Deletion analysis of the essentiality of penicillin‐binding proteins 1A, 2B and 2X of Streptococcus pneumoniae

An internal fragment from each of the penicillinebinding protein (PBP) 1A, 2B and 2X genes of Streptococcus pneumoniae, which included the region encoding the active‐site serine residue, was replaced by a fragment encoding spectinomycin resistance. The resulting constructs were tested for their abil...

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