Molecular Genetic Evidence of a Unifocal Origin for Human Serous Ovarian Carcinomas
The hypothesis that ovarian cancer is multifocal in origin was examined using molecular genetic techniques. Patterns of allelic deletion on chromosome 17 were studied in 16 informative cases of Stage III serous epithelial ovarian carcinoma. DNA was extracted from specimens collected from the omentum...
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| Veröffentlicht in: | Gynecologic oncology 1993, Vol.48 (1), p.5-10 |
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| container_title | Gynecologic oncology |
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| creator | Tsao, Sai-Wah Mok, Chi-Ho Knapp, Robert C. Oike, Kiyotaka Muto, Michael G. Welch, William R. Goodman, Howard M. Sheets, Ellen E. Berkowitz, Ross S. Lau, Ching C. |
| description | The hypothesis that ovarian cancer is multifocal in origin was examined using molecular genetic techniques. Patterns of allelic deletion on chromosome 17 were studied in 16 informative cases of Stage III serous epithelial ovarian carcinoma. DNA was extracted from specimens collected from the omentum and both ovaries, and the specific alleles and chromosomal loci involved in the deletion were identified and compared. In all cases, the patterns of allelic deletion were identical for the tumors that had been collected from different sites in the same patients. In addition, 4 of the 16 cases were heterozygous for the hypoxanthine phosphoribosyl transferase (HPRT) gene on the X-chromosome and were examined for methylation status. In all 4, the same parental allele of the HPRT gene was methylated in tumor cells collected from both ovarian and omental sites, suggesting that the patterns of inactivation of the X-chromosome are identical. This pattern of allelic deletion and HPRT-gene methylation in tumor samples collected from different sites implies that ovarian carcinomas have a unifocal origin. |
| doi_str_mv | 10.1006/gyno.1993.1002 |
| format | Article |
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Patterns of allelic deletion on chromosome 17 were studied in 16 informative cases of Stage III serous epithelial ovarian carcinoma. DNA was extracted from specimens collected from the omentum and both ovaries, and the specific alleles and chromosomal loci involved in the deletion were identified and compared. In all cases, the patterns of allelic deletion were identical for the tumors that had been collected from different sites in the same patients. In addition, 4 of the 16 cases were heterozygous for the hypoxanthine phosphoribosyl transferase (HPRT) gene on the X-chromosome and were examined for methylation status. In all 4, the same parental allele of the HPRT gene was methylated in tumor cells collected from both ovarian and omental sites, suggesting that the patterns of inactivation of the X-chromosome are identical. This pattern of allelic deletion and HPRT-gene methylation in tumor samples collected from different sites implies that ovarian carcinomas have a unifocal origin.</description><identifier>ISSN: 0090-8258</identifier><identifier>EISSN: 1095-6859</identifier><identifier>DOI: 10.1006/gyno.1993.1002</identifier><identifier>PMID: 8423021</identifier><identifier>CODEN: GYNOA3</identifier><language>eng</language><publisher>San Diego, CA: Elsevier Inc</publisher><subject>Alleles ; Biological and medical sciences ; Blotting, Southern ; Chromosomes, Human, Pair 17 - physiology ; Cystadenocarcinoma - genetics ; Cystadenocarcinoma - secondary ; DNA Probes ; Dosage Compensation, Genetic ; Female ; Female genital diseases ; Gene Deletion ; Gynecology. Andrology. Obstetrics ; Humans ; Medical sciences ; Neoplasms, Multiple Primary - genetics ; Ovarian Neoplasms - genetics ; Ovarian Neoplasms - pathology ; Tumors</subject><ispartof>Gynecologic oncology, 1993, Vol.48 (1), p.5-10</ispartof><rights>1993 Academic Press</rights><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c397t-99efcf9885a61596e4292e0ef91282d617ba0ea7bd8ee03bd4208561ca14cb7a3</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1006/gyno.1993.1002$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,4024,27923,27924,27925,45995</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4611615$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8423021$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tsao, Sai-Wah</creatorcontrib><creatorcontrib>Mok, Chi-Ho</creatorcontrib><creatorcontrib>Knapp, Robert C.