Methionine but not folinic acid or vitamin B-12 alters the frequency of neural tube defects in Axd mutant mice

The Axd (axial defects) mutation, which causes open neural tube defects (NTD) in 25-30% of d 14 mouse embryos of heterozygous (Axd/+) matings and curly tails (CT) in 31%, can be useful in studying neurovertebral morphogenesis. Current interest focuses on the role of methionine in neurulation, becaus...

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Veröffentlicht in:The Journal of nutrition 1993-01, Vol.123 (1), p.27-34
Hauptverfasser: Essien, F.B. (Rutgers University, Piscataway, NJ), Wannberg, S.L
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creator Essien, F.B. (Rutgers University, Piscataway, NJ)
Wannberg, S.L
description The Axd (axial defects) mutation, which causes open neural tube defects (NTD) in 25-30% of d 14 mouse embryos of heterozygous (Axd/+) matings and curly tails (CT) in 31%, can be useful in studying neurovertebral morphogenesis. Current interest focuses on the role of methionine in neurulation, because supplementation of dams with this essential amino acid (70 mg/kg body wt) has been shown to reduce by 41% the incidence of NTD among embryos of Axd/+ X Axd/+ crosses when administered on d 8 and 9 of pregnancy. The present experiments were undertaken to determine whether supplementation of dams with a higher dose of methionine could effect a greater level of remediation. At a dose of 180 mg/kg body wt, the amino acid produced a reduction in NTD of 47%, similar to that produced at 70 mg/kg body wt. Supplementation of dams of reciprocal matings of Axd/+ X BALB/cByJ or CF-1 mice allowed assessment of the effects of methionine in heterozygous embryos, which exhibit CT. The amino acid had no effect on Axd/+ embryos from the BALB crosses, but the frequency of CT declined significantly among embryos of the CF-1 outcrosses. Maternal supplements of folinic acid (33 mg/kg) or vitamin B-12 (330 mg/kg) did not alter the incidence of NTD or CT among Axd embryos. No difference in methionine concentration was detected in the serum of Axd/+ and +/+ dams
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Supplementation of dams of reciprocal matings of Axd/+ X BALB/cByJ or CF-1 mice allowed assessment of the effects of methionine in heterozygous embryos, which exhibit CT. The amino acid had no effect on Axd/+ embryos from the BALB crosses, but the frequency of CT declined significantly among embryos of the CF-1 outcrosses. Maternal supplements of folinic acid (33 mg/kg) or vitamin B-12 (330 mg/kg) did not alter the incidence of NTD or CT among Axd embryos. 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(Rutgers University, Piscataway, NJ)</creatorcontrib><creatorcontrib>Wannberg, S.L</creatorcontrib><title>Methionine but not folinic acid or vitamin B-12 alters the frequency of neural tube defects in Axd mutant mice</title><title>The Journal of nutrition</title><addtitle>J Nutr</addtitle><description>The Axd (axial defects) mutation, which causes open neural tube defects (NTD) in 25-30% of d 14 mouse embryos of heterozygous (Axd/+) matings and curly tails (CT) in 31%, can be useful in studying neurovertebral morphogenesis. Current interest focuses on the role of methionine in neurulation, because supplementation of dams with this essential amino acid (70 mg/kg body wt) has been shown to reduce by 41% the incidence of NTD among embryos of Axd/+ X Axd/+ crosses when administered on d 8 and 9 of pregnancy. The present experiments were undertaken to determine whether supplementation of dams with a higher dose of methionine could effect a greater level of remediation. 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No difference in methionine concentration was detected in the serum of Axd/+ and +/+ dams</description><subject>Amino Acids - blood</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>COMPLEXE VITAMINIQUE B</subject><subject>DEFAUT</subject><subject>DEFECTOS</subject><subject>DIETA</subject><subject>Embryo, Mammalian - drug effects</subject><subject>Embryology: invertebrates and vertebrates. Teratology</subject><subject>Female</subject><subject>Folic Acid - administration &amp; dosage</subject><subject>Folic Acid - therapeutic use</subject><subject>Fundamental and applied biological sciences. 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Psychology</topic><topic>Injections, Intraperitoneal</topic><topic>Male</topic><topic>METHIONINE</topic><topic>Methionine - administration &amp; dosage</topic><topic>Methionine - therapeutic use</topic><topic>METIONINA</topic><topic>Mice</topic><topic>Mice, Inbred BALB C</topic><topic>Mice, Inbred Strains</topic><topic>Mice, Mutant Strains</topic><topic>Molecular embryology</topic><topic>Neural Tube Defects - prevention &amp; control</topic><topic>RATON</topic><topic>REGIME ALIMENTAIRE</topic><topic>SISTEMA NERVIOSO CENTRAL</topic><topic>SOURIS</topic><topic>SYSTEME NERVEUX CENTRAL</topic><topic>Vitamin B 12 - administration &amp; dosage</topic><topic>Vitamin B 12 - therapeutic use</topic><topic>VITAMINA B12</topic><topic>VITAMINAS B</topic><topic>VITAMINE B12</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Essien, F.B. 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subjects Amino Acids - blood
Animals
Biological and medical sciences
COMPLEXE VITAMINIQUE B
DEFAUT
DEFECTOS
DIETA
Embryo, Mammalian - drug effects
Embryology: invertebrates and vertebrates. Teratology
Female
Folic Acid - administration & dosage
Folic Acid - therapeutic use
Fundamental and applied biological sciences. Psychology
Injections, Intraperitoneal
Male
METHIONINE
Methionine - administration & dosage
Methionine - therapeutic use
METIONINA
Mice
Mice, Inbred BALB C
Mice, Inbred Strains
Mice, Mutant Strains
Molecular embryology
Neural Tube Defects - prevention & control
RATON
REGIME ALIMENTAIRE
SISTEMA NERVIOSO CENTRAL
SOURIS
SYSTEME NERVEUX CENTRAL
Vitamin B 12 - administration & dosage
Vitamin B 12 - therapeutic use
VITAMINA B12
VITAMINAS B
VITAMINE B12
title Methionine but not folinic acid or vitamin B-12 alters the frequency of neural tube defects in Axd mutant mice
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