell Adhesion Molecules - immunology ; Ciliary Body - immunology ; Endotoxins ; Female ; Immunoenzyme Techniques ; Immunotherapy ; Macrophage-1 Antigen - immunology ; Medical sciences ; Mice ; Mice, Inbred C3H ; Ophthalmology ; Pigment Epithelium of Eye - immunology ; Uvea diseases ; Uveitis, Anterior - pathology ; Uveitis, Anterior - prevention & control</subject><ispartof>Investigative ophthalmology & visual science, 1993-03, Vol.34 (3), p.673-681</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4670369$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8095493$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Whitcup, SM</creatorcontrib><creatorcontrib>DeBarge, LR</creatorcontrib><creatorcontrib>Rosen, H</creatorcontrib><creatorcontrib>Nussenblatt, RB</creatorcontrib><creatorcontrib>Chan, CC</creatorcontrib><title>Monoclonal antibody against CD11b/CD18 inhibits endotoxin-induced uveitis</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>To examine the serial expression of cell adhesion molecules in C3H/HeN mice with endotoxin-induced uveitis, and to study the effect of treatment with a monoclonal antibody against Mac-1 on the development of endotoxin-induced uveitis.
Immunohistochemical staining was used to document the serial expression of Mac-1, intercellular adhesion molecule-1 (ICAM-1), and lymphocyte function-associated antigen-1 (LFA-1) in eyes of C3H/HeN mice with endotoxin-induced uveitis. Then, in two separate experiments, endotoxin-induced uveitis was produced in a total of 48 mice by injecting Salmonella typhimurium endotoxin into one hind footpad. At the time of endotoxin injection, 24 mice received an intraperitoneal injection of anti-Mac-1 antibody and 24 control mice received an intraperitoneal injection of rat IgG. Histopathologic sections of eyes taken 24 hours after endotoxin injection were graded by two masked observers on a scale of 0 to 4.
Intercellular adhesion molecule-1 was first expressed on the ciliary body epithelium 6 hr after endotoxin injection and Mac-1 and LFA-1 were expressed on infiltrating inflammatory cells 12 hr after endotoxin injection. Treatment with anti-MAC-1 antibody significantly inhibited the development of ocular inflammation when compared with treatment with control IgG (P < 0.001).
Intercellular adhesion molecule-1 is expressed in the eye before clinical or histologic signs of inflammation. Furthermore, treatment with antibody against Mac-1 significantly inhibits the development of endotoxin-induced uveitis in mice and suggests that anti-Mac-1 antibody may be useful for treating acute ocular inflammation.</description><subject>Animals</subject><subject>Antibodies, Monoclonal - therapeutic use</subject><subject>Antigens, CD - immunology</subject><subject>Bacterial Toxins</subject><subject>Biological and medical sciences</subject><subject>CD18 Antigens</subject><subject>Cell Adhesion Molecules - immunology</subject><subject>Ciliary Body - immunology</subject><subject>Endotoxins</subject><subject>Female</subject><subject>Immunoenzyme Techniques</subject><subject>Immunotherapy</subject><subject>Macrophage-1 Antigen - immunology</subject><subject>Medical sciences</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Ophthalmology</subject><subject>Pigment Epithelium of Eye - immunology</subject><subject>Uvea diseases</subject><subject>Uveitis, Anterior - pathology</subject><subject>Uveitis, Anterior - prevention & control</subject><issn>0146-0404</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9z8tOwzAQBVALgUopfAJSFsAuwm8nS1RelYrYwNqaJE5j5NglTgj9eyI1YjOzuEdXMydoSYSgqVAZO0VLTLhMMcf8HF3E-IUxJYTiBVpkOBc8Z0u0eQs-lC54cAn43hahOiSwA-tjn6wfCSnup5kl1je2sH1MjK9CH36tT62vhtJUyfBjbG_jJTqrwUVzNe8V-nx--li_ptv3l836YZs2VIo-lTlITniFK0Ih40xSgRUmWNRKEMahkKCUMTngXNVAaD2dgAtOyqKmMgPCVuju2LvvwvdgYq9bG0vjHHgThqiVkEwSIiZ4PcOhaE2l951toTvo-fcpv5lziCW4ugNf2vjPuFSYyXxit0fW2F0z2s7o2IJzUynR4zgyrpmWirE_oKJuSg</recordid><startdate>19930301</startdate><enddate>19930301</enddate><creator>Whitcup, SM</creator><creator>DeBarge, LR</creator><creator>Rosen, H</creator><creator>Nussenblatt, RB</creator><creator>Chan, CC</creator><general>ARVO</general><general>Association for Research in Vision and Ophtalmology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>7X8</scope></search><sort><creationdate>19930301</creationdate><title>Monoclonal