Cloning and characterization of a novel zinc-finger protein-encoding cDNA from the mouse eye lens
Zinc fingers (Zf) are a common structural motif found in many nucleic acid-binding proteins. In an effort to identify potential transcription factors in the mouse eye lens, we have isolated a Zf-containing clone from a newborn mouse lens cDNA library. The clone, named pMLZ-4, is 4.5 kb in length and...
Gespeichert in:
| Veröffentlicht in: | Gene 1993-02, Vol.124 (2), p.207-214 |
|---|---|
| Hauptverfasser: | , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 214 |
|---|---|
| container_issue | 2 |
| container_start_page | 207 |
| container_title | Gene |
| container_volume | 124 |
| creator | Brady, James P. Piatigorsky, Joram |
| description | Zinc fingers (Zf) are a common structural motif found in many nucleic acid-binding proteins. In an effort to identify potential transcription factors in the mouse eye lens, we have isolated a
Zf-containing clone from a newborn mouse lens cDNA library. The clone, named pMLZ-4, is 4.5 kb in length and contains an open reading frame of 1073 bp. The putative pMLZ-4 protein consists of a short, N-terminal acidic domain followed by twelve tandemly arrayed Zf of the C
2H
2 variety. The remaining 3.2 kb of the cDNA comprises the 3'-untranslated region. PCR analysis detected the presence of pMLZ-4 RNA in liver, heart, kidney, spleen and brain of newborn mice. Hybridization of pMLZ-4 to genomic DNA from a number of species of vertebrates revealed the presence of homologous sequences only in mouse and rat. Unexpectedly, the probe also hybridized to a single band in yeast DNA digested with
EcoRI. NIH3T3 cells were stably transformed with a construct that over-expresses the pMLZ-4 mRNA. The stably transformed cells did not differ in appearance from untransformed cells, and an analysis of proteins from transformed and untransformed cells failed to detect any differences resulting from over-expression of the pMLZ-4 mRNA. |
| doi_str_mv | 10.1016/0378-1119(93)90395-J |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75623005</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><els_id>037811199390395J</els_id><sourcerecordid>16847609</sourcerecordid><originalsourceid>FETCH-LOGICAL-c417t-9be4b15e0a06c1b425aaaa0f08df1fcdf0f6b2437a396f230f35a853adfab3003</originalsourceid><addsrcrecordid>eNqFkUFvFDEMhSMEKkvhH4CUA0JwGHA2yczkUqlaWqCq4ALnyJNxaNBMUpLZSu2vJ8uu9gi--ODvWc_PjL0U8F6AaD-A7PpGCGHeGvnOgDS6uXrEVqLvTAMg-8dsdUSesmel_IJaWq9P2EmvlJJKrRhuphRD_MkxjtzdYEa3UA4PuIQUefIceUx3NPGHEF3jK0mZ3-a0UIgNRZfGndh9_HrOfU4zX26Iz2lbiNM98Yliec6eeJwKvTj0U_bj8uL75nNz_e3Tl835deOU6JbGDKQGoQkQWicGtdZYCzz0oxfejR58O6yV7FCa1q8leKmx1xJHj4Os956yN_u91d3vLZXFzqE4miaMVA3ZTrdVBfq_oGh71bVgKqj2oMuplEze3uYwY763AuzuBXaXr93la420f19gr6rs1WH_dphpPIoOmdf568Mci8PJZ4wulCOmWpBK9xU722NUQ7sLlG1xoSZOY8jkFjum8G8ffwCYhKLN</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16847609</pqid></control><display><type>article</type><title>Cloning and characterization of a novel zinc-finger protein-encoding cDNA from the mouse eye lens</title><source>Elsevier ScienceDirect Journals Complete - AutoHoldings</source><source>MEDLINE</source><creator>Brady, James P. ; Piatigorsky, Joram</creator><creatorcontrib>Brady, James P. ; Piatigorsky, Joram</creatorcontrib><description>Zinc fingers (Zf) are a common structural motif found in many nucleic acid-binding proteins. In an effort to identify potential transcription factors in the mouse eye lens, we have isolated a
Zf-containing clone from a newborn mouse lens cDNA library. The clone, named pMLZ-4, is 4.5 kb in length and contains an open reading frame of 1073 bp. The putative pMLZ-4 protein consists of a short, N-terminal acidic domain followed by twelve tandemly arrayed Zf of the C
2H
2 variety. The remaining 3.2 kb of the cDNA comprises the 3'-untranslated region. PCR analysis detected the presence of pMLZ-4 RNA in liver, heart, kidney, spleen and brain of newborn mice. Hybridization of pMLZ-4 to genomic DNA from a number of species of vertebrates revealed the presence of homologous sequences only in mouse and rat. Unexpectedly, the probe also hybridized to a single band in yeast DNA digested with
