Sequence and chromosomal localization of two PET genes required for cytochrome c oxidase assembly in Saccharomyces cerevisiae
The nuclear genes PET117 and PET191 are required for the assembly of active cytochrome c oxidase in Saccharomyces cerevisiae, yet their gene products are not subunits of the final assembled cytochrome c oxidase complex. Plasmids bearing PET117 or PET191 were isolated by their ability to complement t...
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| Veröffentlicht in: | Current genetics 1993, Vol.23 (1), p.9-14 |
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| creator | MCEWEN, J. E HERAN HONG, K PARK, S PRECIADO, G. T |
| description | The nuclear genes PET117 and PET191 are required for the assembly of active cytochrome c oxidase in Saccharomyces cerevisiae, yet their gene products are not subunits of the final assembled cytochrome c oxidase complex. Plasmids bearing PET117 or PET191 were isolated by their ability to complement the pet117-1 or pet191-1 mutations, respectively. By restriction mapping, subcloning, and deletion analysis of yeast DNA fragments that complement these mutations, the PET117 and PET191 genes were localized to smaller regions of DNA, which were then sequenced from both strands. The PET117 open reading frame is of 107 codons and the PET191 open reading frame is of 108 codons. Neither the PET191 nor PET117 DNA sequences have been reported previously, and the derived amino-acid sequences of the PET191 and PET117 open reading frames exhibit no significant primary amino-acid sequence similarity to other protein sequences available in the NBRF data base, or from translated Genbank sequences. By hybridization of PET117 or PET191 probes first to a chromosome blot and next to a library of physically mapped fragments of yeast genomic DNA, the map locations of the PET191 and PET117 genes were determined. PET117 is located on chromosome 5 near the HIS1 gene and PET191 is located on chromosome 10 near the CYC1 gene. |
| doi_str_mv | 10.1007/BF00336742 |
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E ; HERAN HONG, K ; PARK, S ; PRECIADO, G. T</creator><creatorcontrib>MCEWEN, J. E ; HERAN HONG, K ; PARK, S ; PRECIADO, G. T ; California Univ., Los Angeles (USA). Dept. of Microbiology. Molecular Biology Inst</creatorcontrib><description>The nuclear genes PET117 and PET191 are required for the assembly of active cytochrome c oxidase in Saccharomyces cerevisiae, yet their gene products are not subunits of the final assembled cytochrome c oxidase complex. Plasmids bearing PET117 or PET191 were isolated by their ability to complement the pet117-1 or pet191-1 mutations, respectively. By restriction mapping, subcloning, and deletion analysis of yeast DNA fragments that complement these mutations, the PET117 and PET191 genes were localized to smaller regions of DNA, which were then sequenced from both strands. The PET117 open reading frame is of 107 codons and the PET191 open reading frame is of 108 codons. Neither the PET191 nor PET117 DNA sequences have been reported previously, and the derived amino-acid sequences of the PET191 and PET117 open reading frames exhibit no significant primary amino-acid sequence similarity to other protein sequences available in the NBRF data base, or from translated Genbank sequences. By hybridization of PET117 or PET191 probes first to a chromosome blot and next to a library of physically mapped fragments of yeast genomic DNA, the map locations of the PET191 and PET117 genes were determined. PET117 is located on chromosome 5 near the HIS1 gene and PET191 is located on chromosome 10 near the CYC1 gene.</description><identifier>ISSN: 0172-8083</identifier><identifier>EISSN: 1432-0983</identifier><identifier>DOI: 10.1007/BF00336742</identifier><identifier>PMID: 8381337</identifier><identifier>CODEN: CUGED5</identifier><language>eng</language><publisher>Heidelberg: Springer</publisher><subject>adn ; Amino Acid Sequence ; Base Sequence ; Biological and medical sciences ; Chromosomes, Fungal ; citocromo c oxidasa ; Cloning, Molecular ; cytochrome c oxidase ; cytochrome c oxydase ; dna ; DNA, Fungal ; Electron Transport Complex IV - genetics ; Electron Transport Complex IV - metabolism ; Fundamental and applied biological sciences. Psychology ; gene ; gene location ; genes ; Genes, Fungal ; Genes. Genome ; Genetic Complementation Test ; localisation de gene ; localizacion de genes ; Membrane Proteins - genetics ; Membrane Proteins - metabolism ; mitochondria ; mitochondrie ; mitocondria ; Molecular and cellular biology ; Molecular genetics ; Molecular Sequence Data ; nucleotide sequence ; Saccharomyces cerevisiae ; Saccharomyces cerevisiae - genetics ; Saccharomyces cerevisiae Proteins - genetics ; Saccharomyces cerevisiae Proteins - metabolism ; secuencia nucleica ; sequence nucleique</subject><ispartof>Current genetics, 1993, Vol.23 (1), p.9-14</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c280t-9b233114f6324842b168d4d41f55f89563ae1ab808c95d8a6eebce173c999f663</citedby><cites>FETCH-LOGICAL-c280t-9b233114f6324842b168d4d41f55f89563ae1ab808c95d8a6eebce173c999f663</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4522501$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8381337$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MCEWEN, J. E</creatorcontrib><creatorcontrib>HERAN HONG, K</creatorcontrib><creatorcontrib>PARK, S</creatorcontrib><creatorcontrib>PRECIADO, G. T</creatorcontrib><creatorcontrib>California Univ., Los Angeles (USA). Dept. of Microbiology. Molecular Biology Inst</creatorcontrib><title>Sequence and chromosomal localization of two PET genes required for cytochrome c oxidase assembly in Saccharomyces cerevisiae</title><title>Current genetics</title><addtitle>Curr Genet</addtitle><description>The nuclear genes PET117 and PET191 are required for the assembly of active cytochrome c oxidase in Saccharomyces cerevisiae, yet their gene products are not subunits of the final assembled cytochrome c oxidase complex. Plasmids bearing PET117 or PET191 were isolated by their ability to complement the pet117-1 or pet191-1 mutations, respectively. By restriction mapping, subcloning, and deletion analysis of yeast DNA fragments that complement these mutations, the PET117 and PET191 genes were localized to smaller regions of DNA, which were then sequenced from both strands. The PET117 open reading frame is of 107 codons and the PET191 open reading frame is of 108 codons. Neither the PET191 nor PET117 DNA sequences have been reported previously, and the derived amino-acid sequences of the PET191 and PET117 open reading frames exhibit no significant primary amino-acid sequence similarity to other protein sequences available in the NBRF data base, or from translated Genbank sequences. By hybridization of PET117 or PET191 probes first to a chromosome blot and next to a library of physically mapped fragments of yeast genomic DNA, the map locations of the PET191 and PET117 genes were determined. PET117 is located on chromosome 5 near the HIS1 gene and PET191 is located on chromosome 10 near the CYC1 gene.</description><subject>adn</subject><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Chromosomes, Fungal</subject><subject>citocromo c oxidasa</subject><subject>Cloning, Molecular</subject><subject>cytochrome c oxidase</subject><subject>cytochrome c oxydase</subject><subject>dna</subject><subject>DNA, Fungal</subject><subject>Electron Transport Complex IV - genetics</subject><subject>Electron Transport Complex IV - metabolism</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>gene</subject><subject>gene location</subject><subject>genes</subject><subject>Genes, Fungal</subject><subject>Genes. Genome</subject><subject>Genetic Complementation Test</subject><subject>localisation de gene</subject><subject>localizacion de genes</subject><subject>Membrane Proteins - genetics</subject><subject>Membrane Proteins - metabolism</subject><subject>mitochondria</subject><subject>mitochondrie</subject><subject>mitocondria</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>nucleotide sequence</subject><subject>Saccharomyces cerevisiae</subject><subject>Saccharomyces cerevisiae - genetics</subject><subject>Saccharomyces cerevisiae Proteins - genetics</subject><subject>Saccharomyces cerevisiae Proteins - metabolism</subject><subject>secuencia nucleica</subject><subject>sequence nucleique</subject><issn>0172-8083</issn><issn>1432-0983</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1vFDEQxS0ECsdBQw9yEVEgLfhzbZcQLgQpEkgJ9WrWO06MdtfB3gtcJP53DHcKJdUU7_feaOYR8pyzN5wx8_b9KWNStkaJB2TFlRQNc1Y-JCvGjWgss_IxeVLKN8a4sM4ckSMrLZfSrMivC_y-xdkjhXmg_jqnKZU0wUjH5GGMd7DENNMU6PIj0S-bS3qFMxaaqy1mHGhImfrdkv5akXqafsYBSs0rBad-3NE40wvw_hoqsPPV6zHjbSwR8Cl5FGAs-Oww1-Tr6eby5Kw5__zx08m788YLy5bG9UJKzlVopVBWiZ63dlCD4kHrYJ1uJSCHvh7qnR4stIi9R26kd86FtpVr8mqfe5NTPbcs3RSLx3GEGdO2dEZrK2Vd8j-Qt1paXck1eb0HfU6lZAzdTY4T5F3HWfenlO5fKRV-cUjd9hMO9-ihhaofH3Qo9ekhw-xjuceUFkIzXrGXeyxA6uAqV-TDhjunGHNGOCN_A6eynUQ</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>MCEWEN, J. E</creator><creator>HERAN HONG, K</creator><creator>PARK, S</creator><creator>PRECIADO, G. T</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>8FD</scope><scope>FR3</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Sequence and chromosomal localization of two PET genes required for cytochrome c oxidase assembly in Saccharomyces cerevisiae</title><author>MCEWEN, J. E ; HERAN HONG, K ; PARK, S ; PRECIADO, G. T</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c280t-9b233114f6324842b168d4d41f55f89563ae1ab808c95d8a6eebce173c999f663</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>adn</topic><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Chromosomes, Fungal</topic><topic>citocromo