Characterization of the channel formed by the mycobacterial porin in lipid bilayer membranes. Demonstration of voltage gating and of negative point charges at the channel mouth

We studied the channel formed by the mycobacterial porin from the cell wall of Mycobacterium chelonae (Trias, J., Jarlier, V., and Benz, R. (1992) Science 258, 1479-1481) by reconstituting the mycobacterial porin and cell wall extracts in lipid bilayer membranes. The channel exhibited two different...

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Veröffentlicht in:	The Journal of biological chemistry 1993-03, Vol.268 (9), p.6234-6240
Hauptverfasser:	TRIAS, J, BENZ, R
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Outer Membrane Proteins - metabolism Biological and medical sciences Cell physiology characterization Electrophoresis, Polyacrylamide Gel Fundamental and applied biological sciences. Psychology gating Ion Channel Gating Ion Channels - metabolism Lipid Bilayers - metabolism lipid membranes Membrane and intracellular transports Membrane Potentials Molecular and cellular biology Mycobacterium chelonae Mycobacterium chelonae - metabolism Porins voltage
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