Procedure for purification of Escherichia coli ribonucleic acid synthesis termination protein .rho

An improved purification procedure is described for the rho transcription termination factor of Escherichia coli. The method involves lysozyme--sodium deoxycholate lysis, Polymin P fractionation, and chromatography on phosphocellulose, poly(uridylic acid)--Sepharose, and AMP--agarose. The method yie...

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Veröffentlicht in:	Biochemistry (Easton) 1981-03, Vol.20 (6), p.1640-1645
Hauptverfasser:	Finger, Lawrence R, Richardson, John P
Format:	Artikel
Sprache:	eng
Schlagworte:	Adenosine Triphosphatases - metabolism Amino Acids - analysis Chromatography Circular Dichroism Escherichia coli - analysis Molecular Weight Poly U Rho Factor - isolation & purification Rho Factor - metabolism Sepharose Transcription Factors - isolation & purification
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