Methionine but not folinic acid or vitamin B-12 alters the frequency of neural tube defects in Axd mutant mice
The Axd (axial defects) mutation, which causes open neural tube defects (NTD) in 25-30% of d 14 mouse embryos of heterozygous (Axd/+) matings and curly tails (CT) in 31%, can be useful in studying neurovertebral morphogenesis. Current interest focuses on the role of methionine in neurulation, becaus...
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Veröffentlicht in: | The Journal of nutrition 1993-01, Vol.123 (1), p.27-34 |
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description | The Axd (axial defects) mutation, which causes open neural tube defects (NTD) in 25-30% of d 14 mouse embryos of heterozygous (Axd/+) matings and curly tails (CT) in 31%, can be useful in studying neurovertebral morphogenesis. Current interest focuses on the role of methionine in neurulation, because supplementation of dams with this essential amino acid (70 mg/kg body wt) has been shown to reduce by 41% the incidence of NTD among embryos of Axd/+ X Axd/+ crosses when administered on d 8 and 9 of pregnancy. The present experiments were undertaken to determine whether supplementation of dams with a higher dose of methionine could effect a greater level of remediation. At a dose of 180 mg/kg body wt, the amino acid produced a reduction in NTD of 47%, similar to that produced at 70 mg/kg body wt. Supplementation of dams of reciprocal matings of Axd/+ X BALB/cByJ or CF-1 mice allowed assessment of the effects of methionine in heterozygous embryos, which exhibit CT. The amino acid had no effect on Axd/+ embryos from the BALB crosses, but the frequency of CT declined significantly among embryos of the CF-1 outcrosses. Maternal supplements of folinic acid (33 mg/kg) or vitamin B-12 (330 mg/kg) did not alter the incidence of NTD or CT among Axd embryos. No difference in methionine concentration was detected in the serum of Axd/+ and +/+ dams |
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(Rutgers University, Piscataway, NJ) ; Wannberg, S.L</creator><creatorcontrib>Essien, F.B. (Rutgers University, Piscataway, NJ) ; Wannberg, S.L</creatorcontrib><description>The Axd (axial defects) mutation, which causes open neural tube defects (NTD) in 25-30% of d 14 mouse embryos of heterozygous (Axd/+) matings and curly tails (CT) in 31%, can be useful in studying neurovertebral morphogenesis. Current interest focuses on the role of methionine in neurulation, because supplementation of dams with this essential amino acid (70 mg/kg body wt) has been shown to reduce by 41% the incidence of NTD among embryos of Axd/+ X Axd/+ crosses when administered on d 8 and 9 of pregnancy. The present experiments were undertaken to determine whether supplementation of dams with a higher dose of methionine could effect a greater level of remediation. At a dose of 180 mg/kg body wt, the amino acid produced a reduction in NTD of 47%, similar to that produced at 70 mg/kg body wt. Supplementation of dams of reciprocal matings of Axd/+ X BALB/cByJ or CF-1 mice allowed assessment of the effects of methionine in heterozygous embryos, which exhibit CT. The amino acid had no effect on Axd/+ embryos from the BALB crosses, but the frequency of CT declined significantly among embryos of the CF-1 outcrosses. Maternal supplements of folinic acid (33 mg/kg) or vitamin B-12 (330 mg/kg) did not alter the incidence of NTD or CT among Axd embryos. No difference in methionine concentration was detected in the serum of Axd/+ and +/+ dams</description><identifier>ISSN: 0022-3166</identifier><identifier>EISSN: 1541-6100</identifier><identifier>DOI: 10.1093/jn/123.1.27</identifier><identifier>PMID: 8421227</identifier><identifier>CODEN: JONUAI</identifier><language>eng</language><publisher>Bethesda, MD: American Society for Nutritional Sciences</publisher><subject>Amino Acids - blood ; Animals ; Biological and medical sciences ; COMPLEXE VITAMINIQUE B ; DEFAUT ; DEFECTOS ; DIETA ; Embryo, Mammalian - drug effects ; Embryology: invertebrates and vertebrates. Teratology ; Female ; Folic Acid - administration & dosage ; Folic Acid - therapeutic use ; Fundamental and applied biological sciences. Psychology ; Injections, Intraperitoneal ; Male ; METHIONINE ; Methionine - administration & dosage ; Methionine - therapeutic use ; METIONINA ; Mice ; Mice, Inbred BALB C ; Mice, Inbred Strains ; Mice, Mutant Strains ; Molecular embryology ; Neural Tube Defects - prevention & control ; RATON ; REGIME ALIMENTAIRE ; SISTEMA NERVIOSO CENTRAL ; SOURIS ; SYSTEME NERVEUX CENTRAL ; Vitamin B 12 - administration & dosage ; Vitamin B 12 - therapeutic