Identification of Tryptophan 2,3‐Dioxygenase RNA in Rodent Brain
: The gene for tryptophan 2,3‐dioxygenase (TDO) heretofore was believed to be expressed only in liver. The data presented here demonstrate that RNA encoding TDO is present in rodent brain. Oligonucleotide primers based on the rat liver TDO cDNA sequence were synthesized and used to amplify RNA deriv...
Gespeichert in:
| Veröffentlicht in: | Journal of neurochemistry 1993-03, Vol.60 (3), p.1159-1162 |
|---|---|
| Hauptverfasser: | , , , , |
| Format: | Artikel |
| Sprache: | eng |
| Schlagworte: | |
| Online-Zugang: | Volltext |
| Tags: |
Tag hinzufügen
Keine Tags, Fügen Sie den ersten Tag hinzu!
|
| container_end_page | 1162 |
|---|---|
| container_issue | 3 |
| container_start_page | 1159 |
| container_title | Journal of neurochemistry |
| container_volume | 60 |
| creator | Haber, Roberta Bessette, Daniel Hulihan‐Giblin, Bridget Durcan, Michael J. Goldman, David |
| description | : The gene for tryptophan 2,3‐dioxygenase (TDO) heretofore was believed to be expressed only in liver. The data presented here demonstrate that RNA encoding TDO is present in rodent brain. Oligonucleotide primers based on the rat liver TDO cDNA sequence were synthesized and used to amplify RNA derived from mouse whole brain and liver and rat brain regions by the RNA‐PCR. Reaction products were purified and subjected to DNA sequencing. Identical sequences were obtained when mouse whole brain and liver RNAs were amplified, and these sequences were shown to be 96% identical to the published rat liver tryptophan TDO cDNA sequence. In addition, TDO sequences were found in RNA derived from rat brainstem, cerebellum, cortex, hypothalamus, and the remainder of the brain. |
| doi_str_mv | 10.1111/j.1471-4159.1993.tb03269.x |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_75595024</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>75595024</sourcerecordid><originalsourceid>FETCH-LOGICAL-c4969-be5f26c3fa5d4a5ba17be1178d0406413b944bac2d17c0da84bd8935be0981073</originalsourceid><addsrcrecordid>eNqVkN1KwzAYhoMoc04vQSgiHtma32bxRLb5z5gw5nFI0lQzunY2HW5nXoLX6JXYsrJTMScf5H3eL-EB4AzBCNXnah4hylFIERMREoJElYYExyJa74HuLtoHXQgxDgmk-BAceT-HEMU0Rh3Q4TEXHJMuGD4lNq9c6oyqXJEHRRrMys2yKpbvKg_wJfn5-r51xXrzZnPlbTCdDAKXB9OiqQXDUrn8GBykKvP2pJ098Hp_Nxs9huOXh6fRYBwaKmIRastSHBuSKpZQxbRCXFuEeD-BFMYUES0o1crgBHEDE9WnOukLwrSFoo8gJz1wsd27LIuPlfWVXDhvbJap3BYrLzljgkFM_wRrCQxz1oDXW9CUhfelTeWydAtVbiSCsjEt57LRKRudsjEtW9NyXZdP21dWemGTXbVVW-fnba68UVlaqtw4v8NojAXBrMZuttiny-zmHx-Qz5MRqq_JLyVYmic</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>16452754</pqid></control><display><type>article</type><title>Identification of Tryptophan 2,3‐Dioxygenase RNA in Rodent Brain</title><source>MEDLINE</source><source>Access via Wiley Online Library</source><creator>Haber, Roberta ; Bessette, Daniel ; Hulihan‐Giblin, Bridget ; Durcan, Michael J. ; Goldman, David</creator><creatorcontrib>Haber, Roberta ; Bessette, Daniel ; Hulihan‐Giblin, Bridget ; Durcan, Michael J. ; Goldman, David</creatorcontrib><description>: The gene for tryptophan 2,3‐dioxygenase (TDO) heretofore was believed to be expressed only in liver. The data presented here demonstrate that RNA encoding TDO is present in rodent brain. Oligonucleotide primers based on the rat liver TDO cDNA sequence were synthesized and used to amplify RNA derived from mouse whole brain and liver and rat brain regions by the RNA‐PCR. Reaction products were purified and subjected to DNA sequencing. Identical sequences were obtained when mouse whole brain and liver RNAs were amplified, and these sequences were shown to be 96% identical to the published rat liver tryptophan TDO cDNA sequence. In addition, TDO sequences were found in RNA derived from rat brainstem, cerebellum, cortex, hypothalamus, and the remainder of the brain.