High subretinal fluid procoagulant activity in rhegmatogenous retinal detachment
An increased mRNA expression of genes related to blood coagulation has been demonstrated in an experimental retinal detachment model but has not yet been confirmed in human clinical specimens. Tissue factor (TF), the initiating factor of blood coagulation, may be a determinant of the extent of tissu...
Gespeichert in:
| Veröffentlicht in: | Investigative ophthalmology & visual science 2010-10, Vol.51 (10), p.5234-5239 |
|---|---|
| Hauptverfasser: | , , , , , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 5239 |
|---|---|
| container_issue | 10 |
| container_start_page | 5234 |
| container_title | Investigative ophthalmology & visual science |
| container_volume | 51 |
| creator | Ricker, Lukas J A G Dieri, Raed Al Beckers, Gerold J M Pels, Elisabeth Liem, Albert T A Hendrikse, Fred Kijlstra, Aize Hemker, H Coenraad La Heij, Ellen C |
| description | An increased mRNA expression of genes related to blood coagulation has been demonstrated in an experimental retinal detachment model but has not yet been confirmed in human clinical specimens. Tissue factor (TF), the initiating factor of blood coagulation, may be a determinant of the extent of tissue injury after rhegmatogenous retinal detachment (RRD). This study was conducted to determine whether subretinal fluid and vitreous fluid collected from patients with RRD have a procoagulant effect.
Calibrated thrombin generation (CAT) was used to investigate the thrombogenic properties of 28 subretinal fluids collected during scleral buckling surgery for RRD. Further, the thrombogenic properties of vitreous fluids from RRD (n = 12), macular pucker (n = 5), macular hole (n = 6), and proliferative diabetic retinopathy (n = 5) were compared with the properties of eye bank eyes (n = 11), which served as control specimens. The procoagulant activity of TF was determined with Western blot analysis.
The addition of subretinal fluid from all RRD patients (28/28, 100%) induced thrombin generation in normal and severely factor (F)XII-deficient plasma. Contrary to the subretinal fluid, the addition of vitreous fluids from various ocular disorders evoked very little thrombin generation in normal and severely FXII-deficient plasma (4/12, 33% RRD; 1/5, 20% macular pucker; 0/6, 0% macular hole; 0/5, 0% proliferative diabetic retinopathy; and 2/11, 18% eye bank eyes). The procoagulant activity in subretinal fluid was almost completely neutralized by antibodies against human TF. The presence of TF in subretinal fluid was confirmed by Western blot.
Subretinal fluid of patients with RRD induces high procoagulant activity, determined by measuring the level of tissue factor. |
| doi_str_mv | 10.1167/iovs.10-5354 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_755970900</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>755970900</sourcerecordid><originalsourceid>FETCH-LOGICAL-c290t-7b6a5348dd06398592b9d384559ba224eb55ad7b39b9564b4823cb1e237d5d73</originalsourceid><addsrcrecordid>eNpNkE1LAzEQhoMotlZvnmVvXtyaj51NcpSiVijoofcl2aTbyH7UJFvov3eXtuJpZuCZl5cHoXuC54Tk_Nl1-zAnOAUG2QWaEgCaAhfs8t8-QTchfGNMCaH4Gk0ozhiAEFP0tXTVNgm99ja6VtXJpu6dSXa-KztV9bVqY6LK6PYuHhLXJn5rq0bFrrJt14fk_GVsVOW2sW28RVcbVQd7d5oztH57XS-W6erz_WPxskpLKnFMuc4VsEwYg3MmBUiqpWEiA5BaUZpZDaAM10xqCXmmM0FZqYmljBswnM3Q4zF2aPrT2xCLxoXS1kNhOxQr-BDEscR4IJ-OZOm7ELzdFDvvGuUPBcHFaLAYDY7HaHDAH07BvW6s-YPPytgvqVlteA</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>755970900</pqid></control><display><type>article</type><title>High subretinal fluid procoagulant activity in rhegmatogenous retinal detachment</title><source>MEDLINE</source><source>Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals</source><source>PubMed Central</source><creator>Ricker, Lukas J A G ; Dieri, Raed Al ; Beckers, Gerold J M ; Pels, Elisabeth ; Liem, Albert T A ; Hendrikse, Fred ; Kijlstra, Aize ; Hemker, H Coenraad ; La Heij, Ellen C</creator><creatorcontrib>Ricker, Lukas J A G ; Dieri, Raed Al ; Beckers, Gerold J M ; Pels, Elisabeth ; Liem, Albert T A ; Hendrikse, Fred ; Kijlstra, Aize ; Hemker, H Coenraad ; La Heij, Ellen C</creatorcontrib><description>An increased mRNA expression of genes related to blood coagulation has been demonstrated in an experimental retinal detachment model but has not yet been confirmed in human clinical specimens. Tissue factor (TF), the initiating factor of blood coagulation, may be a determinant of the extent of tissue injury after rhegmatogenous retinal detachment (RRD). This study was conducted to determine whether subretinal fluid and vitreous fluid collected from patients with RRD have a procoagulant effect.