antibody against CD11b/CD18 inhibits endotoxin-induced uveitis</title><author>Whitcup, SM ; DeBarge, LR ; Rosen, H ; Nussenblatt, RB ; Chan, CC</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-h265t-69a6414d0d12a843625070105f75134ab6a77ee9a097fa12f11b0b41cbf268a13</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Antibodies, Monoclonal - therapeutic use</topic><topic>Antigens, CD - immunology</topic><topic>Bacterial Toxins</topic><topic>Biological and medical sciences</topic><topic>CD18 Antigens</topic><topic>Cell Adhesion Molecules - immunology</topic><topic>Ciliary Body - immunology</topic><topic>Endotoxins</topic><topic>Female</topic><topic>Immunoenzyme Techniques</topic><topic>Immunotherapy</topic><topic>Macrophage-1 Antigen - immunology</topic><topic>Medical sciences</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Ophthalmology</topic><topic>Pigment Epithelium of Eye - immunology</topic><topic>Uvea diseases</topic><topic>Uveitis, Anterior - pathology</topic><topic>Uveitis, Anterior - prevention & control</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Whitcup, SM</creatorcontrib><creatorcontrib>DeBarge, LR</creatorcontrib><creatorcontrib>Rosen, H</creatorcontrib><creatorcontrib>Nussenblatt, RB</creatorcontrib><creatorcontrib>Chan, CC</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Whitcup, SM</au><au>DeBarge, LR</au><au>Rosen, H</au><au>Nussenblatt, RB</au><au>Chan, CC</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Monoclonal antibody against CD11b/CD18 inhibits endotoxin-induced uveitis</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>1993-03-01</date><risdate>1993</risdate><volume>34</volume><issue>3</issue><spage>673</spage><epage>681</epage><pages>673-681</pages><issn>0146-0404</issn><eissn>1552-5783</eissn><coden>IOVSDA</coden><abstract>To examine the serial expression of cell adhesion molecules in C3H/HeN mice with endotoxin-induced uveitis, and to study the effect of treatment with a monoclonal antibody against Mac-1 on the development of endotoxin-induced uveitis.
Immunohistochemical staining was used to document the serial expression of Mac-1, intercellular adhesion molecule-1 (ICAM-1), and lymphocyte function-associated antigen-1 (LFA-1) in eyes of C3H/HeN mice with endotoxin-induced uveitis. Then, in two separate experiments, endotoxin-induced uveitis was produced in a total of 48 mice by injecting Salmonella typhimurium endotoxin into one hind footpad. At the time of endotoxin injection, 24 mice received an intraperitoneal injection of anti-Mac-1 antibody and 24 control mice received an intraperitoneal injection of rat IgG. Histopathologic sections of eyes taken 24 hours after endotoxin injection were graded by two masked observers on a scale of 0 to 4.
Intercellular adhesion molecule-1 was first expressed on the ciliary body epithelium 6 hr after endotoxin injection and Mac-1 and LFA-1 were expressed on infiltrating inflammatory cells 12 hr after endotoxin injection. Treatment with anti-MAC-1 antibody significantly inhibited the development of ocular inflammation when compared with treatment with control IgG (P < 0.001).
Intercellular adhesion molecule-1 is expressed in the eye before clinical or histologic signs of inflammation. Furthermore, treatment with antibody against Mac-1 significantly inhibits the development of endotoxin-induced uveitis in mice and suggests that anti-Mac-1 antibody may be useful for treating acute ocular inflammation.</abstract><cop>Rockville, MD</cop><pub>ARVO</pub><pmid>8095493</pmid><tpages>9</tpages></addata></record> |
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| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals |
| subjects | Animals Antibodies, Monoclonal - therapeutic use Antigens, CD - immunology Bacterial Toxins Biological and medical sciences CD18 Antigens Cell Adhesion Molecules - immunology Ciliary Body - immunology Endotoxins Female Immunoenzyme Techniques Immunotherapy Macrophage-1 Antigen - immunology Medical sciences Mice Mice, Inbred C3H Ophthalmology Pigment Epithelium of Eye - immunology Uvea diseases Uveitis, Anterior - pathology Uveitis, Anterior - prevention & control |
| title | Monoclonal antibody against CD11b/CD18 inhibits endotoxin-induced uveitis |
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