The role of subcellular factors in pulmonary immune function: physicochemical characterization of two distinct species of lymphocyte- activating factor produced by rabbit alveolar macrophages
T cell stimulatory factors produced by rabbit alveolar macrophages were investigated. Physicochemical characterization revealed that alveolar macrophages (harvested by bronchopulmonary lavage and stimulated in tissue culture with bacterial lipopolysaccharide) released 2 predominant species of lympho...
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| Veröffentlicht in: | The Journal of immunology (1950) 1981-04, Vol.126 (4), p.1534-1541 |
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| container_title | The Journal of immunology (1950) |
| container_volume | 126 |
| creator | Simon, PL Willoughby, WF |
| description | T cell stimulatory factors produced by rabbit alveolar macrophages were investigated. Physicochemical characterization revealed that alveolar macrophages (harvested by bronchopulmonary lavage and stimulated in tissue culture with bacterial lipopolysaccharide) released 2 predominant species of lymphocyte-activating factor (LAF) with isoelectric points of 4.6 and 7.4, and m.w. of 14,400 and 11,600 daltons, respectively, as calculated by the Svedberg equation. Using C3H/HeJ mouse thymocytes (and in some instances nylon wool-purified nonadherent rabbit spleen or lymph node cells) as target cells, rabbit LAF was found to induce proliferative responses directly, as well as enhance proliferative responses to phytomitogens. Both LAF species were inactivated by heating, treatment with trypsin, or at low (2.3) pH. The pI 7.4 LAF was also unstable at high pH (9.0). The thymocyte stimulatory activity of both LAF species was not inhibited by the anti-proteases alpha-1-anti-trypsin, Traysylol (aprotinin), leupeptin, or pepstatin. |
| doi_str_mv | 10.4049/jimmunol.126.4.1534 |
| format | Article |
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Physicochemical characterization revealed that alveolar macrophages (harvested by bronchopulmonary lavage and stimulated in tissue culture with bacterial lipopolysaccharide) released 2 predominant species of lymphocyte-activating factor (LAF) with isoelectric points of 4.6 and 7.4, and m.w. of 14,400 and 11,600 daltons, respectively, as calculated by the Svedberg equation. Using C3H/HeJ mouse thymocytes (and in some instances nylon wool-purified nonadherent rabbit spleen or lymph node cells) as target cells, rabbit LAF was found to induce proliferative responses directly, as well as enhance proliferative responses to phytomitogens. Both LAF species were inactivated by heating, treatment with trypsin, or at low (2.3) pH. The pI 7.4 LAF was also unstable at high pH (9.0). The thymocyte stimulatory activity of both LAF species was not inhibited by the anti-proteases alpha-1-anti-trypsin, Traysylol (aprotinin), leupeptin, or pepstatin.</description><subject>Animals</subject><subject>Cells, Cultured</subject><subject>Centrifugation, Density Gradient</subject><subject>Chemical Phenomena</subject><subject>Chemistry, Physical</subject><subject>Female</subject><subject>Hot Temperature</subject><subject>Hydrogen-Ion Concentration</subject><subject>Interleukin-1</subject><subject>Lipopolysaccharides - pharmacology</subject><subject>Macrophages - immunology</subject><subject>Male</subject><subject>Mice</subject><subject>Mice, Inbred C3H</subject><subject>Mycobacterium bovis - immunology</subject><subject>Protease Inhibitors - pharmacology</subject><subject>Proteins - immunology</subject><subject>Proteins - pharmacology</subject><subject>Pulmonary Alveoli - immunology</subject><subject>Rabbits</subject><subject>Subcellular Fractions - immunology</subject><subject>Trypsin - pharmacology</subject><issn>0022-1767</issn><issn>1550-6606</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1981</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNpNkc9u1DAQxi1EVZaFJ0BIPsEpW9txnIYbqsofqVIv7dmyncnGlR0HO2kUXo5Xw2EXhC8jeb7fN6P5EHpHyYET3lw9We_nIbgDZeLAD7Qq-Qu0o1VFCiGIeIl2hDBW0FrUr9DrlJ4IIYIwfokuRX3NqGA79OuhBxyDAxw6nGZtwLnZqYg7ZaYQE7YDHmfnw6Diiv8MBNzNg5lsGD7hsV-TNcH04K1RDptexQxCtD_VpthcpyXg1qbJZginEYyFtP271Y99MOsEBc6Mfc7EcDwPxmMM7WygxXrFUWltJ6zcM4RtN69MDGOvjpDeoItOuQRvz3WPHr_cPtx8K-7uv36_-XxXGNYwXlQ1N1ToxjSEC12bjpCOARhd80YBZ6ZltGWiuuaC8a5jpTGspFrrkinNKSn36MPJN-_1Y4Y0SW_Tdiw1QJiTrKuqqcr89qg8CfOKKUXo5Bitz8eTlMgtNvk3Npljk1xusWXq_dl-1h7af8w5p9z_eOr39tgvNoJMXjmX1VQuy_Kf028C5ang</recordid><startdate>198104</startdate><enddate>198104</enddate><creator>Simon, PL</creator><creator>Willoughby, WF</creator><general>Am