Calibrated thrombin generation (CAT) was used to investigate the thrombogenic properties of 28 subretinal fluids collected during scleral buckling surgery for RRD. Further, the thrombogenic properties of vitreous fluids from RRD (n = 12), macular pucker (n = 5), macular hole (n = 6), and proliferative diabetic retinopathy (n = 5) were compared with the properties of eye bank eyes (n = 11), which served as control specimens. The procoagulant activity of TF was determined with Western blot analysis.
The addition of subretinal fluid from all RRD patients (28/28, 100%) induced thrombin generation in normal and severely factor (F)XII-deficient plasma. Contrary to the subretinal fluid, the addition of vitreous fluids from various ocular disorders evoked very little thrombin generation in normal and severely FXII-deficient plasma (4/12, 33% RRD; 1/5, 20% macular pucker; 0/6, 0% macular hole; 0/5, 0% proliferative diabetic retinopathy; and 2/11, 18% eye bank eyes). The procoagulant activity in subretinal fluid was almost completely neutralized by antibodies against human TF. The presence of TF in subretinal fluid was confirmed by Western blot.
Subretinal fluid of patients with RRD induces high procoagulant activity, determined by measuring the level of tissue factor.</description><identifier>ISSN: 1552-5783</identifier><identifier>EISSN: 1552-5783</identifier><identifier>DOI: 10.1167/iovs.10-5354</identifier><identifier>PMID: 20435588</identifier><language>eng</language><publisher>United States</publisher><subject>Adolescent ; Adult ; Aged ; Aged, 80 and over ; Blotting, Western ; Body Fluids - metabolism ; Diabetic Retinopathy - metabolism ; Exudates and Transudates - metabolism ; Female ; Humans ; Macular Degeneration - metabolism ; Male ; Middle Aged ; Retinal Detachment - metabolism ; Retinal Detachment - surgery ; Retinal Perforations - metabolism ; Scleral Buckling ; Thrombin - analysis ; Thromboplastin - metabolism ; Vitreous Body - metabolism ; Young Adult</subject><ispartof>Investigative ophthalmology & visual science, 2010-10, Vol.51 (10), p.5234-5239</ispartof><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c290t-7b6a5348dd06398592b9d384559ba224eb55ad7b39b9564b4823cb1e237d5d73</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27901,27902</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20435588$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Ricker, Lukas J A G</creatorcontrib><creatorcontrib>Dieri, Raed Al</creatorcontrib><creatorcontrib>Beckers, Gerold J M</creatorcontrib><creatorcontrib>Pels, Elisabeth</creatorcontrib><creatorcontrib>Liem, Albert T A</creatorcontrib><creatorcontrib>Hendrikse, Fred</creatorcontrib><creatorcontrib>Kijlstra, Aize</creatorcontrib><creatorcontrib>Hemker, H Coenraad</creatorcontrib><creatorcontrib>La Heij, Ellen C</creatorcontrib><title>High subretinal fluid procoagulant activity in rhegmatogenous retinal detachment</title><title>Investigative ophthalmology & visual science</title><addtitle>Invest Ophthalmol Vis Sci</addtitle><description>An increased mRNA expression of genes related to blood coagulation has been demonstrated in an experimental retinal detachment model but has not yet been confirmed in human clinical specimens. Tissue factor (TF), the initiating factor of blood coagulation, may be a determinant of the extent of tissue injury after rhegmatogenous retinal detachment (RRD). This study was conducted to determine whether subretinal fluid and vitreous fluid collected from patients with RRD have a procoagulant effect.
Calibrated thrombin generation (CAT) was used to investigate the thrombogenic properties of 28 subretinal fluids collected during scleral buckling surgery for RRD. Further, the thrombogenic properties of vitreous fluids from RRD (n = 12), macular pucker (n = 5), macular hole (n = 6), and proliferative diabetic retinopathy (n = 5) were compared with the properties of eye bank eyes (n = 11), which served as control specimens. The procoagulant activity of TF was determined with Western blot analysis.