</description><identifier>ISSN: 0022-3042</identifier><identifier>EISSN: 1471-4159</identifier><identifier>DOI: 10.1111/j.1471-4159.1993.tb03269.x</identifier><identifier>PMID: 7679723</identifier><identifier>CODEN: JONRA9</identifier><language>eng</language><publisher>Oxford, UK: Blackwell Publishing Ltd</publisher><subject>Animals ; Base Sequence ; Biochemistry and metabolism ; Biological and medical sciences ; Central nervous system ; DNA - genetics ; Fundamental and applied biological sciences. Psychology ; Mice ; Mice, Inbred Strains ; Molecular Sequence Data ; Oligonucleotide Probes - genetics ; Polymerase Chain Reaction ; Rats ; Rats, Sprague-Dawley ; RNA - genetics ; RNA - metabolism ; RNA amplification ; Rodent brain ; Rodentia ; Tryptophan 2,3‐dioxygenase ; Tryptophan Oxygenase - genetics ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neurochemistry, 1993-03, Vol.60 (3), p.1159-1162</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4969-be5f26c3fa5d4a5ba17be1178d0406413b944bac2d17c0da84bd8935be0981073</citedby><cites>FETCH-LOGICAL-c4969-be5f26c3fa5d4a5ba17be1178d0406413b944bac2d17c0da84bd8935be0981073</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1111%2Fj.1471-4159.1993.tb03269.x$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1111%2Fj.1471-4159.1993.tb03269.x$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4629325$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/7679723$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Haber, Roberta</creatorcontrib><creatorcontrib>Bessette, Daniel</creatorcontrib><creatorcontrib>Hulihan‐Giblin, Bridget</creatorcontrib><creatorcontrib>Durcan, Michael J.</creatorcontrib><creatorcontrib>Goldman, David</creatorcontrib><title>Identification of Tryptophan 2,3‐Dioxygenase RNA in Rodent Brain</title><title>Journal of neurochemistry</title><addtitle>J Neurochem</addtitle><description>: The gene for tryptophan 2,3‐dioxygenase (TDO) heretofore was believed to be expressed only in liver. The data presented here demonstrate that RNA encoding TDO is present in rodent brain. Oligonucleotide primers based on the rat liver TDO cDNA sequence were synthesized and used to amplify RNA derived from mouse whole brain and liver and rat brain regions by the RNA‐PCR. Reaction products were purified and subjected to DNA sequencing. Identical sequences were obtained when mouse whole brain and liver RNAs were amplified, and these sequences were shown to be 96% identical to the published rat liver tryptophan TDO cDNA sequence. In addition, TDO sequences were found in RNA derived from rat brainstem, cerebellum, cortex, hypothalamus, and the remainder of the brain.</description><subject>Animals</subject><subject>Base Sequence</subject><subject>Biochemistry and metabolism</subject><subject>Biological and medical sciences</subject><subject>Central nervous system</subject><subject>DNA - genetics</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Mice</subject><subject>Mice, Inbred Strains</subject><subject>Molecular Sequence Data</subject><subject>Oligonucleotide Probes - genetics</subject><subject>Polymerase Chain Reaction</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>RNA - genetics</subject><subject>RNA - metabolism</subject><subject>RNA amplification</subject><subject>Rodent brain</subject><subject>Rodentia</subject><subject>Tryptophan 2,3‐dioxygenase</subject><subject>Tryptophan Oxygenase - genetics</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3042</issn><issn>1471-4159</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqVkN1KwzAYhoMoc04vQSgiHtma32bxRLb5z5gw5nFI0lQzunY2HW5nXoLX6JXYsrJTMScf5H3eL-EB4AzBCNXnah4hylFIERMREoJElYYExyJa74HuLtoHXQgxDgmk-BAceT-HEMU0Rh3Q4TEXHJMuGD4lNq9c6oyqXJEHRRrMys2yKpbvKg_wJfn5-r51xXrzZnPlbTCdDAKXB9OiqQXDUrn8GBykKvP2pJ098Hp_Nxs9huOXh6fRYBwaKmIRastSHBuSKpZQxbRCXFuEeD-BFMYUES0o1crgBHEDE9WnOukLwrSFoo8gJz1wsd27LIuPlfWVXDhvbJap3BYrLzljgkFM_wRrCQxz1oDXW9CUhfelTeWydAtVbiSCsjEt57LRKRudsjEtW9NyXZdP21dWemGTXbVVW-fnba68UVlaqtw4v8NojAXBrMZuttiny-zmHx-Qz5MRqq_JLyVYmic</recordid><startdate>199303</startdate><enddate>199303</enddate><creator>Haber, Roberta</creator><creator>Bessette, Daniel</creator><creator>Hulihan‐Giblin, Bridget</creator><creator>Durcan, Michael J.