Molecular cloning and characterization of anther-preferential cDNA encoding a putative actin-depolymerizing factor
A cDNA clone, LMP131A, which is preferentially expressed in mature anther was isolated from a lily cDNA library. Northern blot analysis and plaque hybridization experiments showed that the LMP131A mRNA is present at ca. 0.3% of the mRNA in mature pollen and is not detectable in carpel, petal, floral...
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| Veröffentlicht in: | Plant molecular biology 1993-01, Vol.21 (1), p.39-45 |
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| creator | Kim, S.R. (Washington State Univ., Pullman, WA (USA). Inst. of Biological Chemistry) Kim, Y An, G |
| description | A cDNA clone, LMP131A, which is preferentially expressed in mature anther was isolated from a lily cDNA library. Northern blot analysis and plaque hybridization experiments showed that the LMP131A mRNA is present at ca. 0.3% of the mRNA in mature pollen and is not detectable in carpel, petal, floral bud, leaf, or root. The clone contains an open reading frame of 139 amino acid residues which shows greater than 40% sequence identity in a 91 amino acid overlap to animal actin-depolymerizing factors (ADF), cofilin and destrin. The sequences at and near the actin-binding site are most conserved. Using the lily clone as a probe, a cDNA clone, BMP1, was isolated from a mature anther library of Brassica napus. The expression pattern of the BMP1 clone was the same as that of the lily clone. The Brassica anther-preferential clone contains an open reading frame which is 79% identical to the lily LMP131A protein. Southern blot analysis showed that there are one or a few copies of the putative ADF genes in B. napus and Arabidopsis thaliana. |
| doi_str_mv | 10.1007/bf00039616 |
| format | Article |
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(Washington State Univ., Pullman, WA (USA). Inst. of Biological Chemistry) ; Kim, Y ; An, G</creator><creatorcontrib>Kim, S.R. (Washington State Univ., Pullman, WA (USA). Inst. of Biological Chemistry) ; Kim, Y ; An, G</creatorcontrib><description>A cDNA clone, LMP131A, which is preferentially expressed in mature anther was isolated from a lily cDNA library. Northern blot analysis and plaque hybridization experiments showed that the LMP131A mRNA is present at ca. 0.3% of the mRNA in mature pollen and is not detectable in carpel, petal, floral bud, leaf, or root. The clone contains an open reading frame of 139 amino acid residues which shows greater than 40% sequence identity in a 91 amino acid overlap to animal actin-depolymerizing factors (ADF), cofilin and destrin. The sequences at and near the actin-binding site are most conserved. Using the lily clone as a probe, a cDNA clone, BMP1, was isolated from a mature anther library of Brassica napus. The expression pattern of the BMP1 clone was the same as that of the lily clone. The Brassica anther-preferential clone contains an open reading frame which is 79% identical to the lily LMP131A protein. Southern blot analysis showed that there are one or a few copies of the putative ADF genes in B. napus and Arabidopsis thaliana.</description><identifier>ISSN: 0167-4412</identifier><identifier>EISSN: 1573-5028</identifier><identifier>DOI: 10.1007/bf00039616</identifier><identifier>PMID: 8425049</identifier><identifier>CODEN: PMBIDB</identifier><language>eng</language><publisher>Dordrecht: Springer</publisher><subject>Actin Depolymerizing Factors ; Actins - metabolism ; ADN ; Amino Acid Sequence ; Animals ; Base Sequence ; Biological and medical sciences ; Blotting, Northern ; BRASSICA NAPUS ; CLONACION MOLECULAR ; CLONAGE MOLECULAIRE ; Cloning, Molecular - methods ; CODE GENETIQUE ; CODIGO GENETICO ; Destrin ; DNA ; DNA - genetics ; EXPRESION GENICA ; EXPRESSION DES GENES ; Fundamental and applied biological sciences. Psychology ; GENE EXPRESSION ; Gene Library ; Genes. Genome ; GENETIC CODE ; Humans ; LILIUM ; Microfilament Proteins - genetics ; Molecular and cellular biology ; MOLECULAR CLONING ; Molecular genetics ; Molecular Sequence Data ; Plants - genetics ; RNA, Messenger - genetics ; RNA, Messenger - metabolism ; Sequence Homology, Amino Acid</subject><ispartof>Plant molecular biology, 1993-01, Vol.21 (1), p.39-45</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c427t-41f3fc89782eaa8d8a543b537fcdeb6c7dd8898e985b829c62bad73720d10dca3</citedby><cites>FETCH-LOGICAL-c427t-41f3fc89782eaa8d8a543b537fcdeb6c7dd8898e985b829c62bad73720d10dca3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,4010,27900,27901,27902</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4603381$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8425049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kim, S.R. (Washington State Univ., Pullman, WA (USA). Inst. of Biological Chemistry)</creatorcontrib><creatorcontrib>Kim, Y</creatorcontrib><creatorcontrib>An, G</creatorcontrib><title>Molecular cloning and characterization of anther-preferential cDNA encoding a putative actin-depolymerizing factor</title><title>Plant molecular biology</title><addtitle>Plant Mol Biol</addtitle><description>A cDNA clone, LMP131A, which is preferentially expressed in mature anther was isolated from a lily cDNA library. Northern blot analysis and plaque hybridization experiments showed that the LMP131A mRNA is present at ca. 0.3% of the mRNA in mature pollen and is not detectable in carpel, petal, floral bud, leaf, or root. The clone contains an open reading frame of 139 amino acid residues which shows greater than 40% sequence identity in a 91 amino acid overlap to animal actin-depolymerizing factors (ADF), cofilin and destrin. The sequences at and near the actin-binding site are most conserved. Using the lily clone as a probe, a cDNA clone, BMP1, was isolated from a mature anther library of Brassica napus. The expression pattern of the BMP1 clone was the same as that of the lily clone. The Brassica anther-preferential clone contains an open reading frame which is 79% identical to the lily LMP131A protein. Southern blot analysis showed that there are one or a few copies of the putative ADF genes in B. napus and Arabidopsis thaliana.</description><subject>Actin Depolymerizing Factors</subject><subject>Actins - metabolism</subject><subject>ADN</subject><subject>Amino Acid Sequence</subject><subject>Animals</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Blotting, Northern</subject><subject>BRASSICA NAPUS</subject><subject>CLONACION MOLECULAR</subject><subject>CLONAGE MOLECULAIRE</subject><subject>Cloning, Molecular - methods</subject><subject>CODE GENETIQUE</subject><subject>CODIGO GENETICO</subject><subject>Destrin</subject><subject>DNA</subject><subject>DNA - genetics</subject><subject>EXPRESION GENICA</subject><subject>EXPRESSION DES GENES</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>GENE EXPRESSION</subject><subject>Gene Library</subject><subject>Genes. Genome</subject><subject>GENETIC CODE</subject><subject>Humans</subject><subject>LILIUM</subject><subject>Microfilament Proteins - genetics</subject><subject>Molecular and cellular biology</subject><subject>MOLECULAR CLONING</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Plants - genetics</subject><subject>RNA, Messenger - genetics</subject><subject>RNA, Messenger - metabolism</subject><subject>Sequence Homology, Amino Acid</subject><issn>0167-4412</issn><issn>1573-5028</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0btvFDEQB2ALgZJLoKFEirQFShFpwa_1o8wTkI7QQL2a9SPZyGcv9i5S-Ovx5S5pqUaa3zdTzCD0nuBPBGP5efAYY6YFEa_QinSStR2m6jVaYSJkyzmhh-iolAeMK2fiAB0oTjvM9Qrl7yk4swTIjQkpjvGugWgbcw8ZzOzy-BfmMcUm-dqf711up-y8yy7OI4TGXN2eNy6aZJ8mm2mZq__jmjo8xta6KYXHzXbNNve1m_Jb9MZDKO7dvh6jXzfXPy-_tusfX75dnq9bw6mcW04880ZpqagDUFZBx9nQMemNdYMw0lqltHJadYOi2gg6gJVMUmwJtgbYMTrd7Z1y-r24MvebsRgXAkSXltLLrpNaCPpfSCoikukKz3bQ5FRKvUM_5XED-bEnuN9-or-4ef5ExSf7rcuwcfaF7k9f84_7HIqB4DNEM5YXxgVmTJHKPuyYh9TDXa7kdq0ZJoQr9g-DMpoC</recordid><startdate>199301</startdate><enddate>199301</enddate><creator>Kim, S.R. (Washington State Univ., Pullman, WA (USA). Inst. of Biological Chemistry)</creator><creator>Kim, Y</creator><creator>An, G</creator><general>Springer</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TM</scope><scope>8FD</scope><scope>FR3</scope><scope>P64</scope><scope>RC3</scope><scope>7X8</scope></search><sort><creationdate>199301</creationdate><title>Molecular cloning and characterization of anther-preferential cDNA encoding a putative actin-depolymerizing factor</title><author>Kim, S.R. (Washington State Univ., Pullman, WA (USA). Inst. of Biological Chemistry) ; Kim, Y ; An, G</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c427t-41f3fc89782eaa8d8a543b537fcdeb6c7dd8898e985b829c62bad73720d10dca3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Actin Depolymerizing Factors</topic><topic>Actins - metabolism</topic><topic>ADN</topic><topic>Amino Acid Sequence</topic><topic>Animals</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Blotting, Northern</topic><topic>BRASSICA NAPUS</topic><topic>CLONACION MOLECULAR</topic><topic>CLONAGE MOLECULAIRE</topic><topic>Cloning, Molecular - methods</topic><topic>CODE GENETIQUE</topic><topic>CODIGO GENETICO</topic><topic>Destrin</topic><topic>DNA</topic><topic>DNA - genetics</topic><topic>EXPRESION GENICA</topic><topic>EXPRESSION DES GENES</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>GENE EXPRESSION</topic><topic>Gene Library</topic><topic>Genes. Genome</topic><topic>GENETIC CODE</topic><topic>Humans</topic><topic>LILIUM</topic><topic>Microfilament Proteins - genetics</topic><topic>Molecular and cellular biology</topic><topic>MOLECULAR CLONING</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Plants - genetics</topic><topic>RNA, Messenger - genetics</topic><topic>RNA, Messenger - metabolism</topic><topic>Sequence Homology, Amino Acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kim, S.R. (Washington State Univ., Pullman, WA (USA). Inst. of Biological Chemistry)</creatorcontrib><creatorcontrib>Kim, Y</creatorcontrib><creatorcontrib>An, G</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Nucleic Acids Abstracts</collection><collection>Technology Research Database</collection><collection>Engineering Research Database</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Plant molecular biology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kim, S.R. (Washington State Univ., Pullman, WA (USA). Inst. of Biological Chemistry)</au><au>Kim, Y</au><au>An, G</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular cloning and characterization of anther-preferential cDNA encoding a putative actin-depolymerizing factor</atitle><jtitle>Plant molecular biology</jtitle><addtitle>Plant Mol Biol</addtitle><date>1993-01</date><risdate>1993</risdate><volume>21</volume><issue>1</issue><spage>39</spage><epage>45</epage><pages>39-45</pages><issn>0167-4412</issn><eissn>1573-5028</eissn><coden>PMBIDB</coden><abstract>A cDNA clone, LMP131A, which is preferentially expressed in mature anther was isolated from a lily cDNA library. Northern blot analysis and plaque hybridization experiments showed that the LMP131A mRNA is present at ca. 0.3% of the mRNA in mature pollen and is not detectable in carpel, petal, floral bud, leaf, or root. The clone contains an open reading frame of 139 amino acid residues which shows greater than 40% sequence identity in a 91 amino acid overlap to animal actin-depolymerizing factors (ADF), cofilin and destrin. The sequences at and near the actin-binding site are most conserved. Using the lily clone as a probe, a cDNA clone, BMP1, was isolated from a mature anther library of Brassica napus. The expression pattern of the BMP1 clone was the same as that of the lily clone. The Brassica anther-preferential clone contains an open reading frame which is 79% identical to the lily LMP131A protein. Southern blot analysis showed that there are one or a few copies of the putative ADF genes in B. napus and Arabidopsis thaliana.</abstract><cop>Dordrecht</cop><pub>Springer</pub><pmid>8425049</pmid><doi>10.1007/bf00039616</doi><tpages>7</tpages></addata></record> |
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| subjects | Actin Depolymerizing Factors Actins - metabolism ADN Amino Acid Sequence Animals Base Sequence Biological and medical sciences Blotting, Northern BRASSICA NAPUS CLONACION MOLECULAR CLONAGE MOLECULAIRE Cloning, Molecular - methods CODE GENETIQUE CODIGO GENETICO Destrin DNA DNA - genetics EXPRESION GENICA EXPRESSION DES GENES Fundamental and applied biological sciences. Psychology GENE EXPRESSION Gene Library Genes. Genome GENETIC CODE Humans LILIUM Microfilament Proteins - genetics Molecular and cellular biology MOLECULAR CLONING Molecular genetics Molecular Sequence Data Plants - genetics RNA, Messenger - genetics RNA, Messenger - metabolism Sequence Homology, Amino Acid |
| title | Molecular cloning and characterization of anther-preferential cDNA encoding a putative actin-depolymerizing factor |
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