Gentamicin alters nitric oxide production in semicircular canals and otolith organs
Objectives/Hypothesis: Intratympanic application of gentamicin is an important therapeutic option to control vertigo spells in Ménière's disease. Low doses eliminate the function of semicircular canal ampullae (SCCA) and saccule in most patients, although utricular function is maintained in man...
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| Veröffentlicht in: | The Laryngoscope 2010-10, Vol.120 (10), p.2125-2128 |
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| creator | Helling, Kai Heinrich, Ulf-Rüdiger Heusgen, Lukas Brieger, Jürgen Schmidtmann, Irene Li, Huige Mann, Wolf J. |
| description | Objectives/Hypothesis:
Intratympanic application of gentamicin is an important therapeutic option to control vertigo spells in Ménière's disease. Low doses eliminate the function of semicircular canal ampullae (SCCA) and saccule in most patients, although utricular function is maintained in many cases. Local alteration in free radical production might be responsible for these differences. Therefore, the gentamicin‐induced nitric oxide (NO) production was determined in an animal model using separate organ cultures of SCCA, saccule, and utricle.
Study Design:
Prospective pilot study in male guinea pigs.
Methods:
SCCA, saccule, and utricle of 28 guinea pigs were isolated and incubated separately for 6 hours in cell culture medium. Gentamicin was administered in two different concentrations (0.4 mg/mL and 0.8 mg/mL) to organ cultures of 16 animals. Tissues from 12 animals were used as controls. Nitric oxide was quantified by chemiluminescence.
Results:
Gentamicin led to an NO increase of about 70% in the saccule, an NO reduction of more than 70% in SCCA, and an NO reduction of 36% in the utricle.
Conclusions:
The selective effects of gentamicin on the NO production in the different sensory areas of the vestibular organ have to be taken into account in the therapy of Ménière's disease. Laryngoscope, 2010 |
| doi_str_mv | 10.1002/lary.21075 |
| format | Article |
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Intratympanic application of gentamicin is an important therapeutic option to control vertigo spells in Ménière's disease. Low doses eliminate the function of semicircular canal ampullae (SCCA) and saccule in most patients, although utricular function is maintained in many cases. Local alteration in free radical production might be responsible for these differences. Therefore, the gentamicin‐induced nitric oxide (NO) production was determined in an animal model using separate organ cultures of SCCA, saccule, and utricle.
Study Design:
Prospective pilot study in male guinea pigs.
Methods:
SCCA, saccule, and utricle of 28 guinea pigs were isolated and incubated separately for 6 hours in cell culture medium. Gentamicin was administered in two different concentrations (0.4 mg/mL and 0.8 mg/mL) to organ cultures of 16 animals. Tissues from 12 animals were used as controls. Nitric oxide was quantified by chemiluminescence.
Results:
Gentamicin led to an NO increase of about 70% in the saccule, an NO reduction of more than 70% in SCCA, and an NO reduction of 36% in the utricle.
Conclusions:
The selective effects of gentamicin on the NO production in the different sensory areas of the vestibular organ have to be taken into account in the therapy of Ménière's disease. Laryngoscope, 2010</description><identifier>ISSN: 0023-852X</identifier><identifier>EISSN: 1531-4995</identifier><identifier>DOI: 10.1002/lary.21075</identifier><identifier>PMID: 20824779</identifier><identifier>CODEN: LARYA8</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Aminoglycoside ; Animals ; Antibacterial agents ; Antibiotics. Antiinfectious agents. Antiparasitic agents ; Biological and medical sciences ; Gentamicins - pharmacology ; Guinea Pigs ; inner ear ; Level of Evidence: 3a ; Linear Models ; Male ; Medical sciences ; Nitric Oxide - metabolism ; organ culture ; Otolithic Membrane - drug effects ; Otolithic Membrane - secretion ; Otorhinolaryngology. Stomatology ; Pharmacology. Drug treatments ; Pilot Projects ; Prospective Studies ; saccule ; Semicircular Canals - drug effects ; Semicircular Canals - secretion ; utricle</subject><ispartof>The Laryngoscope, 2010-10, Vol.120 (10), p.2125-2128</ispartof><rights>Copyright © 2010 The American Laryngological, Rhinological, and Otological Society, Inc.</rights><rights>2015 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3965-d94b00a25d4bf99eed79127ee6bdff7aed924b89748822ba8647c398c6a209e33</citedby><cites>FETCH-LOGICAL-c3965-d94b00a25d4bf99eed79127ee6bdff7aed924b89748822ba8647c398c6a209e33</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Flary.21075$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Flary.21075$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1416,27922,27923,45572,45573</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23313071$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20824779$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Helling, Kai</creatorcontrib><creatorcontrib>Heinrich, Ulf-Rüdiger</creatorcontrib><creatorcontrib>Heusgen, Lukas</creatorcontrib><creatorcontrib>Brieger, Jürgen</creatorcontrib><creatorcontrib>Schmidtmann, Irene</creatorcontrib><creatorcontrib>Li, Huige</creatorcontrib><creatorcontrib>Mann, Wolf J.</creatorcontrib><title>Gentamicin alters nitric oxide production in semicircular canals and otolith organs</title><title>The Laryngoscope</title><addtitle>The Laryngoscope</addtitle><description>Objectives/Hypothesis:
Intratympanic application of gentamicin is an important therapeutic option to control vertigo spells in Ménière's disease. Low doses eliminate the function of semicircular canal ampullae (SCCA) and saccule in most patients, although utricular function is maintained in many cases. Local alteration in free radical production might be responsible for these differences. Therefore, the gentamicin‐induced nitric oxide (NO) production was determined in an animal model using separate organ cultures of SCCA, saccule, and utricle.
