Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine
The goal of our experiments was to determine the extent to which the humoral response to a melanoma vaccine elicits the production of cytotoxic antibodies in tumor‐challenged mice. Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from B 16 F 10 mela...
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| Veröffentlicht in: | International journal of cancer 1993-02, Vol.53 (4), p.696-702 |
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| container_title | International journal of cancer |
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| creator | Shrayer, David Kouttab, Nicolas Maizel, Abby Wanebo, Harold Hearing, Vincent J. Gersten, Douglas M. |
| description | The goal of our experiments was to determine the extent to which the humoral response to a melanoma vaccine elicits the production of cytotoxic antibodies in tumor‐challenged mice. Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from B 16 F 10 melanomas. The production of antibodies that recognized the vaccine preparation was determined by ELISA, as was their cross‐reactivity with the B700 melanoma,antigen. The antibodies were shown to be anti‐proliferative by inhibition of tritiated thymidine incorporation into the DNA of cultured target cells and cytotoxic by assays for complement‐mediated and antibodydependent cellular cytotoxicity. Flow cytometric analyses indicated that‐60% of the target cells specifically bound antibody from the immune sera. These results confirm that B700 is a significant antigenic component of the FECA vaccine, and provide encouragement for this approach to developing useful melanoma vaccines. |
| doi_str_mv | 10.1002/ijc.2910530428 |
| format | Article |
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Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from B 16 F 10 melanomas. The production of antibodies that recognized the vaccine preparation was determined by ELISA, as was their cross‐reactivity with the B700 melanoma,antigen. The antibodies were shown to be anti‐proliferative by inhibition of tritiated thymidine incorporation into the DNA of cultured target cells and cytotoxic by assays for complement‐mediated and antibodydependent cellular cytotoxicity. Flow cytometric analyses indicated that‐60% of the target cells specifically bound antibody from the immune sera. These results confirm that B700 is a significant antigenic component of the FECA vaccine, and provide encouragement for this approach to developing useful melanoma vaccines.</description><identifier>ISSN: 0020-7136</identifier><identifier>EISSN: 1097-0215</identifier><identifier>DOI: 10.1002/ijc.2910530428</identifier><identifier>PMID: 8436442</identifier><identifier>CODEN: IJCNAW</identifier><language>eng</language><publisher>New York: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Antibodies, Neoplasm - immunology ; Antigens, Neoplasm - chemistry ; Antigens, Neoplasm - immunology ; Antigens, Surface - chemistry ; Antigens, Surface - immunology ; Antineoplastic agents ; Biological and medical sciences ; Cytotoxicity, Immunologic ; Fibrosarcoma - immunology ; Formaldehyde ; Immunotherapy ; Medical sciences ; Melanoma, Experimental - immunology ; Mice ; Mice, Inbred C57BL ; Molecular Weight ; Neoplasm Proteins - chemistry ; Neoplasm Proteins - immunology ; Pharmacology. Drug treatments ; Tumor Cells, Cultured ; Vaccines - immunology</subject><ispartof>International journal of cancer, 1993-02, Vol.53 (4), p.696-702</ispartof><rights>Copyright © 1993 Wiley‐Liss, Inc., A Wiley Company</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3698-8465dbc847a1cceefeff5b77368719bdb0f6e0c9f393d70c80e0202cf18a97623</citedby><cites>FETCH-LOGICAL-c3698-8465dbc847a1cceefeff5b77368719bdb0f6e0c9f393d70c80e0202cf18a97623</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fijc.2910530428$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fijc.2910530428$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>315,781,785,1418,27929,27930,45579,45580</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4627281$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8436442$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Shrayer, David</creatorcontrib><creatorcontrib>Kouttab, Nicolas</creatorcontrib><creatorcontrib>Maizel, Abby</creatorcontrib><creatorcontrib>Wanebo, Harold</creatorcontrib><creatorcontrib>Hearing, Vincent J.</creatorcontrib><creatorcontrib>Gersten, Douglas M.</creatorcontrib><title>Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine</title><title>International journal of cancer</title><addtitle>Int J Cancer</addtitle><description>The goal of our experiments was to determine the extent to which the humoral response to a melanoma vaccine elicits the production of cytotoxic antibodies in tumor‐challenged mice. Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from B 16 F 10 melanomas. The production of antibodies that recognized the vaccine preparation was determined by ELISA, as was their cross‐reactivity with the B700 melanoma,antigen. The antibodies were shown to be anti‐proliferative by inhibition of tritiated thymidine incorporation into the DNA of cultured target cells and cytotoxic by assays for complement‐mediated and antibodydependent cellular cytotoxicity. Flow cytometric analyses indicated that‐60% of the target cells specifically bound antibody from the immune sera. These results confirm that B700 is a significant antigenic component of the FECA vaccine, and provide encouragement for this approach to developing useful melanoma vaccines.