Linkage of human spermatid-specific basic nuclear protein genes. Definition and evolution of the P1-->P2-->TP2 locus
Protamines and transition proteins are highly basic sperm-specific nuclear proteins that serve to compact the DNA within the condensing spermatid nucleus. It has been proposed that the genes for protamine P1 and P2 and transition protein 2 may be clustered at a single loci. This may have marked impl...
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| Veröffentlicht in: | The Journal of biological chemistry 1993-02, Vol.268 (4), p.2932-2936 |
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| container_title | The Journal of biological chemistry |
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| creator | NELSON, J. E KRAWETZ, S. A |
| description | Protamines and transition proteins are highly basic sperm-specific nuclear proteins that serve to compact the DNA within the
condensing spermatid nucleus. It has been proposed that the genes for protamine P1 and P2 and transition protein 2 may be
clustered at a single loci. This may have marked implications for their evolution and coordinate developmental regulation.
The cloning of a contiguous segment encompassing this region would directly address these issues. We have utilized a cloned
segment of the human protamine P1 gene to isolate a human cosmid clone which contains the genes for protamines P1 and P2 and
transition protein TP2. Sequence and hybridization analysis of this cosmid showed that the TP2 gene resided 3' of the P2 gene
at a distance of approximately 7 kilobases (kb). This establishes the order of this gene cluster within a 13-kb region of
16p13.13-16p13.2 to be P1-->P2-->TP2. Similar to their spatial order, comparative computer-assisted sequence analysis predicts
an identical relative rank order of emergence. Accordingly we propose that this gene cluster may have arisen from the P1 gene
through a series of gene duplication events. |
| doi_str_mv | 10.1016/S0021-9258(18)53863-1 |
| format | Article |
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condensing spermatid nucleus. It has been proposed that the genes for protamine P1 and P2 and transition protein 2 may be
clustered at a single loci. This may have marked implications for their evolution and coordinate developmental regulation.
The cloning of a contiguous segment encompassing this region would directly address these issues. We have utilized a cloned
segment of the human protamine P1 gene to isolate a human cosmid clone which contains the genes for protamines P1 and P2 and
transition protein TP2. Sequence and hybridization analysis of this cosmid showed that the TP2 gene resided 3' of the P2 gene
at a distance of approximately 7 kilobases (kb). This establishes the order of this gene cluster within a 13-kb region of
16p13.13-16p13.2 to be P1-->P2-->TP2. Similar to their spatial order, comparative computer-assisted sequence analysis predicts
an identical relative rank order of emergence. Accordingly we propose that this gene cluster may have arisen from the P1 gene
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condensing spermatid nucleus. It has been proposed that the genes for protamine P1 and P2 and transition protein 2 may be
clustered at a single loci. This may have marked implications for their evolution and coordinate developmental regulation.
The cloning of a contiguous segment encompassing this region would directly address these issues. We have utilized a cloned
segment of the human protamine P1 gene to isolate a human cosmid clone which contains the genes for protamines P1 and P2 and
transition protein TP2. Sequence and hybridization analysis of this cosmid showed that the TP2 gene resided 3' of the P2 gene
at a distance of approximately 7 kilobases (kb). This establishes the order of this gene cluster within a 13-kb region of
16p13.13-16p13.2 to be P1-->P2-->TP2. Similar to their spatial order, comparative computer-assisted sequence analysis predicts
an identical relative rank order of emergence. Accordingly we propose that this gene cluster may have arisen from the P1 gene
through a series of gene duplication events.</description><subject>Amino Acid Sequence</subject><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>Biological Evolution</subject><subject>Chromosomal Proteins, Non-Histone - genetics</subject><subject>Chromosomes, Human, Pair 16</subject><subject>Cloning, Molecular</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genes</subject><subject>Genes. Genome</subject><subject>Genetic Linkage</subject><subject>Humans</subject><subject>Male</subject><subject>Molecular and cellular biology</subject><subject>Molecular genetics</subject><subject>Molecular Sequence Data</subject><subject>Multigene Family</subject><subject>Nuclear Proteins - genetics</subject><subject>Restriction Mapping</subject><subject>Sequence Homology, Amino Acid</subject><subject>Spermatids - physiology</subject><issn>0021-9258</issn><issn>1083-351X</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo9kG2L1DAQgIMo597pTzgIIqIfcmby0qZfBDnPF1hwwRP8FtLsZBtt0zVpFf-9vd1l82EyYZ6ZCQ8h18BvgEP19hvnAlgjtHkN5o2WppIMHpEVcCOZ1PDjMVmdkafkspSffDmqgQtyYZQwTVWvyLSO6ZfbIR0D7ebBJVr2mAc3xS1bMh9D9LR1ZYlp9j26TPd5nDAmusOE5YZ-wBBTnOKYqEtbin_Gfj68lolTh3QDjL3biCXcbwTtRz-XZ-RJcH3B56f7inz_eHd_-5mtv376cvt-zbzi9cSkCK1SRnujOcgQhAbQ4LTnGrjijTO-NdD4GoCHBlTLa7P1OlSyFdgaLq_Iq-Pc5cu_ZyyTHWLx2Pcu4TgXW2td60rUC6iPoM9jKRmD3ec4uPzPArcPtu3Btn1QacHYg20LS9_1acHcDrg9d530LvWXp7or3vUhu-RjOWOqUuq4_sUR6-Ku-xsz2jaOvsPBispYZUUjhfwP_EqRSg</recordid><startdate>19930205</startdate><enddate>19930205</enddate><creator>NELSON, J. E</creator><creator>KRAWETZ, S. A</creator><general>American Society for Biochemistry and Molecular Biology</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930205</creationdate><title>Linkage of human spermatid-specific basic nuclear protein genes. Definition and evolution of the P1-->P2-->TP2 locus</title><author>NELSON, J. E ; KRAWETZ, S. A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c407t-32fb4485c85013ff251151a5c0510409a8cb819c7110f914b078dc5f63b2eb803</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acid Sequence</topic><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>Biological Evolution</topic><topic>Chromosomal Proteins, Non-Histone - genetics</topic><topic>Chromosomes, Human, Pair 16</topic><topic>Cloning, Molecular</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genes</topic><topic>Genes. Genome</topic><topic>Genetic Linkage</topic><topic>Humans</topic><topic>Male</topic><topic>Molecular and cellular biology</topic><topic>Molecular genetics</topic><topic>Molecular Sequence Data</topic><topic>Multigene Family</topic><topic>Nuclear Proteins - genetics</topic><topic>Restriction Mapping</topic><topic>Sequence Homology, Amino Acid</topic><topic>Spermatids - physiology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>NELSON, J. E</creatorcontrib><creatorcontrib>KRAWETZ, S. A</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of biological chemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>NELSON, J. E</au><au>KRAWETZ, S. A</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Linkage of human spermatid-specific basic nuclear protein genes. Definition and evolution of the P1-->P2-->TP2 locus</atitle><jtitle>The Journal of biological chemistry</jtitle><addtitle>J Biol Chem</addtitle><date>1993-02-05</date><risdate>1993</risdate><volume>268</volume><issue>4</issue><spage>2932</spage><epage>2936</epage><pages>2932-2936</pages><issn>0021-9258</issn><eissn>1083-351X</eissn><coden>JBCHA3</coden><abstract>Protamines and transition proteins are highly basic sperm-specific nuclear proteins that serve to compact the DNA within the
condensing spermatid nucleus. It has been proposed that the genes for protamine P1 and P2 and transition protein 2 may be
clustered at a single loci. This may have marked implications for their evolution and coordinate developmental regulation.
The cloning of a contiguous segment encompassing this region would directly address these issues. We have utilized a cloned
segment of the human protamine P1 gene to isolate a human cosmid clone which contains the genes for protamines P1 and P2 and
transition protein TP2. Sequence and hybridization analysis of this cosmid showed that the TP2 gene resided 3' of the P2 gene
at a distance of approximately 7 kilobases (kb). This establishes the order of this gene cluster within a 13-kb region of
16p13.13-16p13.2 to be P1-->P2-->TP2. Similar to their spatial order, comparative computer-assisted sequence analysis predicts
an identical relative rank order of emergence. Accordingly we propose that this gene cluster may have arisen from the P1 gene
through a series of gene duplication events.</abstract><cop>Bethesda, MD</cop><pub>American Society for Biochemistry and Molecular Biology</pub><pmid>8428967</pmid><doi>10.1016/S0021-9258(18)53863-1</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| ispartof | The Journal of biological chemistry, 1993-02, Vol.268 (4), p.2932-2936 |
| issn | 0021-9258 1083-351X |
| language | eng |
| recordid | cdi_proquest_miscellaneous_75575627 |
| source | MEDLINE; EZB-FREE-00999 freely available EZB journals; Alma/SFX Local Collection |
| subjects | Amino Acid Sequence Base Sequence Biological and medical sciences Biological Evolution Chromosomal Proteins, Non-Histone - genetics Chromosomes, Human, Pair 16 Cloning, Molecular Fundamental and applied biological sciences. Psychology Genes Genes. Genome Genetic Linkage Humans Male Molecular and cellular biology Molecular genetics Molecular Sequence Data Multigene Family Nuclear Proteins - genetics Restriction Mapping Sequence Homology, Amino Acid Spermatids - physiology |
| title | Linkage of human spermatid-specific basic nuclear protein genes. Definition and evolution of the P1-->P2-->TP2 locus |
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