</creator><creator>Goldman, David</creator><general>Blackwell Publishing Ltd</general><general>Blackwell</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199303</creationdate><title>Identification of Tryptophan 2,3‐Dioxygenase RNA in Rodent Brain</title><author>Haber, Roberta ; Bessette, Daniel ; Hulihan‐Giblin, Bridget ; Durcan, Michael J. ; Goldman, David</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4969-be5f26c3fa5d4a5ba17be1178d0406413b944bac2d17c0da84bd8935be0981073</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Base Sequence</topic><topic>Biochemistry and metabolism</topic><topic>Biological and medical sciences</topic><topic>Central nervous system</topic><topic>DNA - genetics</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Mice</topic><topic>Mice, Inbred Strains</topic><topic>Molecular Sequence Data</topic><topic>Oligonucleotide Probes - genetics</topic><topic>Polymerase Chain Reaction</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>RNA - genetics</topic><topic>RNA - metabolism</topic><topic>RNA amplification</topic><topic>Rodent brain</topic><topic>Rodentia</topic><topic>Tryptophan 2,3‐dioxygenase</topic><topic>Tryptophan Oxygenase - genetics</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Haber, Roberta</creatorcontrib><creatorcontrib>Bessette, Daniel</creatorcontrib><creatorcontrib>Hulihan‐Giblin, Bridget</creatorcontrib><creatorcontrib>Durcan, Michael J.</creatorcontrib><creatorcontrib>Goldman, David</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Haber, Roberta</au><au>Bessette, Daniel</au><au>Hulihan‐Giblin, Bridget</au><au>Durcan, Michael J.</au><au>Goldman, David</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Identification of Tryptophan 2,3‐Dioxygenase RNA in Rodent Brain</atitle><jtitle>Journal of neurochemistry</jtitle><addtitle>J Neurochem</addtitle><date>1993-03</date><risdate>1993</risdate><volume>60</volume><issue>3</issue><spage>1159</spage><epage>1162</epage><pages>1159-1162</pages><issn>0022-3042</issn><eissn>1471-4159</eissn><coden>JONRA9</coden><abstract>: The gene for tryptophan 2,3‐dioxygenase (TDO) heretofore was believed to be expressed only in liver. The data presented here demonstrate that RNA encoding TDO is present in rodent brain. Oligonucleotide primers based on the rat liver TDO cDNA sequence were synthesized and used to amplify RNA derived from mouse whole brain and liver and rat brain regions by the RNA‐PCR. Reaction products were purified and subjected to DNA sequencing. Identical sequences were obtained when mouse whole brain and liver RNAs were amplified, and these sequences were shown to be 96% identical to the published rat liver tryptophan TDO cDNA sequence. In addition, TDO sequences were found in RNA derived from rat brainstem, cerebellum, cortex, hypothalamus, and the remainder of the brain.</abstract><cop>Oxford, UK</cop><pub>Blackwell Publishing Ltd</pub><pmid>7679723</pmid><doi>10.1111/j.1471-4159.1993.tb03269.x</doi><tpages>4</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0022-3042 |
| ispartof | Journal of neurochemistry, 1993-03, Vol.60 (3), p.1159-1162 |
| issn | 0022-3042 1471-4159 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75595024 |
| source | MEDLINE; Access via Wiley Online Library |
| subjects | Animals Base Sequence Biochemistry and metabolism Biological and medical sciences Central nervous system DNA - genetics Fundamental and applied biological sciences. Psychology Mice Mice, Inbred Strains Molecular Sequence Data Oligonucleotide Probes - genetics Polymerase Chain Reaction Rats Rats, Sprague-Dawley RNA - genetics RNA - metabolism RNA amplification Rodent brain Rodentia Tryptophan 2,3‐dioxygenase Tryptophan Oxygenase - genetics Vertebrates: nervous system and sense organs |
| title | Identification of Tryptophan 2,3‐Dioxygenase RNA in Rodent Brain |
| url | https://sfx.bib-bvb.de/sfx_tum?ctx_ver=Z39.88-2004&ctx_enc=info:ofi/enc:UTF-8&ctx_tim=2024-12-19T08%3A37%3A52IST&url_ver=Z39.88-2004&url_ctx_fmt=infofi/fmt:kev:mtx:ctx&rfr_id=info:sid/primo.exlibrisgroup.com:primo3-Article-proquest_cross&rft_val_fmt=info:ofi/fmt:kev:mtx:journal&rft.genre=article&rft.atitle=Identification%20of%20Tryptophan%202,3%E2%80%90Dioxygenase%20RNA%20in%20Rodent%20Brain&rft.jtitle=Journal%20of%20neurochemistry&rft.au=Haber,%20Roberta&rft.date=1993-03&rft.volume=60&rft.issue=3&rft.spage=1159&rft.epage=1162&rft.pages=1159-1162&rft.issn=0022-3042&rft.eissn=1471-4159&rft.coden=JONRA9&rft_id=info:doi/10.1111/j.1471-4159.1993.tb03269.x&rft_dat=%3Cproquest_cross%3E75595024%3C/proquest_cross%3E%3Curl%3E%3C/url%3E&disable_directlink=true&sfx.directlink=off&sfx.report_link=0&rft_id=info:oai/&rft_pqid=16452754&rft_id=info:pmid/7679723&rfr_iscdi=true |