</description><subject>Animals</subject><subject>Antibodies, Neoplasm - immunology</subject><subject>Antigens, Neoplasm - chemistry</subject><subject>Antigens, Neoplasm - immunology</subject><subject>Antigens, Surface - chemistry</subject><subject>Antigens, Surface - immunology</subject><subject>Antineoplastic agents</subject><subject>Biological and medical sciences</subject><subject>Cytotoxicity, Immunologic</subject><subject>Fibrosarcoma - immunology</subject><subject>Formaldehyde</subject><subject>Immunotherapy</subject><subject>Medical sciences</subject><subject>Melanoma, Experimental - immunology</subject><subject>Mice</subject><subject>Mice, Inbred C57BL</subject><subject>Molecular Weight</subject><subject>Neoplasm Proteins - chemistry</subject><subject>Neoplasm Proteins - immunology</subject><subject>Pharmacology. Drug treatments</subject><subject>Tumor Cells, Cultured</subject><subject>Vaccines - immunology</subject><issn>0020-7136</issn><issn>1097-0215</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkM1P3DAQxS1UtN1uufZWyYeKW5axndjOsaz4qpC4wKWXyHHGrVESUzuhLH99jXZFuXGaw_vNmzePkC8M1gyAn_h7u-Y1g0pAyfUBWTKoVQGcVR_IMgNQKCbkR_IppXsAxiooF2ShSyHLki_JzwscMZrJh5EGR-12ClN48paacfJt6DwmOgU6_UZ6yiQd5uhHpAP2ZgyDoXPy4y9qqAtxML0f_TN29NFYm6nP5NCZPuHRfq7I3fnZ7eayuL65uNp8vy6skLUudCmrrrW6VIZZi-jQuapVSkitWN12LTiJYGsnatEpsBowf8WtY9rUSnKxIsc734cY_syYpmbwyWKfI2KYU6OqSnOhIYPrHWhjSCmiax6iH0zcNgyalzKbXGbzv8y88HXvPLcDdq_4vr2sf9vrJlnTu2hG69MrVkquuGYZq3fYX9_j9p2jzdWPzZsI_wBnro1h</recordid><startdate>19930220</startdate><enddate>19930220</enddate><creator>Shrayer, David</creator><creator>Kouttab, Nicolas</creator><creator>Maizel, Abby</creator><creator>Wanebo, Harold</creator><creator>Hearing, Vincent J.</creator><creator>Gersten, Douglas M.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley-Liss</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930220</creationdate><title>Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine</title><author>Shrayer, David ; Kouttab, Nicolas ; Maizel, Abby ; Wanebo, Harold ; Hearing, Vincent J. ; Gersten, Douglas M.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3698-8465dbc847a1cceefeff5b77368719bdb0f6e0c9f393d70c80e0202cf18a97623</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Antibodies, Neoplasm - immunology</topic><topic>Antigens, Neoplasm - chemistry</topic><topic>Antigens, Neoplasm - immunology</topic><topic>Antigens, Surface - chemistry</topic><topic>Antigens, Surface - immunology</topic><topic>Antineoplastic agents</topic><topic>Biological and medical sciences</topic><topic>Cytotoxicity, Immunologic</topic><topic>Fibrosarcoma - immunology</topic><topic>Formaldehyde</topic><topic>Immunotherapy</topic><topic>Medical sciences</topic><topic>Melanoma, Experimental - immunology</topic><topic>Mice</topic><topic>Mice, Inbred C57BL</topic><topic>Molecular Weight</topic><topic>Neoplasm Proteins - chemistry</topic><topic>Neoplasm Proteins - immunology</topic><topic>Pharmacology. Drug treatments</topic><topic>Tumor Cells, Cultured</topic><topic>Vaccines - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Shrayer, David</creatorcontrib><creatorcontrib>Kouttab, Nicolas</creatorcontrib><creatorcontrib>Maizel, Abby</creatorcontrib><creatorcontrib>Wanebo, Harold</creatorcontrib><creatorcontrib>Hearing, Vincent J.</creatorcontrib><creatorcontrib>Gersten, Douglas M.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>International journal of cancer</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Shrayer, David</au><au>Kouttab, Nicolas</au><au>Maizel, Abby</au><au>Wanebo, Harold</au><au>Hearing, Vincent J.</au><au>Gersten, Douglas M.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine</atitle><jtitle>International journal of cancer</jtitle><addtitle>Int J Cancer</addtitle><date>1993-02-20</date><risdate>1993</risdate><volume>53</volume><issue>4</issue><spage>696</spage><epage>702</epage><pages>696-702</pages><issn>0020-7136</issn><eissn>1097-0215</eissn><coden>IJCNAW</coden><abstract>The goal of our experiments was to determine the extent to which the humoral response to a melanoma vaccine elicits the production of cytotoxic antibodies in tumor‐challenged mice. Mice were immunized with a vaccine produced from formalinized extracellular antigens (FECA) derived from B 16 F 10 melanomas. The production of antibodies that recognized the vaccine preparation was determined by ELISA, as was their cross‐reactivity with the B700 melanoma,antigen. The antibodies were shown to be anti‐proliferative by inhibition of tritiated thymidine incorporation into the DNA of cultured target cells and cytotoxic by assays for complement‐mediated and antibodydependent cellular cytotoxicity. Flow cytometric analyses indicated that‐60% of the target cells specifically bound antibody from the immune sera. These results confirm that B700 is a significant antigenic component of the FECA vaccine, and provide encouragement for this approach to developing useful melanoma vaccines.</abstract><cop>New York</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8436442</pmid><doi>10.1002/ijc.2910530428</doi><tpages>7</tpages></addata></record> |
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| ispartof | International journal of cancer, 1993-02, Vol.53 (4), p.696-702 |
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| language | eng |
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| subjects | Animals Antibodies, Neoplasm - immunology Antigens, Neoplasm - chemistry Antigens, Neoplasm - immunology Antigens, Surface - chemistry Antigens, Surface - immunology Antineoplastic agents Biological and medical sciences Cytotoxicity, Immunologic Fibrosarcoma - immunology Formaldehyde Immunotherapy Medical sciences Melanoma, Experimental - immunology Mice Mice, Inbred C57BL Molecular Weight Neoplasm Proteins - chemistry Neoplasm Proteins - immunology Pharmacology. Drug treatments Tumor Cells, Cultured Vaccines - immunology |
| title | Generation of cytotoxic antibodies to the B16 murine melanoma using a formalinized vaccine |
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