Persistence of initial infection in recurrent Cryptococcus neoformans meningitis
Patients with cryptococcal meningitis tend to have recurrences of infection. Although the original strain of Cryptococcus neoformans is assumed to persist in recurrent infections, this assumption has not been tested. Southern blot hybridisation with two genomic DNA probes and pulsed-field electropho...
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| Veröffentlicht in: | The Lancet (British edition) 1993-03, Vol.341 (8845), p.595-596 |
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| creator | Spitzer, E.D. Spitzer, S.G. Freundlich, L.F. Casadevall, A. |
| description | Patients with cryptococcal meningitis tend to have recurrences of infection. Although the original strain of
Cryptococcus neoformans is assumed to persist in recurrent infections, this assumption has not been tested. Southern blot hybridisation with two genomic DNA probes and pulsed-field electrophoresis of intact chromosomes were used to investigate the genetic relation between initial and relapse isolates of
C neoformans from patients with recurrent cryptococcal meningitis. Eleven isolates were obtained from four patients (three with AIDS, one with leukaemia). Isolates from each patient could be distinguished from those of the other patients; however, each patient's initial and recurrence isolates were clonally related. Our results provide strong evidence that clinical recurrences of cryptococcal meningitis result from persistence of the original infecting strain. |
| doi_str_mv | 10.1016/0140-6736(93)90354-J |
| format | Article |
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Cryptococcus neoformans is assumed to persist in recurrent infections, this assumption has not been tested. Southern blot hybridisation with two genomic DNA probes and pulsed-field electrophoresis of intact chromosomes were used to investigate the genetic relation between initial and relapse isolates of
C neoformans from patients with recurrent cryptococcal meningitis. Eleven isolates were obtained from four patients (three with AIDS, one with leukaemia). Isolates from each patient could be distinguished from those of the other patients; however, each patient's initial and recurrence isolates were clonally related. Our results provide strong evidence that clinical recurrences of cryptococcal meningitis result from persistence of the original infecting strain.</description><identifier>ISSN: 0140-6736</identifier><identifier>EISSN: 1474-547X</identifier><identifier>DOI: 10.1016/0140-6736(93)90354-J</identifier><identifier>PMID: 8094831</identifier><identifier>CODEN: LANCAO</identifier><language>eng</language><publisher>London: Elsevier Ltd</publisher><subject>Acquired Immunodeficiency Syndrome - complications ; Adult ; Aged ; AIDS/HIV ; Biological and medical sciences ; Blotting, Southern - standards ; Cerebrospinal Fluid - microbiology ; Chromosomes ; Deoxyribonucleic acid ; Diagnosis, Differential ; DNA ; Electrophoresis ; Electrophoresis, Gel, Pulsed-Field - standards ; Fluconazole - therapeutic use ; Genetics ; Human mycoses ; Humans ; Infections ; Infectious diseases ; Karyotyping ; Leukemia ; Leukemia, Lymphocytic, Chronic, B-Cell - complications ; Male ; Medical research ; Medical sciences ; Meningitis ; Meningitis, Cryptococcal - drug therapy ; Meningitis, Cryptococcal - epidemiology ; Meningitis, Cryptococcal - microbiology ; Mycoses ; Mycoses of the nervous system ; Polymorphism, Restriction Fragment Length ; Probes ; Recurrence ; Sensitivity and Specificity</subject><ispartof>The Lancet (British edition), 1993-03, Vol.341 (8845), p.595-596</ispartof><rights>1993</rights><rights>1993 INIST-CNRS</rights><rights>Copyright Lancet Ltd. Mar 6, 1993</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c413t-3a05fdf754542f6f8c0251d224db6ebb3478cffd975935114d4681979e5193d63</citedby><cites>FETCH-LOGICAL-c413t-3a05fdf754542f6f8c0251d224db6ebb3478cffd975935114d4681979e5193d63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.proquest.com/docview/199015146?pq-origsite=primo$$EHTML$$P50$$Gproquest$$H</linktohtml><link.rule.ids>314,777,781,3537,27905,27906,45976,64364,64366,64368,72218</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4653696$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8094831$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Spitzer, E.D.</creatorcontrib><creatorcontrib>Spitzer, S.G.</creatorcontrib><creatorcontrib>Freundlich, L.F.</creatorcontrib><creatorcontrib>Casadevall, A.</creatorcontrib><title>Persistence of initial infection in recurrent Cryptococcus neoformans meningitis</title><title>The Lancet (British edition)</title><addtitle>Lancet</addtitle><description>Patients with cryptococcal meningitis tend to have recurrences of infection. Although the original strain of
