Deletion analysis of the essentiality of penicillin‐binding proteins 1A, 2B and 2X of Streptococcus pneumoniae
An internal fragment from each of the penicillinebinding protein (PBP) 1A, 2B and 2X genes of Streptococcus pneumoniae, which included the region encoding the active‐site serine residue, was replaced by a fragment encoding spectinomycin resistance. The resulting constructs were tested for their abil...
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| Veröffentlicht in: | FEMS microbiology letters 1993-01, Vol.106 (2), p.171-175 |
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| container_title | FEMS microbiology letters |
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| creator | Kell, Christopher M. Sharma, Umender K. Dowson, Christopher G. Town, Christine Balganesh, Tanjore S. Spratt, Brian G. |
| description | An internal fragment from each of the penicillinebinding protein (PBP) 1A, 2B and 2X genes of Streptococcus pneumoniae, which included the region encoding the active‐site serine residue, was replaced by a fragment encoding spectinomycin resistance. The resulting constructs were tested for their ability to transform S. pneumoniae strain R6 to spectinomycin resistance. Spectinomycin‐resistant transformants could not be obtained using either the inactivated PBP 2X or 2B genes, suggesting that deletion of either of these genes was a lethal event, but they were readily obtained using the inactivated PBP 1A gene. Analysis using the polymerase chain reaction confirmed that the latter transformants had replaced their chromosomal copy of the PBP 1A gene with the inactivated copy of the gene. Deletion of the PBP 1A gene was therefore tolerated under laboratory conditions and appeared to have little effect on growth or susceptibility to benzylpenicillin. |
| doi_str_mv | 10.1111/j.1574-6968.1993.tb05954.x |
| format | Article |
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The resulting constructs were tested for their ability to transform S. pneumoniae strain R6 to spectinomycin resistance. Spectinomycin‐resistant transformants could not be obtained using either the inactivated PBP 2X or 2B genes, suggesting that deletion of either of these genes was a lethal event, but they were readily obtained using the inactivated PBP 1A gene. Analysis using the polymerase chain reaction confirmed that the latter transformants had replaced their chromosomal copy of the PBP 1A gene with the inactivated copy of the gene. 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Psychology ; Gene Deletion ; Genes, Bacterial ; Genetic deletion ; Genetics ; Hexosyltransferases ; Microbiology ; Muramoylpentapeptide Carboxypeptidase - genetics ; Penicillin-Binding Proteins ; Penicillin‐binding protein ; Peptidyl Transferases ; Plasmids ; Streptococcus pneumoniae ; Streptococcus pneumoniae - genetics</subject><ispartof>FEMS microbiology letters, 1993-01, Vol.106 (2), p.171-175</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3441-4220c0af2b25dd5da730d955c1313a45877d2e0e3ca551cf561c02e904f49bc83</citedby><cites>FETCH-LOGICAL-c3441-4220c0af2b25dd5da730d955c1313a45877d2e0e3ca551cf561c02e904f49bc83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1574-6968.1993.tb05954.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1574-6968.1993.tb05954.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4587310$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8454182$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kell, Christopher M.</creatorcontrib><creatorcontrib>Sharma, Umender K.</creatorcontrib><creatorcontrib>Dowson, Christopher G.</creatorcontrib><creatorcontrib>Town, Christine</creatorcontrib><creatorcontrib>Balganesh, Tanjore S.</creatorcontrib><creatorcontrib>Spratt, Brian G.</creatorcontrib><title>Deletion analysis of the essentiality of penicillin‐binding proteins 1A, 2B and 2X of Streptococcus pneumoniae</title><title>FEMS microbiology letters</title><addtitle>FEMS Microbiol Lett</addtitle><description>An internal fragment from each of the penicillinebinding protein (PBP) 1A, 2B and 2X genes of Streptococcus pneumoniae, which included the region encoding the active‐site serine residue, was replaced by a fragment encoding spectinomycin resistance. The resulting constructs were tested for their ability to transform S. pneumoniae strain R6 to spectinomycin resistance. Spectinomycin‐resistant transformants could not be obtained using either the inactivated PBP 2X or 2B genes, suggesting that deletion of either of these genes was a lethal event, but they were readily obtained using the inactivated PBP 1A gene. Analysis using the polymerase chain reaction confirmed that the latter transformants had replaced their chromosomal copy of the PBP 1A gene with the inactivated copy of the gene. Deletion of the PBP 1A gene was therefore tolerated under laboratory conditions and appeared to have little effect on growth or susceptibility to benzylpenicillin.