Immunoradiometric assay of human leukocyte interferon using monoclonal antibody
Interferon research has been hampered by problems in its assay. The only widespread assays use tissue culture cells and compare some parameter of viral growth (such as viral RNA synthesis or host cell death) in the presence and absence of interferon 1 . These complex biological assays, though sensit...
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| Veröffentlicht in: | Nature (London) 1981-04, Vol.290 (5806), p.501-503 |
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| creator | Secher, D. S. |
| description | Interferon research has been hampered by problems in its assay. The only widespread assays use tissue culture cells and compare some parameter of viral growth (such as viral RNA synthesis or host cell death) in the presence and absence of interferon
1
. These complex biological assays, though sensitive, are laborious and subject to inherent variability. In particular, components other than interferon present in the assay sample often influence viral growth. There is clearly a need for a simple, direct, interferon assay; I now describe such an assay—an immunoradiometric assay utilizing monoclonal antibody. |
| doi_str_mv | 10.1038/290501a0 |
| format | Article |
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. These complex biological assays, though sensitive, are laborious and subject to inherent variability. In particular, components other than interferon present in the assay sample often influence viral growth. There is clearly a need for a simple, direct, interferon assay; I now describe such an assay—an immunoradiometric assay utilizing monoclonal antibody.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/290501a0</identifier><identifier>PMID: 6163989</identifier><language>eng</language><publisher>London: Nature Publishing Group UK</publisher><subject>Antibodies - analysis ; Antibodies, Monoclonal ; Antibody Specificity ; Clone Cells - immunology ; Humanities and Social Sciences ; Humans ; Hybrid Cells - immunology ; Interferons - analysis ; letter ; multidisciplinary ; Radioimmunoassay - methods ; Science ; Science (multidisciplinary)</subject><ispartof>Nature (London), 1981-04, Vol.290 (5806), p.501-503</ispartof><rights>Springer Nature Limited 1981</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c312t-e4a48e91c60cdb43fd9135c7e91fb4ca8d38f2ae300380c1344fa38414c73ee93</citedby><cites>FETCH-LOGICAL-c312t-e4a48e91c60cdb43fd9135c7e91fb4ca8d38f2ae300380c1344fa38414c73ee93</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://link.springer.com/content/pdf/10.1038/290501a0$$EPDF$$P50$$Gspringer$$H</linktopdf><linktohtml>$$Uhttps://link.springer.com/10.1038/290501a0$$EHTML$$P50$$Gspringer$$H</linktohtml><link.rule.ids>314,776,780,27901,27902,41464,42533,51294</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6163989$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Secher, D. S.</creatorcontrib><title>Immunoradiometric assay of human leukocyte interferon using monoclonal antibody</title><title>Nature (London)</title><addtitle>Nature</addtitle><addtitle>Nature</addtitle><description>Interferon research has been hampered by problems in its assay. The only widespread assays use tissue culture cells and compare some parameter of viral growth (such as viral RNA synthesis or host cell death) in the presence and absence of interferon
1
. These complex biological assays, though sensitive, are laborious and subject to inherent variability. In particular, components other than interferon present in the assay sample often influence viral growth. There is clearly a need for a simple, direct, interferon assay; I now describe such an assay—an immunoradiometric assay utilizing monoclonal antibody.</description><subject>Antibodies - analysis</subject><subject>Antibodies, Monoclonal</subject><subject>Antibody Specificity</subject><subject>Clone Cells - immunology</subject><subject>Humanities and Social Sciences</subject><subject>Humans</subject><subject>Hybrid Cells - immunology</subject><subject>Interferons - analysis</subject><subject>letter</subject><subject>multidisciplinary</subject><subject>Radioimmunoassay - methods</subject><subject>Science</subject><subject>Science (multidisciplinary)</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkE1LxDAURYMo4zgK_gGhK9FF9aVJ23Qpgx8DA7PRdUnTl7Fjk2jSLPrvrczoxtWDdw8X7iHkksIdBSbuswpyoBKOyJzyskh5IcpjMgfIRAqCFafkLIQdAOS05DMyK2jBKlHNyWZlTLTOy7ZzBgffqUSGIMfE6eQ9GmmTHuOHU-OASWcH9Bq9s0kMnd0mxlmnemdln0g7dI1rx3NyomUf8OJwF-Tt6fF1-ZKuN8-r5cM6VYxmQ4pccoEVVQWotuFMtxVluSqnl264kqJlQmcSGUzrQFHGuZZMcMpVyRArtiDX-95P774ihqE2XVDY99Kii6Eu8wJKwdkE3uxB5V0IHnX96Tsj_VhTqH_c1b_uJvTq0Bkbg-0feJA15bf7PEyJ3aKvdy76aX343_UNpDh3fg</recordid><startdate>19810409</startdate><enddate>19810409</enddate><creator>Secher, D. S.</creator><general>Nature Publishing Group UK</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19810409</creationdate><title>Immunoradiometric assay of human leukocyte interferon using monoclonal antibody</title><author>Secher, D. S.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c312t-e4a48e91c60cdb43fd9135c7e91fb4ca8d38f2ae300380c1344fa38414c73ee93</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Antibodies - analysis</topic><topic>Antibodies, Monoclonal</topic><topic>Antibody Specificity</topic><topic>Clone Cells - immunology</topic><topic>Humanities and Social Sciences</topic><topic>Humans</topic><topic>Hybrid Cells - immunology</topic><topic>Interferons - analysis</topic><topic>letter</topic><topic>multidisciplinary</topic><topic>Radioimmunoassay - methods</topic><topic>Science</topic><topic>Science (multidisciplinary)</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Secher, D. S.</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Nature (London)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Secher, D. S.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Immunoradiometric assay of human leukocyte interferon using monoclonal antibody</atitle><jtitle>Nature (London)</jtitle><stitle>Nature</stitle><addtitle>Nature</addtitle><date>1981-04-09</date><risdate>1981</risdate><volume>290</volume><issue>5806</issue><spage>501</spage><epage>503</epage><pages>501-503</pages><issn>0028-0836</issn><eissn>1476-4687</eissn><abstract>Interferon research has been hampered by problems in its assay. The only widespread assays use tissue culture cells and compare some parameter of viral growth (such as viral RNA synthesis or host cell death) in the presence and absence of interferon
1
. These complex biological assays, though sensitive, are laborious and subject to inherent variability. In particular, components other than interferon present in the assay sample often influence viral growth. There is clearly a need for a simple, direct, interferon assay; I now describe such an assay—an immunoradiometric assay utilizing monoclonal antibody.</abstract><cop>London</cop><pub>Nature Publishing Group UK</pub><pmid>6163989</pmid><doi>10.1038/290501a0</doi><tpages>3</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1981-04, Vol.290 (5806), p.501-503 |
| issn | 0028-0836 1476-4687 |
| language | eng |
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| source | MEDLINE; Springer Nature - Complete Springer Journals; Nature Journals Online |
| subjects | Antibodies - analysis Antibodies, Monoclonal Antibody Specificity Clone Cells - immunology Humanities and Social Sciences Humans Hybrid Cells - immunology Interferons - analysis letter multidisciplinary Radioimmunoassay - methods Science Science (multidisciplinary) |
| title | Immunoradiometric assay of human leukocyte interferon using monoclonal antibody |
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