The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast

The Saccharomyces cerevisiae vacuolar protein sorting mutant, vps16, exhibits pleiotropic defects in vacuolar protein targeting and vacuole morphology. To understand the role of the VPS16 gene in vacuolar protein sorting and organelle assembly, a vps16 ts mutant was used to clone the wild-type gene....

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Veröffentlicht in:The Journal of biological chemistry 1993-03, Vol.268 (7), p.4953-4962
Hauptverfasser: Horazdovsky, B.F. (California Institute of Technology, Pasadena, CA), Emr, S.D
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creator Horazdovsky, B.F. (California Institute of Technology, Pasadena, CA)
Emr, S.D
description The Saccharomyces cerevisiae vacuolar protein sorting mutant, vps16, exhibits pleiotropic defects in vacuolar protein targeting and vacuole morphology. To understand the role of the VPS16 gene in vacuolar protein sorting and organelle assembly, a vps16 ts mutant was used to clone the wild-type gene. DNA sequence analysis identified a single open reading frame within a vps16 complementing DNA fragment, capable of encoding a protein of 92,000 Da. Hydrophobicity analysis indicates that the Vps16 protein (Vps16p) is hydrophilic and contains no obvious signal sequence or membrane spanning domains. Gene disruption experiments have shown that VPS16 is not essential. delta vps16 cells exhibit, 1) a severe defect in vacuolar protein sorting; 2) a ts growth defect; 3) a grossly abnormal vacuole morphology, no normal vacuole compartment(s) is present; and 4) a defect in alpha-factor processing. A trpE-vps16 fusion protein has been used to generate polyclonal antiserum. This antiserum detects an unglycosylated protein of 90,000 Da. Subcellular fractionation studies indicate that the vast majority of the VPS16 gene product is associated with a particulate cell fraction. This association is resistant to detergent and salt extractions, but Vps16p can be extracted with 6 M urea and alkali buffer. In addition, overexpression of Vps16p appears to saturate the association sites available in this sedimentable structure. These data indicate that Vps16p may be specifically associated with a large protein complex, or with a limited number of sites on cytoskeletal elements of the cell
doi_str_mv 10.1016/S0021-9258(18)53488-8
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Gene disruption experiments have shown that VPS16 is not essential. delta vps16 cells exhibit, 1) a severe defect in vacuolar protein sorting; 2) a ts growth defect; 3) a grossly abnormal vacuole morphology, no normal vacuole compartment(s) is present; and 4) a defect in alpha-factor processing. A trpE-vps16 fusion protein has been used to generate polyclonal antiserum. This antiserum detects an unglycosylated protein of 90,000 Da. Subcellular fractionation studies indicate that the vast majority of the VPS16 gene product is associated with a particulate cell fraction. This association is resistant to detergent and salt extractions, but Vps16p can be extracted with 6 M urea and alkali buffer. In addition, overexpression of Vps16p appears to saturate the association sites available in this sedimentable structure. 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DNA sequence analysis identified a single open reading frame within a vps16 complementing DNA fragment, capable of encoding a protein of 92,000 Da. Hydrophobicity analysis indicates that the Vps16 protein (Vps16p) is hydrophilic and contains no obvious signal sequence or membrane spanning domains. Gene disruption experiments have shown that VPS16 is not essential. delta vps16 cells exhibit, 1) a severe defect in vacuolar protein sorting; 2) a ts growth defect; 3) a grossly abnormal vacuole morphology, no normal vacuole compartment(s) is present; and 4) a defect in alpha-factor processing. A trpE-vps16 fusion protein has been used to generate polyclonal antiserum. This antiserum detects an unglycosylated protein of 90,000 Da. Subcellular fractionation studies indicate that the vast majority of the VPS16 gene product is associated with a particulate cell fraction. This association is resistant to detergent and salt extractions, but Vps16p can be extracted with 6 M urea and alkali buffer. In addition, overexpression of Vps16p appears to saturate the association sites available in this sedimentable structure. These data indicate that Vps16p may be specifically associated with a large protein complex, or with a limited number of sites on cytoskeletal elements of the cell</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8444873</pmid><doi>10.1016/S0021-9258(18)53488-8</doi><tpages>10</tpages><oa>free_for_read</oa></addata></record>
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subjects Amino Acid Sequence
Base Sequence
Biological and medical sciences
Cloning, Molecular
DNA, Fungal
Fundamental and applied biological sciences. Psychology
Fungal Proteins - genetics
Fungal Proteins - metabolism
GENE
gene products
GENES
Genes, Fungal
Genes. Genome
Membrane Proteins - metabolism
METABOLISME DES PROTEINES
METABOLISMO PROTEICO
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
nucleotide sequence
PEPTIDASAS
PEPTIDASE
PROTEINAS
PROTEINE
purification
SACCHAROMYCES CEREVISIAE
Saccharomyces cerevisiae - genetics
Saccharomyces cerevisiae - metabolism
Saccharomyces cerevisiae Proteins
SECRECION
SECRETION
SECUENCIA NUCLEICA
SEQUENCE NUCLEIQUE
VACUOLA
VACUOLE
Vacuoles - metabolism
Vesicular Transport Proteins
vps16 gene
title The VPS16 gene product associates with a sedimentable protein complex and is essential for vacuolar protein sorting in yeast
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