</description><subject>Bacterial Proteins</subject><subject>Bacteriology</subject><subject>Biological and medical sciences</subject><subject>Carrier Proteins - genetics</subject><subject>Cefotaxime - pharmacology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Deletion</subject><subject>Genes, Bacterial</subject><subject>Genetic deletion</subject><subject>Genetics</subject><subject>Hexosyltransferases</subject><subject>Microbiology</subject><subject>Muramoylpentapeptide Carboxypeptidase - genetics</subject><subject>Penicillin-Binding Proteins</subject><subject>Penicillin‐binding protein</subject><subject>Peptidyl Transferases</subject><subject>Plasmids</subject><subject>Streptococcus pneumoniae</subject><subject>Streptococcus pneumoniae - genetics</subject><issn>0378-1097</issn><issn>1574-6968</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkctu1DAUhq2qqEwLj4BkIdRVE3xNYhaVSqGANIgFIHVnOc5J65HHSWNHdHY8As_Ik5Bootmino2l83_n4vMj9JqSnE7xdpNTWYqsUEWVU6V4nmoilRT54xFaHaRjtCK8rDJKVPkcnca4IYQIRooTdFIJKWjFVqj_AB6S6wI2wfhddBF3LU73gCFGCMkZ79JuzvUQnHXeu_D395_ahcaFO9wPXQIXIqZXF5i9n5o0mN3O-Pc0QJ8621k7RtwHGLddcAZeoGet8RFeLu8Z-nnz8cf152z97dOX66t1ZrkQNBOMEUtMy2omm0Y2puSkUVJayik3QlZl2TAgwK2RktpWFtQSBoqIVqjaVvwMne_7Tis-jBCT3rpowXsToBujLmXBKlWo_4K0kJxLWUzguz1ohy7GAVrdD25rhp2mRM--6I2ej6_n4-vZF734oh-n4lfLlLHeQnMoXYyY9DeLbqI1vh1MsC4esPnDnJIJu9xjv5yH3RMW0Ddf17Sk_B-gcqtc</recordid><startdate>19930115</startdate><enddate>19930115</enddate><creator>Kell, Christopher M.</creator><creator>Sharma, Umender K.</creator><creator>Dowson, Christopher G.</creator><creator>Town, Christine</creator><creator>Balganesh, Tanjore S.</creator><creator>Spratt, Brian G.</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QL</scope><scope>C1K</scope><scope>7X8</scope></search><sort><creationdate>19930115</creationdate><title>Deletion analysis of the essentiality of penicillin‐binding proteins 1A, 2B and 2X of Streptococcus pneumoniae</title><author>Kell, Christopher M. ; Sharma, Umender K. ; Dowson, Christopher G. ; Town, Christine ; Balganesh, Tanjore S. ; Spratt, Brian G.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3441-4220c0af2b25dd5da730d955c1313a45877d2e0e3ca551cf561c02e904f49bc83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Bacterial Proteins</topic><topic>Bacteriology</topic><topic>Biological and medical sciences</topic><topic>Carrier Proteins - genetics</topic><topic>Cefotaxime - pharmacology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Deletion</topic><topic>Genes, Bacterial</topic><topic>Genetic deletion</topic><topic>Genetics</topic><topic>Hexosyltransferases</topic><topic>Microbiology</topic><topic>Muramoylpentapeptide Carboxypeptidase - genetics</topic><topic>Penicillin-Binding Proteins</topic><topic>Penicillin‐binding protein</topic><topic>Peptidyl Transferases</topic><topic>Plasmids</topic><topic>Streptococcus pneumoniae</topic><topic>Streptococcus pneumoniae - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kell, Christopher M.</creatorcontrib><creatorcontrib>Sharma, Umender K.</creatorcontrib><creatorcontrib>Dowson, Christopher G.</creatorcontrib><creatorcontrib>Town, Christine</creatorcontrib><creatorcontrib>Balganesh, Tanjore S.</creatorcontrib><creatorcontrib>Spratt, Brian G.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Environmental Sciences and Pollution Management</collection><collection>MEDLINE - Academic</collection><jtitle>FEMS microbiology letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kell, Christopher M.</au><au>Sharma, Umender K.</au><au>Dowson, Christopher G.</au><au>Town, Christine</au><au>Balganesh, Tanjore S.</au><au>Spratt, Brian G.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Deletion analysis of the essentiality of penicillin‐binding proteins 1A, 2B and 2X of Streptococcus pneumoniae</atitle><jtitle>FEMS microbiology letters</jtitle><addtitle>FEMS Microbiol Lett</addtitle><date>1993-01-15</date><risdate>1993</risdate><volume>106</volume><issue>2</issue><spage>171</spage><epage>175</epage><pages>171-175</pages><issn>0378-1097</issn><eissn>1574-6968</eissn><coden>FMLED7</coden><abstract>An internal fragment from each of the penicillinebinding protein (PBP) 1A, 2B and 2X genes of Streptococcus pneumoniae, which included the region encoding the active‐site serine residue, was replaced by a fragment encoding spectinomycin resistance. The resulting constructs were tested for their ability to transform S. pneumoniae strain R6 to spectinomycin resistance. Spectinomycin‐resistant transformants could not be obtained using either the inactivated PBP 2X or 2B genes, suggesting that deletion of either of these genes was a lethal event, but they were readily obtained using the inactivated PBP 1A gene. Analysis using the polymerase chain reaction confirmed that the latter transformants had replaced their chromosomal copy of the PBP 1A gene with the inactivated copy of the gene. Deletion of the PBP 1A gene was therefore tolerated under laboratory conditions and appeared to have little effect on growth or susceptibility to benzylpenicillin.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>8454182</pmid><doi>10.1111/j.1574-6968.1993.tb05954.x</doi><tpages>5</tpages></addata></record> |
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| identifier | ISSN: 0378-1097 |
| ispartof | FEMS microbiology letters, 1993-01, Vol.106 (2), p.171-175 |
| issn | 0378-1097 1574-6968 |
| language | eng |
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| source | MEDLINE; Oxford University Press Archive; Access via Wiley Online Library; Alma/SFX Local Collection |
| subjects | Bacterial Proteins Bacteriology Biological and medical sciences Carrier Proteins - genetics Cefotaxime - pharmacology Fundamental and applied biological sciences. Psychology Gene Deletion Genes, Bacterial Genetic deletion Genetics Hexosyltransferases Microbiology Muramoylpentapeptide Carboxypeptidase - genetics Penicillin-Binding Proteins Penicillin‐binding protein Peptidyl Transferases Plasmids Streptococcus pneumoniae Streptococcus pneumoniae - genetics |
| title | Deletion analysis of the essentiality of penicillin‐binding proteins 1A, 2B and 2X of Streptococcus pneumoniae |
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