Cloning and characterization of avocado fruit mRNAs and their expression during ripening and low-temperature storage
Differential screening of a cDNA library made from RNA extracted from avocado (Persea americana Mill cv. Hass) fruit stored at low temperature (7 degrees C) gave 23 cDNA clones grouped into 10 families, 6 of which showed increased expression during cold storage and normal ripening. Partial DNA seque...
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| Veröffentlicht in: | Plant molecular biology 1993-02, Vol.21 (3), p.437-449 |
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| creator | Dopico, B. (Nottingham Univ., Sutton Bonington, Loughborough (United Kingdom). Dept. of Physiology and Environmental Science) Lowe, A.L Wilson, I.D Merodio, C Grierson, D |
| description | Differential screening of a cDNA library made from RNA extracted from avocado (Persea americana Mill cv. Hass) fruit stored at low temperature (7 degrees C) gave 23 cDNA clones grouped into 10 families, 6 of which showed increased expression during cold storage and normal ripening. Partial DNA sequencing was carried out for representative clones. Database searches found homologies with a polygalacturonase (PG), endochitinase, cysteine proteinase inhibitor and several stress-related proteins. No homologies were detected for clones from six families and their biological role remains to be elucidated. A full-length cDNA sequence for avocado PG was obtained and the predicted amino acid sequence compared with those from other PGs. mRNA encoding PG increased markedly during normal ripening, slightly later than mRNAs for cellulase and ethylene-forming enzyme (EFE). Low-temperature storage delayed ripening and retarded the appearance of mRNAs for enzymes known to be involved in cell wall metabolism and ethylene synthesis, such as cellulase, PG and EFE, and also other mRNAs of unknown function. The removal of ethylene from the atmosphere surrounding stored fruit delayed the appearance of the mRNAs encoding cellulase and PG more than the cold storage itself, although it hardly affected the expression of the EFE mRNA or the accumulation of mRNAs homologous to some other unidentified clones. |
| doi_str_mv | 10.1007/bf00028802 |
| format | Article |
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(Nottingham Univ., Sutton Bonington, Loughborough (United Kingdom). Dept. of Physiology and Environmental Science) ; Lowe, A.L ; Wilson, I.D ; Merodio, C ; Grierson, D</creator><creatorcontrib>Dopico, B. (Nottingham Univ., Sutton Bonington, Loughborough (United Kingdom). Dept. of Physiology and Environmental Science) ; Lowe, A.L ; Wilson, I.D ; Merodio, C ; Grierson, D</creatorcontrib><description>Differential screening of a cDNA library made from RNA extracted from avocado (Persea americana Mill cv. Hass) fruit stored at low temperature (7 degrees C) gave 23 cDNA clones grouped into 10 families, 6 of which showed increased expression during cold storage and normal ripening. Partial DNA sequencing was carried out for representative clones. Database searches found homologies with a polygalacturonase (PG), endochitinase, cysteine proteinase inhibitor and several stress-related proteins. No homologies were detected for clones from six families and their biological role remains to be elucidated. A full-length cDNA sequence for avocado PG was obtained and the predicted amino acid sequence compared with those from other PGs. mRNA encoding PG increased markedly during normal ripening, slightly later than mRNAs for cellulase and ethylene-forming enzyme (EFE). Low-temperature storage delayed ripening and retarded the appearance of mRNAs for enzymes known to be involved in cell wall metabolism and ethylene synthesis, such as cellulase, PG and EFE, and also other mRNAs of unknown function. The removal of ethylene from the atmosphere surrounding stored fruit delayed the appearance of the mRNAs encoding cellulase and PG more than the cold storage itself, although it hardly affected the expression of the EFE mRNA or the accumulation of mRNAs homologous to some other unidentified clones.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/bf00028802</identifier><identifier>PMID: 8095163</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>ADN ; ALMACENAMIENTO ; Amino Acid Sequence ; ARN MENSAJERO ; ARN MESSAGER ; Base Sequence ; Biological and medical sciences ; CLONACION ; CLONAGE ; CLONING ; Cloning, Molecular ; DNA ; Ethylenes - pharmacology ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fruit - enzymology ; Fruit - genetics ; Fruit - growth & development ; Fundamental and applied biological sciences. 