Development of a selective and sensitive polymerase chain reaction assay for the detection of Mycoplasma pirum

A new assay using the polymerase chain reaction to amplify a 173‐nucleotide DNA fragment within the 16S ribosomal RNA gene of Mycoplasma pirum has been developed. The assay selectively amplified DNA from all strains of M. pirum tested with a high level of sensitivity, even in a context of human DNA....

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Veröffentlicht in:	FEMS microbiology letters 1993-02, Vol.106 (3), p.327-333
Hauptverfasser:	Grau, Odile, Kovacic, Rémi, Griffais, Rémy, Montagnier, Luc
Format:	Artikel
Sprache:	eng
Schlagworte:	16S ribosomal DNA AIDS/HIV Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences DNA Probes DNA, Bacterial - genetics Evaluation Studies as Topic Fundamental and applied biological sciences. Psychology HIV Seropositivity - microbiology Humans Microbiology Molecular Sequence Data Mycoplasma - genetics Mycoplasma - isolation & purification Mycoplasma pirum Polymerase chain reaction Polymerase Chain Reaction - methods Polymerase Chain Reaction - statistics & numerical data Sensitivity and Specificity
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