Assoc Immnol</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198104</creationdate><title>The role of subcellular factors in pulmonary immune function: physicochemical characterization of two distinct species of lymphocyte- activating factor produced by rabbit alveolar macrophages</title><author>Simon, PL ; Willoughby, WF</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2924-574c16b9c9046b7cf00f2eecb749ae42cd21d26584624ff23cc231bbb32ab4103</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1981</creationdate><topic>Animals</topic><topic>Cells, Cultured</topic><topic>Centrifugation, Density Gradient</topic><topic>Chemical Phenomena</topic><topic>Chemistry, Physical</topic><topic>Female</topic><topic>Hot Temperature</topic><topic>Hydrogen-Ion Concentration</topic><topic>Interleukin-1</topic><topic>Lipopolysaccharides - pharmacology</topic><topic>Macrophages - immunology</topic><topic>Male</topic><topic>Mice</topic><topic>Mice, Inbred C3H</topic><topic>Mycobacterium bovis - immunology</topic><topic>Protease Inhibitors - pharmacology</topic><topic>Proteins - immunology</topic><topic>Proteins - pharmacology</topic><topic>Pulmonary Alveoli - immunology</topic><topic>Rabbits</topic><topic>Subcellular Fractions - immunology</topic><topic>Trypsin - pharmacology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Simon, PL</creatorcontrib><creatorcontrib>Willoughby, WF</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of immunology (1950)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Simon, PL</au><au>Willoughby, WF</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>The role of subcellular factors in pulmonary immune function: physicochemical characterization of two distinct species of lymphocyte- activating factor produced by rabbit alveolar macrophages</atitle><jtitle>The Journal of immunology (1950)</jtitle><addtitle>J Immunol</addtitle><date>1981-04</date><risdate>1981</risdate><volume>126</volume><issue>4</issue><spage>1534</spage><epage>1541</epage><pages>1534-1541</pages><issn>0022-1767</issn><eissn>1550-6606</eissn><abstract>T cell stimulatory factors produced by rabbit alveolar macrophages were investigated. Physicochemical characterization revealed that alveolar macrophages (harvested by bronchopulmonary lavage and stimulated in tissue culture with bacterial lipopolysaccharide) released 2 predominant species of lymphocyte-activating factor (LAF) with isoelectric points of 4.6 and 7.4, and m.w. of 14,400 and 11,600 daltons, respectively, as calculated by the Svedberg equation. Using C3H/HeJ mouse thymocytes (and in some instances nylon wool-purified nonadherent rabbit spleen or lymph node cells) as target cells, rabbit LAF was found to induce proliferative responses directly, as well as enhance proliferative responses to phytomitogens. Both LAF species were inactivated by heating, treatment with trypsin, or at low (2.3) pH. The pI 7.4 LAF was also unstable at high pH (9.0). The thymocyte stimulatory activity of both LAF species was not inhibited by the anti-proteases alpha-1-anti-trypsin, Traysylol (aprotinin), leupeptin, or pepstatin.</abstract><cop>United States</cop><pub>Am Assoc Immnol</pub><pmid>6782162</pmid><doi>10.4049/jimmunol.126.4.1534</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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| identifier | ISSN: 0022-1767 |
| ispartof | The Journal of immunology (1950), 1981-04, Vol.126 (4), p.1534-1541 |
| issn | 0022-1767 1550-6606 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75595333 |
| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Cells, Cultured Centrifugation, Density Gradient Chemical Phenomena Chemistry, Physical Female Hot Temperature Hydrogen-Ion Concentration Interleukin-1 Lipopolysaccharides - pharmacology Macrophages - immunology Male Mice Mice, Inbred C3H Mycobacterium bovis - immunology Protease Inhibitors - pharmacology Proteins - immunology Proteins - pharmacology Pulmonary Alveoli - immunology Rabbits Subcellular Fractions - immunology Trypsin - pharmacology |
| title | The role of subcellular factors in pulmonary immune function: physicochemical characterization of two distinct species of lymphocyte- activating factor produced by rabbit alveolar macrophages |
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