EcoRI. NIH3T3 cells were stably transformed with a construct that over-expresses the pMLZ-4 mRNA. The stably transformed cells did not differ in appearance from untransformed cells, and an analysis of proteins from transformed and untransformed cells failed to detect any differences resulting from over-expression of the pMLZ-4 mRNA.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(93)90395-J</identifier><identifier>PMID: 8444344</identifier><identifier>CODEN: GENED6</identifier><language>eng</language><publisher>Lausanne: Elsevier B.V</publisher><subject>3T3 Cells ; Amino Acid Sequence ; Animals ; Animals, Newborn ; Base Sequence ; Biological and medical sciences ; Biological Evolution ; Blotting, Northern ; Blotting, Southern ; Cloning, Molecular ; Conserved Sequence ; Crystallins - genetics ; DNA ; evolution ; expression construct ; Fundamental and applied biological sciences. Psychology ; Lens, Crystalline - metabolism ; Mice ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; Nucleic acid-binding domain ; Organ Specificity - genetics ; PCR ; Plasmids ; Polymerase Chain Reaction ; Transcription. Transcription factor. Splicing. Rna processing ; Zinc Fingers - genetics</subject><ispartof>Gene, 1993-02, Vol.124 (2), p.207-214</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-9be4b15e0a06c1b425aaaa0f08df1fcdf0f6b2437a396f230f35a853adfab3003</citedby><cites>FETCH-LOGICAL-c417t-9be4b15e0a06c1b425aaaa0f08df1fcdf0f6b2437a396f230f35a853adfab3003</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(93)90395-J$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3549,27923,27924,45994</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4603458$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8444344$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brady, James P.</creatorcontrib><creatorcontrib>Piatigorsky, Joram</creatorcontrib><title>Cloning and characterization of a novel zinc-finger protein-encoding cDNA from the mouse eye lens</title><title>Gene</title><addtitle>Gene</addtitle><description>Zinc fingers (Zf) are a common structural motif found in many nucleic acid-binding proteins. In an effort to identify potential transcription factors in the mouse eye lens, we have isolated a
Zf-containing clone from a newborn mouse lens cDNA library. The clone, named pMLZ-4, is 4.5 kb in length and contains an open reading frame of 1073 bp. The putative pMLZ-4 protein consists of a short, N-terminal acidic domain followed by twelve tandemly arrayed Zf of the C
2H
2 variety. The remaining 3.2 kb of the cDNA comprises the 3'-untranslated region. PCR analysis detected the presence of pMLZ-4 RNA in liver, heart, kidney, spleen and brain of newborn mice. Hybridization of pMLZ-4 to genomic DNA from a number of species of vertebrates revealed the presence of homologous sequences only in mouse and rat. Unexpectedly, the probe also hybridized to a single band in yeast DNA digested with
EcoRI. NIH3T3 cells were stably transformed with a construct that over-expresses the pMLZ-4 mRNA. The stably transformed cells did not differ in appearance from untransformed cells, and an analysis of proteins from transformed and untransformed cells failed to detect any differences resulting from over-expression of the pMLZ-4 mRNA.</description><subject>3T3 Cells</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Animals, Newborn</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>Blotting, Northern</subject><subject>Blotting, Southern</subject><subject>Cloning, Molecular</subject><subject>Conserved Sequence</subject><subject>Crystallins - genetics</subject><subject>DNA</subject><subject>evolution</subject><subject>expression construct</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Lens, Crystalline - metabolism</subject><subject>Mice</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Nucleic acid-binding domain</subject><subject>Organ Specificity - genetics</subject><subject>PCR</subject><subject>Plasmids</subject><subject>Polymerase Chain Reaction</subject><subject>Transcription. Transcription factor. Splicing. Rna processing</subject><subject>Zinc Fingers - genetics</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkUFvFDEMhSMEKkvhH4CUA0JwGHA2yczkUqlaWqCq4ALnyJNxaNBMUpLZSu2vJ8uu9gi--ODvWc_PjL0U8F6AaD-A7PpGCGHeGvnOgDS6uXrEVqLvTAMg-8dsdUSesmel_IJaWq9P2EmvlJJKrRhuphRD_MkxjtzdYEa3UA4PuIQUefIceUx3NPGHEF3jK0mZ3-a0UIgNRZfGndh9_HrOfU4zX26Iz2lbiNM98Yliec6eeJwKvTj0U_bj8uL75nNz_e3Tl835deOU6JbGDKQGoQkQWicGtdZYCzz0oxfejR58O6yV7FCa1q8leKmx1xJHj4Os956yN_u91d3vLZXFzqE4miaMVA3ZTrdVBfq_oGh71bVgKqj2oMuplEze3uYwY763AuzuBXaXr93la420f19gr6rs1WH_dphpPIoOmdf568Mci8PJZ4wulCOmWpBK9xU722NUQ7sLlG1xoSZOY8jkFjum8G8ffwCYhKLN</recordid><startdate>19930228</startdate><enddate>19930228</enddate><creator>Brady, James P.