Abnormalities of DNA in human osteoarthritic articular cartilage
The normal amount of DNA in human diploid nuclei was determined by the use of the Feulgen reaction measured by microdensitometry. The DNA‐content of nuclei in normal human articular cartilage was determined in nuclei of zones 3 and 4 of cartilage of the femoral head removed from osteoporotic fractur...
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| Veröffentlicht in: | Cell biochemistry and function 1993-03, Vol.11 (1), p.63-69 |
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| container_title | Cell biochemistry and function |
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| creator | Macha, N. Older, J. Bitensky, Lucille Chayen, J. |
| description | The normal amount of DNA in human diploid nuclei was determined by the use of the Feulgen reaction measured by microdensitometry. The DNA‐content of nuclei in normal human articular cartilage was determined in nuclei of zones 3 and 4 of cartilage of the femoral head removed from osteoporotic fractured necks of femur. Analysis of the results indicated that a degree of synthesis of DNA occurred even in these zones of very elderly persons. Results on these zones in the articular cartilage of osteoarthritic joints indicated that different populations occurred. In some there was DNA‐synthesis related to tetraploidy; in others, the DNA was very stable to acid hydrolysis with no sign of biosynthetic activity; in the last group, which contained erosions of the superficial zones, the DNA was unstable to hydrolysis. |
| doi_str_mv | 10.1002/cbf.290110108 |
| format | Article |
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The DNA‐content of nuclei in normal human articular cartilage was determined in nuclei of zones 3 and 4 of cartilage of the femoral head removed from osteoporotic fractured necks of femur. Analysis of the results indicated that a degree of synthesis of DNA occurred even in these zones of very elderly persons. Results on these zones in the articular cartilage of osteoarthritic joints indicated that different populations occurred. In some there was DNA‐synthesis related to tetraploidy; in others, the DNA was very stable to acid hydrolysis with no sign of biosynthetic activity; in the last group, which contained erosions of the superficial zones, the DNA was unstable to hydrolysis.</description><identifier>ISSN: 0263-6484</identifier><identifier>EISSN: 1099-0844</identifier><identifier>DOI: 10.1002/cbf.290110108</identifier><identifier>PMID: 8453738</identifier><identifier>CODEN: CBFUDH</identifier><language>eng</language><publisher>Chichester, UK: John Wiley & Sons, Ltd</publisher><subject>Aged ; Aged, 80 and over ; Biological and medical sciences ; Cartilage, Articular - chemistry ; Cartilage, Articular - pathology ; Cell Nucleus - chemistry ; Diseases of the osteoarticular system ; DNA ; DNA - analysis ; Female ; Femoral Neck Fractures - etiology ; Feulgen ; Fractures, Spontaneous - etiology ; Humans ; Male ; Medical sciences ; microdensitometry ; osteoarthritis ; Osteoarthritis - metabolism ; Osteoarthritis - pathology ; osteoporosis ; Osteoporosis - complications ; Osteoporosis - metabolism ; Osteoporosis - pathology ; Osteoporosis. Osteomalacia. Paget disease</subject><ispartof>Cell biochemistry and function, 1993-03, Vol.11 (1), p.63-69</ispartof><rights>Copyright © 1993 John Wiley & Sons Ltd.</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4038-44a274abfd8e646735c0764ab236fb6f0f48bd356d2d0c75dc7846b549547b053</citedby><cites>FETCH-LOGICAL-c4038-44a274abfd8e646735c0764ab236fb6f0f48bd356d2d0c75dc7846b549547b053</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fcbf.290110108$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fcbf.290110108$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,776,780,1411,27901,27902,45550,45551</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4631280$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8453738$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Macha, N.</creatorcontrib><creatorcontrib>Older, J.</creatorcontrib><creatorcontrib>Bitensky, Lucille</creatorcontrib><creatorcontrib>Chayen, J.</creatorcontrib><title>Abnormalities of DNA in human osteoarthritic articular cartilage</title><title>Cell biochemistry and function</title><addtitle>Cell Biochem. Funct</addtitle><description>The normal amount of DNA in human diploid nuclei was determined by the use of the Feulgen reaction measured by microdensitometry. The DNA‐content of nuclei in normal human articular cartilage was determined in nuclei of zones 3 and 4 of cartilage of the femoral head removed from osteoporotic fractured necks of femur. Analysis of the results indicated that a degree of synthesis of DNA occurred even in these zones of very elderly persons. Results on these zones in the articular cartilage of osteoarthritic joints indicated that different populations occurred. In some there was DNA‐synthesis related to tetraploidy; in others, the DNA was very stable to acid hydrolysis with no sign of biosynthetic activity; in the last group, which contained erosions of the superficial zones, the DNA was unstable to hydrolysis.