Expression of β‐galactosidase from a hybrid promoter: operator element in Escherichia coli

A hybrid trpPO:lacO regulatory sequence was cloned upstream of a promoterless lacZ gene and recombined onto a Λ bacteriophage. Escherichia coli lysogens representing the four possible phenotypes for lacI and trpR were constructed and the synthesis of β‐galactosidase was assayed under various growth...

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Veröffentlicht in:	FEMS microbiology letters 1993-01, Vol.106 (2), p.135-138
Hauptverfasser:	Dixon, Kevin E., Fix, Douglas F.
Format:	Artikel
Sprache:	eng
Schlagworte:	3‐β‐Indoleacrylic acid Bacteriology beta-Galactosidase - biosynthesis Biological and medical sciences Cloning, Molecular Escherichia coli Escherichia coli - enzymology Escherichia coli - genetics Fundamental and applied biological sciences. Psychology Gene expression Genes, Bacterial Genetics Indoles - pharmacology Isoprophyl‐β‐d‐thiogalactoside Lac Operon Lactose operator Microbiology Operator Regions, Genetic Promoter Regions, Genetic Tryptophan - pharmacology Tryptophan promotor / operator
Online-Zugang:	Volltext
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