High-level expression of recombinant human soluble thrombomodulin in serum-free medium by CHO-K1 cells

Cultivation of gene-engineered Chinese hamster ovary (CHO-K1) cells that produce recombinant human soluble thrombomodulin (rsTM) was investigated to optimize conditions for high-level expression of the protein in a serum-free medium. For economic protein production, oxygenation of cultures with pure...

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Veröffentlicht in:	Applied microbiology and biotechnology 1993, Vol.38 (4), p.520-525
Hauptverfasser:	OGATA, M, WAKITA, K.-I, KIMURA, K, MARUMOTO, Y, OH-I, K, SHIMIZU, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Biological and medical sciences Biotechnology Cell Division CHO Cells Cloning, Molecular Cricetinae Culture Media, Serum-Free - pharmacology Fundamental and applied biological sciences. Psychology Genetic engineering Genetic technics Glucose - pharmacology Humans Methods. Procedures. Technologies Microspheres Modification of gene expression level Oxygen - pharmacology Receptors, Cell Surface - biosynthesis Receptors, Cell Surface - genetics Receptors, Thrombin Recombinant Proteins - biosynthesis Recombinant Proteins - genetics Time Factors
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