Development of antisera selective for m4 and m5 muscarinic cholinergic receptors: distribution of m4 and m5 receptors in rat brain

A portion of the cDNA sequence corresponding to the third intracellular loop of either the m4 or m5 muscarinic cholinergic receptor was ligated into the pRIT23 or pET-3a expression vector, respectively. The expressed fusion proteins were purified and used to develop selective polyclonal antisera to...

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Veröffentlicht in:	Molecular pharmacology 1993-02, Vol.43 (2), p.149-157
Hauptverfasser:	YASUDA, R. P, CIESLA, W, FLORES, L. R, WALL, S. J, MIN LI, SATKUS, S. A, WEISSTEIN, J. S, SPAGNOLA, B. V, WOLFE, B. B
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Antibody Specificity Base Sequence Biological and medical sciences Brain Chemistry Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors CHO Cells Cricetinae Female Fundamental and applied biological sciences. Psychology Genetic Vectors Immune Sera Lung - chemistry Molecular Sequence Data Plasmids Precipitin Tests Rabbits Rats Receptors, Muscarinic - analysis Receptors, Muscarinic - biosynthesis Receptors, Muscarinic - classification Receptors, Muscarinic - immunology Recombinant Fusion Proteins - biosynthesis Tumor Cells, Cultured Vertebrates: nervous system and sense organs
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