</creator><creator>Piatigorsky, Joram</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>7X8</scope></search><sort><creationdate>19930228</creationdate><title>Cloning and characterization of a novel zinc-finger protein-encoding cDNA from the mouse eye lens</title><author>Brady, James P. ; Piatigorsky, Joram</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-9be4b15e0a06c1b425aaaa0f08df1fcdf0f6b2437a396f230f35a853adfab3003</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>3T3 Cells</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Animals, Newborn</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological Evolution</topic><topic>Blotting, Northern</topic><topic>Blotting, Southern</topic><topic>Cloning, Molecular</topic><topic>Conserved Sequence</topic><topic>Crystallins - genetics</topic><topic>DNA</topic><topic>evolution</topic><topic>expression construct</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Lens, Crystalline - metabolism</topic><topic>Mice</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Nucleic acid-binding domain</topic><topic>Organ Specificity - genetics</topic><topic>PCR</topic><topic>Plasmids</topic><topic>Polymerase Chain Reaction</topic><topic>Transcription. Transcription factor. Splicing. Rna processing</topic><topic>Zinc Fingers - genetics</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brady, James P.</creatorcontrib><creatorcontrib>Piatigorsky, Joram</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brady, James P.</au><au>Piatigorsky, Joram</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of a novel zinc-finger protein-encoding cDNA from the mouse eye lens</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1993-02-28</date><risdate>1993</risdate><volume>124</volume><issue>2</issue><spage>207</spage><epage>214</epage><pages>207-214</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><coden>GENED6</coden><abstract>Zinc fingers (Zf) are a common structural motif found in many nucleic acid-binding proteins. In an effort to identify potential transcription factors in the mouse eye lens, we have isolated a
Zf-containing clone from a newborn mouse lens cDNA library. The clone, named pMLZ-4, is 4.5 kb in length and contains an open reading frame of 1073 bp. The putative pMLZ-4 protein consists of a short, N-terminal acidic domain followed by twelve tandemly arrayed Zf of the C
2H
2 variety. The remaining 3.2 kb of the cDNA comprises the 3'-untranslated region. PCR analysis detected the presence of pMLZ-4 RNA in liver, heart, kidney, spleen and brain of newborn mice. Hybridization of pMLZ-4 to genomic DNA from a number of species of vertebrates revealed the presence of homologous sequences only in mouse and rat. Unexpectedly, the probe also hybridized to a single band in yeast DNA digested with
EcoRI. NIH3T3 cells were stably transformed with a construct that over-expresses the pMLZ-4 mRNA. The stably transformed cells did not differ in appearance from untransformed cells, and an analysis of proteins from transformed and untransformed cells failed to detect any differences resulting from over-expression of the pMLZ-4 mRNA.</abstract><cop>Lausanne</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8444344</pmid><doi>10.1016/0378-1119(93)90395-J</doi><tpages>8</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0378-1119 |
| ispartof | Gene, 1993-02, Vol.124 (2), p.207-214 |
| issn | 0378-1119 1879-0038 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75623005 |
| source | Elsevier ScienceDirect Journals Complete - AutoHoldings; MEDLINE |
| subjects | 3T3 Cells Amino Acid Sequence Animals Animals, Newborn Base Sequence Biological and medical sciences Biological Evolution Blotting, Northern Blotting, Southern Cloning, Molecular Conserved Sequence Crystallins - genetics DNA evolution expression construct Fundamental and applied biological sciences. Psychology Lens, Crystalline - metabolism Mice Molecular and cellular biology Molecular genetics Molecular Sequence Data Nucleic acid-binding domain Organ Specificity - genetics PCR Plasmids Polymerase Chain Reaction Transcription. Transcription factor. Splicing. Rna processing Zinc Fingers - genetics |
| title | Cloning and characterization of a novel zinc-finger protein-encoding cDNA from the mouse eye lens |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-01-09T08%3A29%3A59IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Cloning%20and%20characterization%20of%20a%20novel%20zinc-finger%20protein-encoding%20cDNA%20from%20the%20mouse%20eye%20lens&rft.jtitle=Gene&rft.au=Brady,%20James%20P.&rft.date=1993-02-28&rft.volume=124&rft.issue=2&rft.spage=207&rft.epage=214&rft.pages=207-214&rft.issn=0378-1119&rft.eissn=1879-0038&rft.coden=GENED6&rft_id=info:doi/10.1016/0378-1119(93)90395-J&rft_dat=%3Cproquest_cross%3E16847609%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16847609&rft_id=info:pmid/8444344&rft_els_id=037811199390395J&rfr_iscdi=true |