Study Design:
Prospective pilot study in male guinea pigs.
Methods:
SCCA, saccule, and utricle of 28 guinea pigs were isolated and incubated separately for 6 hours in cell culture medium. Gentamicin was administered in two different concentrations (0.4 mg/mL and 0.8 mg/mL) to organ cultures of 16 animals. Tissues from 12 animals were used as controls. Nitric oxide was quantified by chemiluminescence.
Results:
Gentamicin led to an NO increase of about 70% in the saccule, an NO reduction of more than 70% in SCCA, and an NO reduction of 36% in the utricle.
Conclusions:
The selective effects of gentamicin on the NO production in the different sensory areas of the vestibular organ have to be taken into account in the therapy of Ménière's disease. Laryngoscope, 2010</description><subject>Aminoglycoside</subject><subject>Animals</subject><subject>Antibacterial agents</subject><subject>Antibiotics. Antiinfectious agents. Antiparasitic agents</subject><subject>Biological and medical sciences</subject><subject>Gentamicins - pharmacology</subject><subject>Guinea Pigs</subject><subject>inner ear</subject><subject>Level of Evidence: 3a</subject><subject>Linear Models</subject><subject>Male</subject><subject>Medical sciences</subject><subject>Nitric Oxide - metabolism</subject><subject>organ culture</subject><subject>Otolithic Membrane - drug effects</subject><subject>Otolithic Membrane - secretion</subject><subject>Otorhinolaryngology. Stomatology</subject><subject>Pharmacology. Drug treatments</subject><subject>Pilot Projects</subject><subject>Prospective Studies</subject><subject>saccule</subject><subject>Semicircular Canals - drug effects</subject><subject>Semicircular Canals - secretion</subject><subject>utricle</subject><issn>0023-852X</issn><issn>1531-4995</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp90MtO3DAUBmCrooIpZcMDIG8QUqVQX-I4XnIpQ6tRK5VWhZXl2CdgyDhTOxHw9niYAXasvDjff471I7RLySElhH3tTHw8ZJRI8QFNqOC0KJUSG2iSh7yoBbvcQp9SuiWESi7IJtpipGallGqCLqYQBjP31gdsugFiwsEP0VvcP3gHeBF7N9rB9wFnkWApox3zSWxNMF3CJjjcD33nhxvcx2sT0mf0sc0T2Fm_2-jv2bc_J-fF7Nf0-8nRrLBcVaJwqmwIMUy4smmVAnBSUSYBqsa1rTTgFCubWsmyrhlrTF2VMidrWxlGFHC-jQ5We_Mn_4-QBj33yULXmQD9mLQUQlWKkSrLLytpY59ShFYvop_n2jQletmhXnaonzvMeG-9dmzm4F7pS2kZ7K-BSdZ0bTTB-vTmOKecSJodXbl738HjOyf17Oj31cvxYpXxaYCH14yJd7qSPNN_P6f69PSY_liuqPgThjyZDw</recordid><startdate>201010</startdate><enddate>201010</enddate><creator>Helling, Kai</creator><creator>Heinrich, Ulf-Rüdiger</creator><creator>Heusgen, Lukas</creator><creator>Brieger, Jürgen</creator><creator>Schmidtmann, Irene</creator><creator>Li, Huige</creator><creator>Mann, Wolf J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Blackwell</general><scope>BSCLL</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>201010</creationdate><title>Gentamicin alters nitric oxide production in semicircular canals and otolith organs</title><author>Helling, Kai ; Heinrich, Ulf-Rüdiger ; Heusgen, Lukas ; Brieger, Jürgen ; Schmidtmann, Irene ; Li, Huige ; Mann, Wolf J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3965-d94b00a25d4bf99eed79127ee6bdff7aed924b89748822ba8647c398c6a209e33</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aminoglycoside</topic><topic>Animals</topic><topic>Antibacterial agents</topic><topic>Antibiotics. Antiinfectious agents. Antiparasitic agents</topic><topic>Biological and medical sciences</topic><topic>Gentamicins - pharmacology</topic><topic>Guinea Pigs</topic><topic>inner ear</topic><topic>Level of Evidence: 3a</topic><topic>Linear Models</topic><topic>Male</topic><topic>Medical sciences</topic><topic>Nitric Oxide - metabolism</topic><topic>organ culture</topic><topic>Otolithic Membrane - drug effects</topic><topic>Otolithic