Fine-needle aspiration biopsies of metastatic neuroendocrine tumors in liver: Use of transmission electron microscopy and protein A gold technique in diagnosis
Five fine‐needle aspiration biopsies of liver were studied to evaluate the use of electron microscopy and protein A gold technique in the diagnosis of metastatic neuroendocrine tumors. All five cases had cytologic features suggestive of neuroendocrine tumors and neurosecretory granules on ultrastruc...
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| Veröffentlicht in: | Diagnostic cytopathology 1993-01, Vol.9 (1), p.13-19 |
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| creator | Ravinsky, Esther Quinonez, Guillermo E. Diocee, Malkit S. |
| description | Five fine‐needle aspiration biopsies of liver were studied to evaluate the use of electron microscopy and protein A gold technique in the diagnosis of metastatic neuroendocrine tumors. All five cases had cytologic features suggestive of neuroendocrine tumors and neurosecretory granules on ultrastructural examination.
The aspirates were stained by the immunoperoxidase technique and the postembedding protein A gold technique using the antibody to chromogranin A. Three cases were positive for chromogranin A by both immunoperoxidase and protein A gold techniques. Two cases were positive by protein A gold technique and negative by immunoperoxidase technique.
Confirmation of neurosecretory granules by immunochemistry is ideal, since ultrastructurally they can be mimicked by other electron‐dense inclusions. Advantage of the protein A gold technique is the use of the same material for both ultrastructural evaluation and the protein A gold technique. |
| doi_str_mv | 10.1002/dc.2840090104 |
| format | Article |
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The aspirates were stained by the immunoperoxidase technique and the postembedding protein A gold technique using the antibody to chromogranin A. Three cases were positive for chromogranin A by both immunoperoxidase and protein A gold techniques. Two cases were positive by protein A gold technique and negative by immunoperoxidase technique.
Confirmation of neurosecretory granules by immunochemistry is ideal, since ultrastructurally they can be mimicked by other electron‐dense inclusions. Advantage of the protein A gold technique is the use of the same material for both ultrastructural evaluation and the protein A gold technique.</description><identifier>ISSN: 8755-1039</identifier><identifier>EISSN: 1097-0339</identifier><identifier>DOI: 10.1002/dc.2840090104</identifier><identifier>PMID: 8458276</identifier><identifier>CODEN: DICYE7</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Adult ; Aged ; Biological and medical sciences ; Biopsy, Needle ; Chromogranin A ; Diagnostic electron microscopy ; Female ; Gastroenterology. Liver. Pancreas. Abdomen ; Hepatic tumors ; Humans ; Immunochemistry ; Immunohistochemistry ; Liver Neoplasms - pathology ; Liver Neoplasms - secondary ; Liver Neoplasms - ultrastructure ; Liver. Biliary tract. Portal circulation. Exocrine pancreas ; Male ; Medical sciences ; Microscopy, Electron ; Middle Aged ; Neoplasms - pathology ; Neurosecretory granules ; Neurosecretory Systems - pathology ; Postembedding immunogold technique ; Tumors</subject><ispartof>Diagnostic cytopathology, 1993-01, Vol.9 (1), p.13-19</ispartof><rights>Copyright © 1993 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4034-9dc2f28951b9ce9f78282583740713517c4228f26d4db4b065be4f3ea3bb1a503</citedby><cites>FETCH-LOGICAL-c4034-9dc2f28951b9ce9f78282583740713517c4228f26d4db4b065be4f3ea3bb1a503</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fdc.2840090104$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fdc.2840090104$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4662945$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8458276$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ravinsky, Esther</creatorcontrib><creatorcontrib>Quinonez, Guillermo E.</creatorcontrib><creatorcontrib>Diocee, Malkit S.</creatorcontrib><title>Fine-needle aspiration biopsies of metastatic neuroendocrine tumors in liver: Use of transmission electron microscopy and protein A gold technique in diagnosis</title><title>Diagnostic cytopathology</title><addtitle>Diagn. Cytopathol</addtitle><description>Five fine‐needle aspiration biopsies of liver were studied to evaluate the use of electron microscopy and protein A gold technique in the diagnosis of metastatic neuroendocrine tumors. All five cases had cytologic features suggestive of neuroendocrine tumors and neurosecretory granules on ultrastructural examination.
The aspirates were stained by the immunoperoxidase technique and the postembedding protein A gold technique using the antibody to chromogranin A. Three cases were positive for chromogranin A by both immunoperoxidase and protein A gold techniques. Two cases were positive by protein A gold technique and negative by immunoperoxidase technique.
