Inactivation of interleukin-8 by the C5a-inactivating protease from serosal fluid
The complement fragment C5a and the cytokine interleukin-8 (IL-8) are proinflammatory peptides with potent chemotactic activity toward neutrophils. We have previously shown that C5a can be inactivated by a protease that is found in normal synovial and peritoneal fluids but is absent from serosal flu...
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| Veröffentlicht in: | Blood 1993-03, Vol.81 (6), p.1424-1427 |
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| creator | AYESH, S. K AZAR, Y BABIOR, B. M MATZNER, Y |
| description | The complement fragment C5a and the cytokine interleukin-8 (IL-8) are proinflammatory peptides with potent chemotactic activity toward neutrophils. We have previously shown that C5a can be inactivated by a protease that is found in normal synovial and peritoneal fluids but is absent from serosal fluids obtained from patients with familial Mediterranean fever (FMF). We report here that serosal fluids can also eliminate the chemotactic activity of IL-8. The agent responsible for IL-8 elimination appears to be the C5a-inactivating protease, because the pure protease can inactivate IL-8, inactivation of IL-8 by normal peritoneal fluid is partly prevented by an antibody raised against the purified C5a-inactivating protease, and IL-8 is not inactivated by peritoneal fluids from patients with FMF. The ability of this protease to inactivate both, early (C5a) and late (IL-8) inflammatory mediators identifies it as a potentially significant regulator of inflammation. |
| doi_str_mv | 10.1182/blood.V81.6.1424.1424 |
| format | Article |
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The ability of this protease to inactivate both, early (C5a) and late (IL-8) inflammatory mediators identifies it as a potentially significant regulator of inflammation.</description><identifier>ISSN: 0006-4971</identifier><identifier>EISSN: 1528-0020</identifier><identifier>DOI: 10.1182/blood.V81.6.1424.1424</identifier><identifier>PMID: 8453091</identifier><language>eng</language><publisher>Washington, DC: The Americain Society of Hematology</publisher><subject>Analysis of the immune response. Humoral and cellular immunity ; Ascitic Fluid - enzymology ; Biological and medical sciences ; Chemotaxis, Leukocyte ; Complement C5a - antagonists & inhibitors ; Fundamental and applied biological sciences. 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The agent responsible for IL-8 elimination appears to be the C5a-inactivating protease, because the pure protease can inactivate IL-8, inactivation of IL-8 by normal peritoneal fluid is partly prevented by an antibody raised against the purified C5a-inactivating protease, and IL-8 is not inactivated by peritoneal fluids from patients with FMF. The ability of this protease to inactivate both, early (C5a) and late (IL-8) inflammatory mediators identifies it as a potentially significant regulator of inflammation.</description><subject>Analysis of the immune response. Humoral and cellular immunity</subject><subject>Ascitic Fluid - enzymology</subject><subject>Biological and medical sciences</subject><subject>Chemotaxis, Leukocyte</subject><subject>Complement C5a - antagonists & inhibitors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>Humans</subject><subject>Immunobiology</subject><subject>Inflammation - etiology</subject><subject>Interleukin-8 - antagonists & inhibitors</subject><subject>Lymphokines, interleukins ( function, expression)</subject><subject>Neutrophils</subject><subject>Regulatory factors and their cellular receptors</subject><subject>Serine Endopeptidases - metabolism</subject><issn>0006-4971</issn><issn>1528-0020</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kFtLxDAQhYMo67r6ExbyIL61TppLk0dZvCwsiKC-hrSZarTbatMK--_tXtiXmYFzZubwETJnkDKms9uiblufvmuWqpSJTOzKCZkymekEIINTMgUAlQiTs3NyEeMXABM8kxMy0UJyMGxKXpaNK_vw5_rQNrStaGh67GocvkOTaFpsaP-JdCFdEo7G5oP-dG2PLiKtunZNI3ZtdDWt6iH4S3JWuTri1aHPyNvD_eviKVk9Py4Xd6uk5Br6hHOZ84JrYZySXmnvJXoOmcwNM1IAQ81VJQVDjrIwWudOS-5R-RzyfBxn5GZ_d8zyO2Ds7TrEEuvaNdgO0eZSgcmEGY1ybyzHlLHDyv50Ye26jWVgtyjtDqUdUVpltxR3ZdybHx4MxRr9cevAbtSvD7qLpaurzjVliEebUAb4mPMfnnl8dg</recordid><startdate>19930315</startdate><enddate>19930315</enddate><creator>AYESH, S. 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Humoral and cellular immunity</topic><topic>Ascitic Fluid - enzymology</topic><topic>Biological and medical sciences</topic><topic>Chemotaxis, Leukocyte</topic><topic>Complement C5a - antagonists & inhibitors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>Humans</topic><topic>Immunobiology</topic><topic>Inflammation - etiology</topic><topic>Interleukin-8 - antagonists & inhibitors</topic><topic>Lymphokines, interleukins ( function, expression)</topic><topic>Neutrophils</topic><topic>Regulatory factors and their cellular receptors</topic><topic>Serine Endopeptidases - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>AYESH, S. K</creatorcontrib><creatorcontrib>AZAR, Y</creatorcontrib><creatorcontrib>BABIOR, B. 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The agent responsible for IL-8 elimination appears to be the C5a-inactivating protease, because the pure protease can inactivate IL-8, inactivation of IL-8 by normal peritoneal fluid is partly prevented by an antibody raised against the purified C5a-inactivating protease, and IL-8 is not inactivated by peritoneal fluids from patients with FMF. The ability of this protease to inactivate both, early (C5a) and late (IL-8) inflammatory mediators identifies it as a potentially significant regulator of inflammation.</abstract><cop>Washington, DC</cop><pub>The Americain Society of Hematology</pub><pmid>8453091</pmid><doi>10.1182/blood.V81.6.1424.1424</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | Blood, 1993-03, Vol.81 (6), p.1424-1427 |
| issn | 0006-4971 1528-0020 |
| language | eng |
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| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Analysis of the immune response. Humoral and cellular immunity Ascitic Fluid - enzymology Biological and medical sciences Chemotaxis, Leukocyte Complement C5a - antagonists & inhibitors Fundamental and applied biological sciences. Psychology Fundamental immunology Humans Immunobiology Inflammation - etiology Interleukin-8 - antagonists & inhibitors Lymphokines, interleukins ( function, expression) Neutrophils Regulatory factors and their cellular receptors Serine Endopeptidases - metabolism |
| title | Inactivation of interleukin-8 by the C5a-inactivating protease from serosal fluid |
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