Membrane - secretion</topic><topic>Otorhinolaryngology. Stomatology</topic><topic>Pharmacology. Drug treatments</topic><topic>Pilot Projects</topic><topic>Prospective Studies</topic><topic>saccule</topic><topic>Semicircular Canals - drug effects</topic><topic>Semicircular Canals - secretion</topic><topic>utricle</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Helling, Kai</creatorcontrib><creatorcontrib>Heinrich, Ulf-Rüdiger</creatorcontrib><creatorcontrib>Heusgen, Lukas</creatorcontrib><creatorcontrib>Brieger, Jürgen</creatorcontrib><creatorcontrib>Schmidtmann, Irene</creatorcontrib><creatorcontrib>Li, Huige</creatorcontrib><creatorcontrib>Mann, Wolf J.</creatorcontrib><collection>Istex</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Laryngoscope</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Helling, Kai</au><au>Heinrich, Ulf-Rüdiger</au><au>Heusgen, Lukas</au><au>Brieger, Jürgen</au><au>Schmidtmann, Irene</au><au>Li, Huige</au><au>Mann, Wolf J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Gentamicin alters nitric oxide production in semicircular canals and otolith organs</atitle><jtitle>The Laryngoscope</jtitle><addtitle>The Laryngoscope</addtitle><date>2010-10</date><risdate>2010</risdate><volume>120</volume><issue>10</issue><spage>2125</spage><epage>2128</epage><pages>2125-2128</pages><issn>0023-852X</issn><eissn>1531-4995</eissn><coden>LARYA8</coden><abstract>Objectives/Hypothesis:
Intratympanic application of gentamicin is an important therapeutic option to control vertigo spells in Ménière's disease. Low doses eliminate the function of semicircular canal ampullae (SCCA) and saccule in most patients, although utricular function is maintained in many cases. Local alteration in free radical production might be responsible for these differences. Therefore, the gentamicin‐induced nitric oxide (NO) production was determined in an animal model using separate organ cultures of SCCA, saccule, and utricle.
Study Design:
Prospective pilot study in male guinea pigs.
Methods:
SCCA, saccule, and utricle of 28 guinea pigs were isolated and incubated separately for 6 hours in cell culture medium. Gentamicin was administered in two different concentrations (0.4 mg/mL and 0.8 mg/mL) to organ cultures of 16 animals. Tissues from 12 animals were used as controls. Nitric oxide was quantified by chemiluminescence.
Results:
Gentamicin led to an NO increase of about 70% in the saccule, an NO reduction of more than 70% in SCCA, and an NO reduction of 36% in the utricle.
Conclusions:
The selective effects of gentamicin on the NO production in the different sensory areas of the vestibular organ have to be taken into account in the therapy of Ménière's disease. Laryngoscope, 2010</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>20824779</pmid><doi>10.1002/lary.21075</doi><tpages>4</tpages></addata></record> |
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| source | MEDLINE; Wiley Online Library Journals Frontfile Complete |
| subjects | Aminoglycoside Animals Antibacterial agents Antibiotics. Antiinfectious agents. Antiparasitic agents Biological and medical sciences Gentamicins - pharmacology Guinea Pigs inner ear Level of Evidence: 3a Linear Models Male Medical sciences Nitric Oxide - metabolism organ culture Otolithic Membrane - drug effects Otolithic Membrane - secretion Otorhinolaryngology. Stomatology Pharmacology. Drug treatments Pilot Projects Prospective Studies saccule Semicircular Canals - drug effects Semicircular Canals - secretion utricle |
| title | Gentamicin alters nitric oxide production in semicircular canals and otolith organs |
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