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(Nottingham Univ., Sutton Bonington, Loughborough (United Kingdom). Dept. of Physiology and Environmental Science)</creatorcontrib><creatorcontrib>Lowe, A.L</creatorcontrib><creatorcontrib>Wilson, I.D</creatorcontrib><creatorcontrib>Merodio, C</creatorcontrib><creatorcontrib>Grierson, D</creatorcontrib><title>Cloning and characterization of avocado fruit mRNAs and their expression during ripening and low-temperature storage</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>Differential screening of a cDNA library made from RNA extracted from avocado (Persea americana Mill cv. Hass) fruit stored at low temperature (7 degrees C) gave 23 cDNA clones grouped into 10 families, 6 of which showed increased expression during cold storage and normal ripening. Partial DNA sequencing was carried out for representative clones. Database searches found homologies with a polygalacturonase (PG), endochitinase, cysteine proteinase inhibitor and several stress-related proteins. No homologies were detected for clones from six families and their biological role remains to be elucidated. A full-length cDNA sequence for avocado PG was obtained and the predicted amino acid sequence compared with those from other PGs. mRNA encoding PG increased markedly during normal ripening, slightly later than mRNAs for cellulase and ethylene-forming enzyme (EFE). Low-temperature storage delayed ripening and retarded the appearance of mRNAs for enzymes known to be involved in cell wall metabolism and ethylene synthesis, such as cellulase, PG and EFE, and also other mRNAs of unknown function. The removal of ethylene from the atmosphere surrounding stored fruit delayed the appearance of the mRNAs encoding cellulase and PG more than the cold storage itself, although it hardly affected the expression of the EFE mRNA or the accumulation of mRNAs homologous to some other unidentified clones.</description><subject>ADN</subject><subject>ALMACENAMIENTO</subject><subject>Amino Acid Sequence</subject><subject>ARN MENSAJERO</subject><subject>ARN MESSAGER</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>CLONACION</subject><subject>CLONAGE</subject><subject>CLONING</subject><subject>Cloning, Molecular</subject><subject>DNA</subject><subject>Ethylenes - pharmacology</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fruit - enzymology</subject><subject>Fruit - genetics</subject><subject>Fruit - growth & development</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE EXPRESSION</subject><subject>Gene Expression Regulation, Enzymologic - drug effects</subject><subject>MADURAMIENTO</subject><subject>MESSENGER RNA</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>MURISSAGE</subject><subject>PERSEA AMERICANA</subject><subject>POLIGALACTURONASA</subject><subject>Poly A - metabolism</subject><subject>POLYGALACTURONASE</subject><subject>Polygalacturonase - genetics</subject><subject>Refrigeration</subject><subject>RIPENING</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><subject>STOCKAGE</subject><subject>STORAGE</subject><subject>TEMPERATURA</subject><subject>TEMPERATURE</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0c-r1DAQB_AgynN9evEoCD2IB6GaZJqkPb63-FRYniB6LtN0si_SNjVJ_fXX23XXvXoahvnwHZhh7KngrwXn5k3nOOeyrrm8xzZCGSjV2t5nGy60KatKyIfsUUpfOV856At2UfNGCQ0blrdDmPy0L3DqC3uHEW2m6H9j9mEqgivwe7DYh8LFxedi_HR7lf7afEc-FvRzjpTSwfZLPOREP9M5cAg_ykzjTBHzEqlIOUTc02P2wOGQ6MmpXrIvN28_b9-Xu4_vPmyvdqUFrnJJjYCqqa2ujDMSjSEQBFXf9LIWprbQgYKGuw7R6p76DrgzYEBZI6XoJVyyl8fcOYZvC6Xcjj5ZGgacKCypNUoLpZX-LxRagVS6WuGrI7QxpBTJtXP0I8ZfreDt4Rft9c2_X6z4-Sl16Ubqz_R0_HX-4jTHZHFwESfr05lVGhpdHXY-OzKHocV9XMntrgEujGrgD3NMmYw</recordid><startdate>199302</startdate><enddate>199302</enddate><creator>Dopico, B. (Nottingham Univ., Sutton Bonington, Loughborough (United Kingdom). Dept. of Physiology and Environmental Science)</creator><creator>Lowe, A.L</creator><creator>Wilson, I.D</creator><creator>Merodio, C</creator><creator>Grierson, D</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199302</creationdate><title>Cloning and characterization of avocado fruit mRNAs and their expression during ripening and low-temperature storage</title><author>Dopico, B. (Nottingham Univ., Sutton Bonington, Loughborough (United Kingdom). Dept. of Physiology and Environmental Science) ; Lowe, A.L ; Wilson, I.D ; Merodio, C ; Grierson, D</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c305t-e913498c647f72a77e31e34d9d28178c3b35390fbaac6dedb30f73735c7221d23</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>ADN</topic><topic>ALMACENAMIENTO</topic><topic>Amino Acid Sequence</topic><topic>ARN MENSAJERO</topic><topic>ARN