use ; VITAMINA B12 ; VITAMINAS B ; VITAMINE B12</subject><ispartof>The Journal of nutrition, 1993-01, Vol.123 (1), p.27-34</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c2847-a25a60a6ce42b6a4a110a579f1d589412c8e7b5f9719456fea59f21f98edf10a3</citedby><cites>FETCH-LOGICAL-c2847-a25a60a6ce42b6a4a110a579f1d589412c8e7b5f9719456fea59f21f98edf10a3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,4024,27923,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4570342$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8421227$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Essien, F.B. (Rutgers University, Piscataway, NJ)</creatorcontrib><creatorcontrib>Wannberg, S.L</creatorcontrib><title>Methionine but not folinic acid or vitamin B-12 alters the frequency of neural tube defects in Axd mutant mice</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>The Axd (axial defects) mutation, which causes open neural tube defects (NTD) in 25-30% of d 14 mouse embryos of heterozygous (Axd/+) matings and curly tails (CT) in 31%, can be useful in studying neurovertebral morphogenesis. Current interest focuses on the role of methionine in neurulation, because supplementation of dams with this essential amino acid (70 mg/kg body wt) has been shown to reduce by 41% the incidence of NTD among embryos of Axd/+ X Axd/+ crosses when administered on d 8 and 9 of pregnancy. The present experiments were undertaken to determine whether supplementation of dams with a higher dose of methionine could effect a greater level of remediation. At a dose of 180 mg/kg body wt, the amino acid produced a reduction in NTD of 47%, similar to that produced at 70 mg/kg body wt. Supplementation of dams of reciprocal matings of Axd/+ X BALB/cByJ or CF-1 mice allowed assessment of the effects of methionine in heterozygous embryos, which exhibit CT. The amino acid had no effect on Axd/+ embryos from the BALB crosses, but the frequency of CT declined significantly among embryos of the CF-1 outcrosses. Maternal supplements of folinic acid (33 mg/kg) or vitamin B-12 (330 mg/kg) did not alter the incidence of NTD or CT among Axd embryos. No difference in methionine concentration was detected in the serum of Axd/+ and +/+ dams</description><subject>Amino Acids - blood</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>COMPLEXE VITAMINIQUE B</subject><subject>DEFAUT</subject><subject>DEFECTOS</subject><subject>DIETA</subject><subject>Embryo, Mammalian - drug effects</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Folic Acid - administration & dosage</subject><subject>Folic Acid - therapeutic use</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Injections, Intraperitoneal</subject><subject>Male</subject><subject>METHIONINE</subject><subject>Methionine - administration & dosage</subject><subject>Methionine - therapeutic use</subject><subject>METIONINA</subject><subject>Mice</subject><subject>Mice, Inbred BALB C</subject><subject>Mice, Inbred Strains</subject><subject>Mice, Mutant Strains</subject><subject>Molecular embryology</subject><subject>Neural Tube Defects - prevention & control</subject><subject>RATON</subject><subject>REGIME ALIMENTAIRE</subject><subject>SISTEMA NERVIOSO CENTRAL</subject><subject>SOURIS</subject><subject>SYSTEME NERVEUX CENTRAL</subject><subject>Vitamin B 12 - administration & dosage</subject><subject>Vitamin B 12 - therapeutic use</subject><subject>VITAMINA B12</subject><subject>VITAMINAS B</subject><subject>VITAMINE B12</subject><issn>0022-3166</issn><issn>1541-6100</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNo90E1v1DAQBmALgcpSOHFDQvIBcUHZehzbiY9txZdUxAF6tibOmHqVOMV2EP33BO2qpzm8z4xGL2OvQexB2PbikC5AtnvYy-4J24FW0BgQ4inbCSFl04Ixz9mLUg5CCFC2P2NnvZIgZbdj6RvVu7ikmIgPa-VpqTwsU0zRc_Rx5Evmf2LFOSZ-1YDkOFXKhdc74iHT75WSf-BL4InWjBOv60B8pEC-Fr7tXP4d-bxWTJXP0dNL9izgVOjVaZ6z208ff15_aW6-f_56fXnTeNmrrkGp0Qg0npQcDCoEEKg7G2DUvVUgfU_doIPtwCptAqG2QUKwPY1ho-05e3-8e5-X7cdS3RyLp2nCRMtaXKe1kaKFDX44Qp-XUjIFd5_jjPnBgXD_23WH5LZ2HTjZbfrt6ew6zDQ-2lOdW_7ulGPxOIWMycfyyJTuRKvkxt4cWcDF4a-8kdsftpXGGtH-A0l5iW4</recordid><startdate>199301</startdate><enddate>199301</enddate><creator>Essien, F.B. (Rutgers University, Piscataway, NJ)</creator><creator>Wannberg, S.L</creator><general>American Society for Nutritional Sciences</general><scope>FBQ</scope><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>199301</creationdate><title>Methionine but not folinic acid or vitamin B-12 alters the frequency of neural tube defects in Axd mutant mice</title><author>Essien, F.B. (Rutgers University, Piscataway, NJ) ; Wannberg, S.L</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c2847-a25a60a6ce42b6a4a110a579f1d589412c8e7b5f9719456fea59f21f98edf10a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Amino