Cryptococcus neoformans is assumed to persist in recurrent infections, this assumption has not been tested. Southern blot hybridisation with two genomic DNA probes and pulsed-field electrophoresis of intact chromosomes were used to investigate the genetic relation between initial and relapse isolates of
C neoformans from patients with recurrent cryptococcal meningitis. Eleven isolates were obtained from four patients (three with AIDS, one with leukaemia). Isolates from each patient could be distinguished from those of the other patients; however, each patient's initial and recurrence isolates were clonally related. Our results provide strong evidence that clinical recurrences of cryptococcal meningitis result from persistence of the original infecting strain.</description><subject>Acquired Immunodeficiency Syndrome - complications</subject><subject>Adult</subject><subject>Aged</subject><subject>AIDS/HIV</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern - standards</subject><subject>Cerebrospinal Fluid - microbiology</subject><subject>Chromosomes</subject><subject>Deoxyribonucleic acid</subject><subject>Diagnosis, Differential</subject><subject>DNA</subject><subject>Electrophoresis</subject><subject>Electrophoresis, Gel, Pulsed-Field - standards</subject><subject>Fluconazole - therapeutic use</subject><subject>Genetics</subject><subject>Human mycoses</subject><subject>Humans</subject><subject>Infections</subject><subject>Infectious diseases</subject><subject>Karyotyping</subject><subject>Leukemia</subject><subject>Leukemia, Lymphocytic, Chronic, B-Cell - complications</subject><subject>Male</subject><subject>Medical research</subject><subject>Medical sciences</subject><subject>Meningitis</subject><subject>Meningitis, Cryptococcal - drug therapy</subject><subject>Meningitis, Cryptococcal - epidemiology</subject><subject>Meningitis, Cryptococcal - microbiology</subject><subject>Mycoses</subject><subject>Mycoses of the nervous system</subject><subject>Polymorphism, Restriction Fragment Length</subject><subject>Probes</subject><subject>Recurrence</subject><subject>Sensitivity and 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meningitis tend to have recurrences of infection. Although the original strain of
Cryptococcus neoformans is assumed to persist in recurrent infections, this assumption has not been tested. Southern blot hybridisation with two genomic DNA probes and pulsed-field electrophoresis of intact chromosomes were used to investigate the genetic relation between initial and relapse isolates of
C neoformans from patients with recurrent cryptococcal meningitis. Eleven isolates were obtained from four patients (three with AIDS, one with leukaemia). Isolates from each patient could be distinguished from those of the other patients; however, each patient's initial and recurrence isolates were clonally related. Our results provide strong evidence that clinical recurrences of cryptococcal meningitis result from persistence of the original infecting strain.</abstract><cop>London</cop><pub>Elsevier Ltd</pub><pmid>8094831</pmid><doi>10.1016/0140-6736(93)90354-J</doi><tpages>2</tpages></addata></record> |
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| ispartof | The Lancet (British edition), 1993-03, Vol.341 (8845), p.595-596 |
| issn | 0140-6736 1474-547X |
| language | eng |
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| subjects | Acquired Immunodeficiency Syndrome - complications Adult Aged AIDS/HIV Biological and medical sciences Blotting, Southern - standards Cerebrospinal Fluid - microbiology Chromosomes Deoxyribonucleic acid Diagnosis, Differential DNA Electrophoresis Electrophoresis, Gel, Pulsed-Field - standards Fluconazole - therapeutic use Genetics Human mycoses Humans Infections Infectious diseases Karyotyping Leukemia Leukemia, Lymphocytic, Chronic, B-Cell - complications Male Medical research Medical sciences Meningitis Meningitis, Cryptococcal - drug therapy Meningitis, Cryptococcal - epidemiology Meningitis, Cryptococcal - microbiology Mycoses Mycoses of the nervous system Polymorphism, Restriction Fragment Length Probes Recurrence Sensitivity and Specificity |
| title | Persistence of initial infection in recurrent Cryptococcus neoformans meningitis |
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