MESSAGER</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>CLONACION</topic><topic>CLONAGE</topic><topic>CLONING</topic><topic>Cloning, Molecular</topic><topic>DNA</topic><topic>Ethylenes - pharmacology</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fruit - enzymology</topic><topic>Fruit - genetics</topic><topic>Fruit - growth & development</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE EXPRESSION</topic><topic>Gene Expression Regulation, Enzymologic - drug effects</topic><topic>MADURAMIENTO</topic><topic>MESSENGER RNA</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>MURISSAGE</topic><topic>PERSEA AMERICANA</topic><topic>POLIGALACTURONASA</topic><topic>Poly A - metabolism</topic><topic>POLYGALACTURONASE</topic><topic>Polygalacturonase - genetics</topic><topic>Refrigeration</topic><topic>RIPENING</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><topic>STOCKAGE</topic><topic>STORAGE</topic><topic>TEMPERATURA</topic><topic>TEMPERATURE</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Dopico, B. (Nottingham Univ., Sutton Bonington, Loughborough (United Kingdom). Dept. of Physiology and Environmental Science)</creatorcontrib><creatorcontrib>Lowe, A.L</creatorcontrib><creatorcontrib>Wilson, I.D</creatorcontrib><creatorcontrib>Merodio, C</creatorcontrib><creatorcontrib>Grierson, D</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Dopico, B. (Nottingham Univ., Sutton Bonington, Loughborough (United Kingdom). Dept. of Physiology and Environmental Science)</au><au>Lowe, A.L</au><au>Wilson, I.D</au><au>Merodio, C</au><au>Grierson, D</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Cloning and characterization of avocado fruit mRNAs and their expression during ripening and low-temperature storage</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1993-02</date><risdate>1993</risdate><volume>21</volume><issue>3</issue><spage>437</spage><epage>449</epage><pages>437-449</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>Differential screening of a cDNA library made from RNA extracted from avocado (Persea americana Mill cv. Hass) fruit stored at low temperature (7 degrees C) gave 23 cDNA clones grouped into 10 families, 6 of which showed increased expression during cold storage and normal ripening. Partial DNA sequencing was carried out for representative clones. Database searches found homologies with a polygalacturonase (PG), endochitinase, cysteine proteinase inhibitor and several stress-related proteins. No homologies were detected for clones from six families and their biological role remains to be elucidated. A full-length cDNA sequence for avocado PG was obtained and the predicted amino acid sequence compared with those from other PGs. mRNA encoding PG increased markedly during normal ripening, slightly later than mRNAs for cellulase and ethylene-forming enzyme (EFE). Low-temperature storage delayed ripening and retarded the appearance of mRNAs for enzymes known to be involved in cell wall metabolism and ethylene synthesis, such as cellulase, PG and EFE, and also other mRNAs of unknown function. The removal of ethylene from the atmosphere surrounding stored fruit delayed the appearance of the mRNAs encoding cellulase and PG more than the cold storage itself, although it hardly affected the expression of the EFE mRNA or the accumulation of mRNAs homologous to some other unidentified clones.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>8095163</pmid><doi>10.1007/bf00028802</doi><tpages>13</tpages></addata></record> |
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| ispartof | Plant molecular biology, 1993-02, Vol.21 (3), p.437-449 |
| issn | 0167-4412 1573-5028 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75615656 |
| source | MEDLINE; SpringerLink Journals - AutoHoldings |
| subjects | ADN ALMACENAMIENTO Amino Acid Sequence ARN MENSAJERO ARN MESSAGER Base Sequence Biological and medical sciences CLONACION CLONAGE CLONING Cloning, Molecular DNA Ethylenes - pharmacology EXPRESION GENICA EXPRESSION DES GENES Fruit - enzymology Fruit - genetics Fruit - growth & development Fundamental and applied biological sciences. Psychology GENE EXPRESSION Gene Expression Regulation, Enzymologic - drug effects MADURAMIENTO MESSENGER RNA Molecular and cellular biology Molecular genetics Molecular Sequence Data MURISSAGE PERSEA AMERICANA POLIGALACTURONASA Poly A - metabolism POLYGALACTURONASE Polygalacturonase - genetics Refrigeration RIPENING RNA, Messenger - metabolism Sequence Homology, Amino Acid STOCKAGE STORAGE TEMPERATURA TEMPERATURE |
| title | Cloning and characterization of avocado fruit mRNAs and their expression during ripening and low-temperature storage |
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