The addition of subretinal fluid from all RRD patients (28/28, 100%) induced thrombin generation in normal and severely factor (F)XII-deficient plasma. Contrary to the subretinal fluid, the addition of vitreous fluids from various ocular disorders evoked very little thrombin generation in normal and severely FXII-deficient plasma (4/12, 33% RRD; 1/5, 20% macular pucker; 0/6, 0% macular hole; 0/5, 0% proliferative diabetic retinopathy; and 2/11, 18% eye bank eyes). The procoagulant activity in subretinal fluid was almost completely neutralized by antibodies against human TF. The presence of TF in subretinal fluid was confirmed by Western blot.
Subretinal fluid of patients with RRD induces high procoagulant activity, determined by measuring the level of tissue factor.</description><subject>Adolescent</subject><subject>Adult</subject><subject>Aged</subject><subject>Aged, 80 and over</subject><subject>Blotting, Western</subject><subject>Body Fluids - metabolism</subject><subject>Diabetic Retinopathy - metabolism</subject><subject>Exudates and Transudates - metabolism</subject><subject>Female</subject><subject>Humans</subject><subject>Macular Degeneration - metabolism</subject><subject>Male</subject><subject>Middle Aged</subject><subject>Retinal Detachment - metabolism</subject><subject>Retinal Detachment - surgery</subject><subject>Retinal Perforations - metabolism</subject><subject>Scleral Buckling</subject><subject>Thrombin - analysis</subject><subject>Thromboplastin - metabolism</subject><subject>Vitreous Body - metabolism</subject><subject>Young Adult</subject><issn>1552-5783</issn><issn>1552-5783</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkE1LAzEQhoMotlZvnmVvXtyaj51NcpSiVijoofcl2aTbyH7UJFvov3eXtuJpZuCZl5cHoXuC54Tk_Nl1-zAnOAUG2QWaEgCaAhfs8t8-QTchfGNMCaH4Gk0ozhiAEFP0tXTVNgm99ja6VtXJpu6dSXa-KztV9bVqY6LK6PYuHhLXJn5rq0bFrrJt14fk_GVsVOW2sW28RVcbVQd7d5oztH57XS-W6erz_WPxskpLKnFMuc4VsEwYg3MmBUiqpWEiA5BaUZpZDaAM10xqCXmmM0FZqYmljBswnM3Q4zF2aPrT2xCLxoXS1kNhOxQr-BDEscR4IJ-OZOm7ELzdFDvvGuUPBcHFaLAYDY7HaHDAH07BvW6s-YPPytgvqVlteA</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Ricker, Lukas J A G</creator><creator>Dieri, Raed Al</creator><creator>Beckers, Gerold J M</creator><creator>Pels, Elisabeth</creator><creator>Liem, Albert T A</creator><creator>Hendrikse, Fred</creator><creator>Kijlstra, Aize</creator><creator>Hemker, H Coenraad</creator><creator>La Heij, Ellen C</creator><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201010</creationdate><title>High subretinal fluid procoagulant activity in rhegmatogenous retinal detachment</title><author>Ricker, Lukas J A G ; Dieri, Raed Al ; Beckers, Gerold J M ; Pels, Elisabeth ; Liem, Albert T A ; Hendrikse, Fred ; Kijlstra, Aize ; Hemker, H Coenraad ; La Heij, Ellen C</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c290t-7b6a5348dd06398592b9d384559ba224eb55ad7b39b9564b4823cb1e237d5d73</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Adolescent</topic><topic>Adult</topic><topic>Aged</topic><topic>Aged, 80 and over</topic><topic>Blotting, Western</topic><topic>Body Fluids - metabolism</topic><topic>Diabetic Retinopathy - metabolism</topic><topic>Exudates and Transudates - metabolism</topic><topic>Female</topic><topic>Humans</topic><topic>Macular Degeneration - metabolism</topic><topic>Male</topic><topic>Middle Aged</topic><topic>Retinal Detachment - metabolism</topic><topic>Retinal Detachment - surgery</topic><topic>Retinal Perforations - metabolism</topic><topic>Scleral Buckling</topic><topic>Thrombin - analysis</topic><topic>Thromboplastin - metabolism</topic><topic>Vitreous Body - metabolism</topic><topic>Young Adult</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Ricker, Lukas J A G</creatorcontrib><creatorcontrib>Dieri, Raed Al</creatorcontrib><creatorcontrib>Beckers, Gerold J M</creatorcontrib><creatorcontrib>Pels, Elisabeth</creatorcontrib><creatorcontrib>Liem, Albert T A</creatorcontrib><creatorcontrib>Hendrikse, Fred</creatorcontrib><creatorcontrib>Kijlstra, Aize</creatorcontrib><creatorcontrib>Hemker, H Coenraad</creatorcontrib><creatorcontrib>La Heij, Ellen C</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Investigative