Acids - blood</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>COMPLEXE VITAMINIQUE B</topic><topic>DEFAUT</topic><topic>DEFECTOS</topic><topic>DIETA</topic><topic>Embryo, Mammalian - drug effects</topic><topic>Embryology: invertebrates and vertebrates. Teratology</topic><topic>Female</topic><topic>Folic Acid - administration & dosage</topic><topic>Folic Acid - therapeutic use</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Injections, Intraperitoneal</topic><topic>Male</topic><topic>METHIONINE</topic><topic>Methionine - administration & dosage</topic><topic>Methionine - therapeutic use</topic><topic>METIONINA</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred Strains</topic><topic>Mice, Mutant Strains</topic><topic>Molecular embryology</topic><topic>Neural Tube Defects - prevention & control</topic><topic>RATON</topic><topic>REGIME ALIMENTAIRE</topic><topic>SISTEMA NERVIOSO CENTRAL</topic><topic>SOURIS</topic><topic>SYSTEME NERVEUX CENTRAL</topic><topic>Vitamin B 12 - administration & dosage</topic><topic>Vitamin B 12 - therapeutic use</topic><topic>VITAMINA B12</topic><topic>VITAMINAS B</topic><topic>VITAMINE B12</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Essien, F.B. (Rutgers University, Piscataway, NJ)</creatorcontrib><creatorcontrib>Wannberg, S.L</creatorcontrib><collection>AGRIS</collection><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The Journal of nutrition</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Essien, F.B. (Rutgers University, Piscataway, NJ)</au><au>Wannberg, S.L</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Methionine but not folinic acid or vitamin B-12 alters the frequency of neural tube defects in Axd mutant mice</atitle><jtitle>The Journal of nutrition</jtitle><addtitle>J Nutr</addtitle><date>1993-01</date><risdate>1993</risdate><volume>123</volume><issue>1</issue><spage>27</spage><epage>34</epage><pages>27-34</pages><issn>0022-3166</issn><eissn>1541-6100</eissn><coden>JONUAI</coden><abstract>The Axd (axial defects) mutation, which causes open neural tube defects (NTD) in 25-30% of d 14 mouse embryos of heterozygous (Axd/+) matings and curly tails (CT) in 31%, can be useful in studying neurovertebral morphogenesis. Current interest focuses on the role of methionine in neurulation, because supplementation of dams with this essential amino acid (70 mg/kg body wt) has been shown to reduce by 41% the incidence of NTD among embryos of Axd/+ X Axd/+ crosses when administered on d 8 and 9 of pregnancy. The present experiments were undertaken to determine whether supplementation of dams with a higher dose of methionine could effect a greater level of remediation. At a dose of 180 mg/kg body wt, the amino acid produced a reduction in NTD of 47%, similar to that produced at 70 mg/kg body wt. Supplementation of dams of reciprocal matings of Axd/+ X BALB/cByJ or CF-1 mice allowed assessment of the effects of methionine in heterozygous embryos, which exhibit CT. The amino acid had no effect on Axd/+ embryos from the BALB crosses, but the frequency of CT declined significantly among embryos of the CF-1 outcrosses. Maternal supplements of folinic acid (33 mg/kg) or vitamin B-12 (330 mg/kg) did not alter the incidence of NTD or CT among Axd embryos. No difference in methionine concentration was detected in the serum of Axd/+ and +/+ dams</abstract><cop>Bethesda, MD</cop><pub>American Society for Nutritional Sciences</pub><pmid>8421227</pmid><doi>10.1093/jn/123.1.27</doi><tpages>8</tpages><oa>free_for_read</oa></addata></record> |
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subjects | Amino Acids - blood Animals Biological and medical sciences COMPLEXE VITAMINIQUE B DEFAUT DEFECTOS DIETA Embryo, Mammalian - drug effects Embryology: invertebrates and vertebrates. Teratology Female Folic Acid - administration & dosage Folic Acid - therapeutic use Fundamental and applied biological sciences. Psychology Injections, Intraperitoneal Male METHIONINE Methionine - administration & dosage Methionine - therapeutic use METIONINA Mice Mice, Inbred BALB C Mice, Inbred Strains Mice, Mutant Strains Molecular embryology Neural Tube Defects - prevention & control RATON REGIME ALIMENTAIRE SISTEMA NERVIOSO CENTRAL SOURIS SYSTEME NERVEUX CENTRAL Vitamin B 12 - administration & dosage Vitamin B 12 - therapeutic use VITAMINA B12 VITAMINAS B VITAMINE B12 |
title | Methionine but not folinic acid or vitamin B-12 alters the frequency of neural tube defects in Axd mutant mice |
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