</creatorcontrib><creatorcontrib>Oike, Kiyotaka</creatorcontrib><creatorcontrib>Muto, Michael G.</creatorcontrib><creatorcontrib>Welch, William R.</creatorcontrib><creatorcontrib>Goodman, Howard M.</creatorcontrib><creatorcontrib>Sheets, Ellen E.</creatorcontrib><creatorcontrib>Berkowitz, Ross S.</creatorcontrib><creatorcontrib>Lau, Ching C.</creatorcontrib><title>Molecular Genetic Evidence of a Unifocal Origin for Human Serous Ovarian Carcinomas</title><title>Gynecologic oncology</title><addtitle>Gynecol Oncol</addtitle><description>The hypothesis that ovarian cancer is multifocal in origin was examined using molecular genetic techniques. Patterns of allelic deletion on chromosome 17 were studied in 16 informative cases of Stage III serous epithelial ovarian carcinoma. DNA was extracted from specimens collected from the omentum and both ovaries, and the specific alleles and chromosomal loci involved in the deletion were identified and compared. In all cases, the patterns of allelic deletion were identical for the tumors that had been collected from different sites in the same patients. In addition, 4 of the 16 cases were heterozygous for the hypoxanthine phosphoribosyl transferase (HPRT) gene on the X-chromosome and were examined for methylation status. In all 4, the same parental allele of the HPRT gene was methylated in tumor cells collected from both ovarian and omental sites, suggesting that the patterns of inactivation of the X-chromosome are identical. This pattern of allelic deletion and HPRT-gene methylation in tumor samples collected from different sites implies that ovarian carcinomas have a unifocal origin.</description><subject>Alleles</subject><subject>Biological and medical sciences</subject><subject>Blotting, Southern</subject><subject>Chromosomes, Human, Pair 17 - physiology</subject><subject>Cystadenocarcinoma - genetics</subject><subject>Cystadenocarcinoma - secondary</subject><subject>DNA Probes</subject><subject>Dosage Compensation, Genetic</subject><subject>Female</subject><subject>Female genital diseases</subject><subject>Gene Deletion</subject><subject>Gynecology. Andrology. Obstetrics</subject><subject>Humans</subject><subject>Medical sciences</subject><subject>Neoplasms, Multiple Primary - genetics</subject><subject>Ovarian Neoplasms - genetics</subject><subject>Ovarian Neoplasms - pathology</subject><subject>Tumors</subject><issn>0090-8258</issn><issn>1095-6859</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kE1rGzEQhkVJSV23194KOoTe1h1pv6RjMWkScPAhzVnMakdBZVdKJa_B_z672OTW0_AyzwwzD2PfBGwEQPPz5RTiRmhdLlF-YCsBui4aVesrtgLQUChZq0_sc85_AaAEIa_ZtapkCVKs2NNjHMhOAyZ-R4EO3vLbo-8pWOLRceTPwbtoceD75F984C4mfj-NGPgTpThlvj9i8nPcYrI-xBHzF_bR4ZDp66Wu2fPv2z_b-2K3v3vY_toVttTtodCanHVaqRobUeuGKqklATktpJJ9I9oOgbDtekUEZddXElTdCIuisl2L5Zr9OO99TfHfRPlgRp8tDQMGmi8zbV1Xqm3kDG7OoE0x50TOvCY_YjoZAWaxaBaLZrG4xGXg-2Xz1I3Uv-MXbXP_5tLHPLtxCYP1-R2rGiHml2ZMnTGaLRw9JZOtX9T2PpE9mD76_13wBovijXA</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>Tsao, Sai-Wah</creator><creator>Mok, Chi-Ho</creator><creator>Knapp, Robert C.</creator><creator>Oike, Kiyotaka</creator><creator>Muto, Michael G.</creator><creator>Welch, William R.</creator><creator>Goodman, Howard M.</creator><creator>Sheets, Ellen E.</creator><creator>Berkowitz, Ross S.</creator><creator>Lau, Ching C.</creator><general>Elsevier Inc</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>1993</creationdate><title>Molecular Genetic Evidence of a Unifocal Origin for Human Serous Ovarian Carcinomas</title><author>Tsao, Sai-Wah ; Mok, Chi-Ho ; Knapp, Robert C. ; Oike, Kiyotaka ; Muto, Michael G. ; Welch, William R. ; Goodman, Howard M. ; Sheets, Ellen E. ; Berkowitz, Ross S. ; Lau, Ching C.