c oxidasa</topic><topic>Cloning, Molecular</topic><topic>cytochrome c oxidase</topic><topic>cytochrome c oxydase</topic><topic>dna</topic><topic>DNA, Fungal</topic><topic>Electron Transport Complex IV - genetics</topic><topic>Electron Transport Complex IV - metabolism</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>gene</topic><topic>gene location</topic><topic>genes</topic><topic>Genes, Fungal</topic><topic>Genes. Genome</topic><topic>Genetic Complementation Test</topic><topic>localisation de gene</topic><topic>localizacion de genes</topic><topic>Membrane Proteins - genetics</topic><topic>Membrane Proteins - metabolism</topic><topic>mitochondria</topic><topic>mitochondrie</topic><topic>mitocondria</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>nucleotide sequence</topic><topic>Saccharomyces cerevisiae</topic><topic>Saccharomyces cerevisiae - genetics</topic><topic>Saccharomyces cerevisiae Proteins - genetics</topic><topic>Saccharomyces cerevisiae Proteins - metabolism</topic><topic>secuencia nucleica</topic><topic>sequence nucleique</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>MCEWEN, J. E</creatorcontrib><creatorcontrib>HERAN HONG, K</creatorcontrib><creatorcontrib>PARK, S</creatorcontrib><creatorcontrib>PRECIADO, G. T</creatorcontrib><creatorcontrib>California Univ., Los Angeles (USA). Dept. of Microbiology. Molecular Biology Inst</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Current genetics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>MCEWEN, J. E</au><au>HERAN HONG, K</au><au>PARK, S</au><au>PRECIADO, G. T</au><aucorp>California Univ., Los Angeles (USA). Dept. of Microbiology. Molecular Biology Inst</aucorp><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Sequence and chromosomal localization of two PET genes required for cytochrome c oxidase assembly in Saccharomyces cerevisiae</atitle><jtitle>Current genetics</jtitle><addtitle>Curr Genet</addtitle><date>1993</date><risdate>1993</risdate><volume>23</volume><issue>1</issue><spage>9</spage><epage>14</epage><pages>9-14</pages><issn>0172-8083</issn><eissn>1432-0983</eissn><coden>CUGED5</coden><abstract>The nuclear genes PET117 and PET191 are required for the assembly of active cytochrome c oxidase in Saccharomyces cerevisiae, yet their gene products are not subunits of the final assembled cytochrome c oxidase complex. Plasmids bearing PET117 or PET191 were isolated by their ability to complement the pet117-1 or pet191-1 mutations, respectively. By restriction mapping, subcloning, and deletion analysis of yeast DNA fragments that complement these mutations, the PET117 and PET191 genes were localized to smaller regions of DNA, which were then sequenced from both strands. The PET117 open reading frame is of 107 codons and the PET191 open reading frame is of 108 codons. Neither the PET191 nor PET117 DNA sequences have been reported previously, and the derived amino-acid sequences of the PET191 and PET117 open reading frames exhibit no significant primary amino-acid sequence similarity to other protein sequences available in the NBRF data base, or from translated Genbank sequences. By hybridization of PET117 or PET191 probes first to a chromosome blot and next to a library of physically mapped fragments of yeast genomic DNA, the map locations of the PET191 and PET117 genes were determined. PET117 is located on chromosome 5 near the HIS1 gene and PET191 is located on chromosome 10 near the CYC1 gene.</abstract><cop>Heidelberg</cop><cop>Berlin</cop><pub>Springer</pub><pmid>8381337</pmid><doi>10.1007/BF00336742</doi><tpages>6</tpages></addata></record> |
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| subjects | adn Amino Acid Sequence Base Sequence Biological and medical sciences Chromosomes, Fungal citocromo c oxidasa Cloning, Molecular cytochrome c oxidase cytochrome c oxydase dna DNA, Fungal Electron Transport Complex IV - genetics Electron Transport Complex IV - metabolism Fundamental and applied biological sciences. Psychology gene gene location genes Genes, Fungal Genes. Genome Genetic Complementation Test localisation de gene localizacion de genes Membrane Proteins - genetics Membrane Proteins - metabolism mitochondria mitochondrie mitocondria Molecular and cellular biology Molecular genetics Molecular Sequence Data nucleotide sequence Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics Saccharomyces cerevisiae Proteins - genetics Saccharomyces cerevisiae Proteins - metabolism secuencia nucleica sequence nucleique |
| title | Sequence and chromosomal localization of two PET genes required for cytochrome c oxidase assembly in Saccharomyces cerevisiae |
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