</description><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Biological and medical sciences</subject><subject>Cartilage, Articular - chemistry</subject><subject>Cartilage, Articular - pathology</subject><subject>Cell Nucleus - chemistry</subject><subject>Diseases of the osteoarticular system</subject><subject>DNA</subject><subject>DNA - analysis</subject><subject>Female</subject><subject>Femoral Neck Fractures - etiology</subject><subject>Feulgen</subject><subject>Fractures, Spontaneous - etiology</subject><subject>Humans</subject><subject>Male</subject><subject>Medical sciences</subject><subject>microdensitometry</subject><subject>osteoarthritis</subject><subject>Osteoarthritis - metabolism</subject><subject>Osteoarthritis - pathology</subject><subject>osteoporosis</subject><subject>Osteoporosis - complications</subject><subject>Osteoporosis - metabolism</subject><subject>Osteoporosis - pathology</subject><subject>Osteoporosis. Osteomalacia. Paget disease</subject><issn>0263-6484</issn><issn>1099-0844</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kEtP3DAURq2KCgbaZZdIWaDuAjfx9SM7hqEMSGhQpb52luPYYMiD2hOV-fcYTTTqipWv_J370CHkSwGnBUB5Zmp3WlZQFFCA_EBmBVRVDhJxj8yg5DTnKPGAHMb4CAAVp7BP9iUyKqickfN53Q-h061fexuzwWWXq3nm--xh7HSfDXFtBx3WDyHlJkuVN2OrQ2beylbf20_ko9NttJ-n94j8vPr2Y3Gd394tbxbz29wgUJkj6lKgrl0jLUcuKDMgePooKXc1d-BQ1g1lvCkbMII1RkjkNcOKoaiB0SPydTv3OQx_RxvXqvPR2LbVvR3GqATjlHKKCcy3oAlDjME69Rx8p8NGFaDejKlkTO2MJf54GjzWnW129KQo5SdTrqPRrQu6Nz7uMOS0KCUkTGyxf761m_d3qsXF1f8HTAf7JPtl16nDk0qeBFO_V0uFv6rVn8vvQjH6ClzLkTw</recordid><startdate>199303</startdate><enddate>199303</enddate><creator>Macha, N.</creator><creator>Older, J.</creator><creator>Bitensky, Lucille</creator><creator>Chayen, J.</creator><general>John Wiley & Sons, Ltd</general><general>Wiley</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199303</creationdate><title>Abnormalities of DNA in human osteoarthritic articular cartilage</title><author>Macha, N. ; Older, J. ; Bitensky, Lucille ; Chayen, J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4038-44a274abfd8e646735c0764ab236fb6f0f48bd356d2d0c75dc7846b549547b053</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Biological and medical sciences</topic><topic>Cartilage, Articular - chemistry</topic><topic>Cartilage, Articular - pathology</topic><topic>Cell Nucleus - chemistry</topic><topic>Diseases of the osteoarticular system</topic><topic>DNA</topic><topic>DNA - analysis</topic><topic>Female</topic><topic>Femoral Neck Fractures - etiology</topic><topic>Feulgen</topic><topic>Fractures, Spontaneous - etiology</topic><topic>Humans</topic><topic>Male</topic><topic>Medical sciences</topic><topic>microdensitometry</topic><topic>osteoarthritis</topic><topic>Osteoarthritis - metabolism</topic><topic>Osteoarthritis - pathology</topic><topic>osteoporosis</topic><topic>Osteoporosis - complications</topic><topic>Osteoporosis - metabolism</topic><topic>Osteoporosis - pathology</topic><topic>Osteoporosis. Osteomalacia. Paget disease</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Macha, N.</creatorcontrib><creatorcontrib>Older, J.</creatorcontrib><creatorcontrib>Bitensky, Lucille</creatorcontrib><creatorcontrib>Chayen, J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Cell biochemistry and function</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Macha, N.</au><au>Older, J.</au><au>Bitensky, Lucille</au><au>Chayen, J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Abnormalities of DNA in human osteoarthritic articular cartilage</atitle><jtitle>Cell biochemistry and function</jtitle><addtitle>Cell Biochem. Funct</addtitle><date>1993-03</date><risdate>1993</risdate><volume>11</volume><issue>1</issue><spage>63</spage><epage>69</epage><pages>63-69</pages><issn>0263-6484</issn><eissn>1099-0844</eissn><coden>CBFUDH</coden><abstract>The normal amount of DNA in human diploid nuclei was determined by the use of the Feulgen reaction measured by microdensitometry. The DNA‐content of nuclei in normal human articular cartilage was determined in nuclei of zones 3 and 4 of cartilage of the femoral head removed from osteoporotic fractured necks of femur. Analysis of the results indicated that a degree of synthesis of DNA occurred even in these zones of very elderly persons. Results on these zones in the articular cartilage of osteoarthritic joints indicated that different populations occurred. In some there was DNA‐synthesis related to tetraploidy; in others, the DNA was very stable to acid hydrolysis with no sign of biosynthetic activity; in the last group, which contained erosions of the superficial zones, the DNA was unstable to hydrolysis.</abstract><cop>Chichester, UK</cop><pub>John Wiley & Sons, Ltd</pub><pmid>8453738</pmid><doi>10.1002/cbf.290110108</doi><tpages>7</tpages></addata></record> |
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| ispartof | Cell biochemistry and function, 1993-03, Vol.11 (1), p.63-69 |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aged Aged, 80 and over Biological and medical sciences Cartilage, Articular - chemistry Cartilage, Articular - pathology Cell Nucleus - chemistry Diseases of the osteoarticular system DNA DNA - analysis Female Femoral Neck Fractures - etiology Feulgen Fractures, Spontaneous - etiology Humans Male Medical sciences microdensitometry osteoarthritis Osteoarthritis - metabolism Osteoarthritis - pathology osteoporosis Osteoporosis - complications Osteoporosis - metabolism Osteoporosis - pathology Osteoporosis. Osteomalacia. Paget disease |
| title | Abnormalities of DNA in human osteoarthritic articular cartilage |
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