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c397t-99efcf9885a61596e4292e0ef91282d617ba0ea7bd8ee03bd4208561ca14cb7a3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Alleles</topic><topic>Biological and medical sciences</topic><topic>Blotting, Southern</topic><topic>Chromosomes, Human, Pair 17 - physiology</topic><topic>Cystadenocarcinoma - genetics</topic><topic>Cystadenocarcinoma - secondary</topic><topic>DNA Probes</topic><topic>Dosage Compensation, Genetic</topic><topic>Female</topic><topic>Female genital diseases</topic><topic>Gene Deletion</topic><topic>Gynecology. Andrology. Obstetrics</topic><topic>Humans</topic><topic>Medical sciences</topic><topic>Neoplasms, Multiple Primary - genetics</topic><topic>Ovarian Neoplasms - genetics</topic><topic>Ovarian Neoplasms - pathology</topic><topic>Tumors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tsao, Sai-Wah</creatorcontrib><creatorcontrib>Mok, Chi-Ho</creatorcontrib><creatorcontrib>Knapp, Robert C.</creatorcontrib><creatorcontrib>Oike, Kiyotaka</creatorcontrib><creatorcontrib>Muto, Michael G.</creatorcontrib><creatorcontrib>Welch, William R.</creatorcontrib><creatorcontrib>Goodman, Howard M.</creatorcontrib><creatorcontrib>Sheets, Ellen E.</creatorcontrib><creatorcontrib>Berkowitz, Ross S.</creatorcontrib><creatorcontrib>Lau, Ching C.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gynecologic oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tsao, Sai-Wah</au><au>Mok, Chi-Ho</au><au>Knapp, Robert C.</au><au>Oike, Kiyotaka</au><au>Muto, Michael G.</au><au>Welch, William R.</au><au>Goodman, Howard M.</au><au>Sheets, Ellen E.</au><au>Berkowitz, Ross S.</au><au>Lau, Ching C.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Molecular Genetic Evidence of a Unifocal Origin for Human Serous Ovarian Carcinomas</atitle><jtitle>Gynecologic oncology</jtitle><addtitle>Gynecol Oncol</addtitle><date>1993</date><risdate>1993</risdate><volume>48</volume><issue>1</issue><spage>5</spage><epage>10</epage><pages>5-10</pages><issn>0090-8258</issn><eissn>1095-6859</eissn><coden>GYNOA3</coden><abstract>The hypothesis that ovarian cancer is multifocal in origin was examined using molecular genetic techniques. Patterns of allelic deletion on chromosome 17 were studied in 16 informative cases of Stage III serous epithelial ovarian carcinoma. DNA was extracted from specimens collected from the omentum and both ovaries, and the specific alleles and chromosomal loci involved in the deletion were identified and compared. In all cases, the patterns of allelic deletion were identical for the tumors that had been collected from different sites in the same patients. In addition, 4 of the 16 cases were heterozygous for the hypoxanthine phosphoribosyl transferase (HPRT) gene on the X-chromosome and were examined for methylation status. In all 4, the same parental allele of the HPRT gene was methylated in tumor cells collected from both ovarian and omental sites, suggesting that the patterns of inactivation of the X-chromosome are identical. This pattern of allelic deletion and HPRT-gene methylation in tumor samples collected from different sites implies that ovarian carcinomas have a unifocal origin.</abstract><cop>San Diego, CA</cop><pub>Elsevier Inc</pub><pmid>8423021</pmid><doi>10.1006/gyno.1993.1002</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; Elsevier ScienceDirect Journals Complete |
| subjects | Alleles Biological and medical sciences Blotting, Southern Chromosomes, Human, Pair 17 - physiology Cystadenocarcinoma - genetics Cystadenocarcinoma - secondary DNA Probes Dosage Compensation, Genetic Female Female genital diseases Gene Deletion Gynecology. Andrology. Obstetrics Humans Medical sciences Neoplasms, Multiple Primary - genetics Ovarian Neoplasms - genetics Ovarian Neoplasms - pathology Tumors |
| title | Molecular Genetic Evidence of a Unifocal Origin for Human Serous Ovarian Carcinomas |
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