Control of Selectivity via Nanochemistry: Monolithic Capillary Column Containing Hydroxyapatite Nanoparticles for Separation of Proteins and Enrichment of Phosphopeptides

New monolithic capillary columns with embedded commercial hydroxyapatite nanoparticles have been developed and used for protein separation and selective enrichment of phosphopeptides. The rod-shaped hydroxyapatite nanoparticles were incorporated into the poly(2-hydroxyethyl methacrylate-co-ethylene...

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Veröffentlicht in:	Analytical chemistry (Washington) 2010-10, Vol.82 (19), p.8335-8341
Hauptverfasser:	Krenkova, Jana, Lacher, Nathan A, Svec, Frantisek
Format:	Artikel
Sprache:	eng
Schlagworte:	Analytical chemistry Antibodies, Monoclonal - chemistry Antibodies, Monoclonal - isolation & purification Caseins - chemistry Caseins - isolation & purification Chemical compounds Chromatography, High Pressure Liquid - methods Cytochromes c - chemistry Cytochromes c - isolation & purification Durapatite - chemistry Muramidase - chemistry Muramidase - isolation & purification Myoglobin - chemistry Myoglobin - isolation & purification Nanoparticles Nanoparticles - chemistry Nanoparticles - ultrastructure Ovalbumin - chemistry Ovalbumin - isolation & purification Peptides Phosphopeptides - chemistry Phosphopeptides - isolation & purification Polymerization Polymethacrylic Acids - chemistry Protein Binding Proteins Proteins - chemistry Proteins - isolation & purification Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
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