Confirmation of neurosecretory granules by immunochemistry is ideal, since ultrastructurally they can be mimicked by other electron‐dense inclusions. Advantage of the protein A gold technique is the use of the same material for both ultrastructural evaluation and the protein A gold technique.</description><subject>Adult</subject><subject>Aged</subject><subject>Biological and medical sciences</subject><subject>Biopsy, Needle</subject><subject>Chromogranin A</subject><subject>Diagnostic electron microscopy</subject><subject>Female</subject><subject>Gastroenterology. Liver. Pancreas. Abdomen</subject><subject>Hepatic tumors</subject><subject>Humans</subject><subject>Immunochemistry</subject><subject>Immunohistochemistry</subject><subject>Liver Neoplasms - pathology</subject><subject>Liver Neoplasms - secondary</subject><subject>Liver Neoplasms - ultrastructure</subject><subject>Liver. Biliary tract. Portal circulation. Exocrine pancreas</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Microscopy, Electron</subject><subject>Middle Aged</subject><subject>Neoplasms - pathology</subject><subject>Neurosecretory granules</subject><subject>Neurosecretory Systems - pathology</subject><subject>Postembedding immunogold technique</subject><subject>Tumors</subject><issn>8755-1039</issn><issn>1097-0339</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kc1u1DAUhSMEKkNhyRLJC8Quxb9xwq6a0oKoipCmgp3l2DfFkNhT3wwwT8Or4tGMBrFhZcv3O8fHPlX1nNEzRil_7d0ZbyWlHWVUPqgWjHa6pkJ0D6tFq5WqGRXd4-oJ4jdaKM6ak-qklarlullUvy9DhDoC-BGIxXXIdg4pkj6kNQZAkgYywWxxLueORNjkBNEnl4uOzJspZSQhkjH8gPyG3CLsFHO2EaeAuLOCEdycy2YKLid0ab0lNnqyzmmGIj0nd2n0ZAb3NYb7DezsfLB3MWHAp9WjwY4Izw7raXV7-Xa1fFdff7x6vzy_rp2kQtadd3zgbadY3znoBt3ylqtWaEk1E4ppJzlvB9546XvZ00b1IAcBVvQ9s4qK0-rV3rekKhlwNiW-g3G0EdIGjVaN4JrrAtZ7cPcWzDCYdQ6TzVvDqNkVYrwzfwsp_IuD8aafwB_pQwNl_vIwt-jsOJSfcwGPmGwa3klVML3HfoYRtv-_01ws_wlwCBxwhl9Hpc3fTaOFVubzzZVZ0Q-ri5vmi_kk_gAWwbUc</recordid><startdate>199301</startdate><enddate>199301</enddate><creator>Ravinsky, Esther</creator><creator>Quinonez, Guillermo E.</creator><creator>Diocee, Malkit S.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199301</creationdate><title>Fine-needle aspiration biopsies of metastatic neuroendocrine tumors in liver: Use of transmission electron microscopy and protein A gold technique in diagnosis</title><author>Ravinsky, Esther ; Quinonez, Guillermo E. ; Diocee, Malkit S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4034-9dc2f28951b9ce9f78282583740713517c4228f26d4db4b065be4f3ea3bb1a503</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Adult</topic><topic>Aged</topic><topic>Biological and medical sciences</topic><topic>Biopsy, Needle</topic><topic>Chromogranin A</topic><topic>Diagnostic electron microscopy</topic><topic>Female</topic><topic>Gastroenterology. Liver. Pancreas. Abdomen</topic><topic>Hepatic tumors</topic><topic>Humans</topic><topic>Immunochemistry</topic><topic>Immunohistochemistry</topic><topic>Liver Neoplasms - pathology</topic><topic>Liver Neoplasms - secondary</topic><topic>Liver Neoplasms - ultrastructure</topic><topic>Liver. Biliary tract. Portal circulation. Exocrine pancreas</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Microscopy, Electron</topic><topic>Middle Aged</topic><topic>Neoplasms - pathology</topic><topic>Neurosecretory granules</topic><topic>Neurosecretory Systems - pathology</topic><topic>Postembedding immunogold technique</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ravinsky, Esther</creatorcontrib><creatorcontrib>Quinonez, Guillermo E.</creatorcontrib><creatorcontrib>Diocee, Malkit S.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Diagnostic cytopathology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ravinsky, Esther</au><au>Quinonez, Guillermo E.</au><au>Diocee, Malkit S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Fine-needle aspiration biopsies of metastatic neuroendocrine tumors in liver: Use of transmission electron microscopy and protein A gold technique in diagnosis</atitle><jtitle>Diagnostic cytopathology</jtitle><addtitle>Diagn. Cytopathol</addtitle><date>1993-01</date><risdate>1993</risdate><volume>9</volume><issue>1</issue><spage>13</spage><epage>19</epage><pages>13-19</pages><issn>8755-1039</issn><eissn>1097-0339</eissn><coden>DICYE7</coden><abstract>Five fine‐needle aspiration biopsies of liver were studied to evaluate the use of electron microscopy and protein A gold technique in the diagnosis of metastatic neuroendocrine tumors. All five cases had cytologic features suggestive of neuroendocrine tumors and neurosecretory granules on ultrastructural examination.
The aspirates were stained by the immunoperoxidase technique and the postembedding protein A gold technique using the antibody to chromogranin A. Three cases were positive for chromogranin A by both immunoperoxidase and protein A gold techniques. Two cases were positive by protein A gold technique and negative by immunoperoxidase technique.
Confirmation of neurosecretory granules by immunochemistry is ideal, since ultrastructurally they can be mimicked by other electron‐dense inclusions. Advantage of the protein A gold technique is the use of the same material for both ultrastructural evaluation and the protein A gold technique.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8458276</pmid><doi>10.1002/dc.2840090104</doi><tpages>7</tpages></addata></record> |
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| subjects | Adult Aged Biological and medical sciences Biopsy, Needle Chromogranin A Diagnostic electron microscopy Female Gastroenterology. Liver. Pancreas. Abdomen Hepatic tumors Humans Immunochemistry Immunohistochemistry Liver Neoplasms - pathology Liver Neoplasms - secondary Liver Neoplasms - ultrastructure Liver. Biliary tract. Portal circulation. Exocrine pancreas Male Medical sciences Microscopy, Electron Middle Aged Neoplasms - pathology Neurosecretory granules Neurosecretory Systems - pathology Postembedding immunogold technique Tumors |
| title | Fine-needle aspiration biopsies of metastatic neuroendocrine tumors in liver: Use of transmission electron microscopy and protein A gold technique in diagnosis |
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