ophthalmology & visual science</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Ricker, Lukas J A G</au><au>Dieri, Raed Al</au><au>Beckers, Gerold J M</au><au>Pels, Elisabeth</au><au>Liem, Albert T A</au><au>Hendrikse, Fred</au><au>Kijlstra, Aize</au><au>Hemker, H Coenraad</au><au>La Heij, Ellen C</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>High subretinal fluid procoagulant activity in rhegmatogenous retinal detachment</atitle><jtitle>Investigative ophthalmology & visual science</jtitle><addtitle>Invest Ophthalmol Vis Sci</addtitle><date>2010-10</date><risdate>2010</risdate><volume>51</volume><issue>10</issue><spage>5234</spage><epage>5239</epage><pages>5234-5239</pages><issn>1552-5783</issn><eissn>1552-5783</eissn><abstract>An increased mRNA expression of genes related to blood coagulation has been demonstrated in an experimental retinal detachment model but has not yet been confirmed in human clinical specimens. Tissue factor (TF), the initiating factor of blood coagulation, may be a determinant of the extent of tissue injury after rhegmatogenous retinal detachment (RRD). This study was conducted to determine whether subretinal fluid and vitreous fluid collected from patients with RRD have a procoagulant effect.
Calibrated thrombin generation (CAT) was used to investigate the thrombogenic properties of 28 subretinal fluids collected during scleral buckling surgery for RRD. Further, the thrombogenic properties of vitreous fluids from RRD (n = 12), macular pucker (n = 5), macular hole (n = 6), and proliferative diabetic retinopathy (n = 5) were compared with the properties of eye bank eyes (n = 11), which served as control specimens. The procoagulant activity of TF was determined with Western blot analysis.
The addition of subretinal fluid from all RRD patients (28/28, 100%) induced thrombin generation in normal and severely factor (F)XII-deficient plasma. Contrary to the subretinal fluid, the addition of vitreous fluids from various ocular disorders evoked very little thrombin generation in normal and severely FXII-deficient plasma (4/12, 33% RRD; 1/5, 20% macular pucker; 0/6, 0% macular hole; 0/5, 0% proliferative diabetic retinopathy; and 2/11, 18% eye bank eyes). The procoagulant activity in subretinal fluid was almost completely neutralized by antibodies against human TF. The presence of TF in subretinal fluid was confirmed by Western blot.
Subretinal fluid of patients with RRD induces high procoagulant activity, determined by measuring the level of tissue factor.</abstract><cop>United States</cop><pmid>20435588</pmid><doi>10.1167/iovs.10-5354</doi><tpages>6</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 1552-5783 |
| ispartof | Investigative ophthalmology & visual science, 2010-10, Vol.51 (10), p.5234-5239 |
| issn | 1552-5783 1552-5783 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_755970900 |
| source | MEDLINE; Elektronische Zeitschriftenbibliothek - Frei zugängliche E-Journals; PubMed Central |
| subjects | Adolescent Adult Aged Aged, 80 and over Blotting, Western Body Fluids - metabolism Diabetic Retinopathy - metabolism Exudates and Transudates - metabolism Female Humans Macular Degeneration - metabolism Male Middle Aged Retinal Detachment - metabolism Retinal Detachment - surgery Retinal Perforations - metabolism Scleral Buckling Thrombin - analysis Thromboplastin - metabolism Vitreous Body - metabolism Young Adult |
| title | High subretinal fluid procoagulant activity in rhegmatogenous retinal detachment |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2025-02-09T09%3A53%3A41IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=High%20subretinal%20fluid%20procoagulant%20activity%20in%20rhegmatogenous%20retinal%20detachment&rft.jtitle=Investigative%20ophthalmology%20&%20visual%20science&rft.au=Ricker,%20Lukas%20J%20A%20G&rft.date=2010-10&rft.volume=51&rft.issue=10&rft.spage=5234&rft.epage=5239&rft.pages=5234-5239&rft.issn=1552-5783&rft.eissn=1552-5783&rft_id=info:doi/10.1167/iovs.10-5354&rft_dat=%3Cproquest_cross%3E755970900%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=755970900&rft_id=info:pmid/20435588&rfr_iscdi=true |