Endothelial Cells Produce a Substance that Inhibits Contact Activation of Coagulation by Blocking the Activation of Hageman Factor
Human umbilical vein endothelial cells (HUVECs) produce a property that impairs the generation of coagulant and amidolytic activity initiated when normal human plasma is exposed to glass. This inhibitory property blocks the adsorption of Hageman factor (factor XII) to glass, thereby preventing the a...
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| Veröffentlicht in: | Proceedings of the National Academy of Sciences - PNAS 1993-01, Vol.90 (1), p.198-202 |
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| creator | Kleniewski, Jozef Donaldson, Virginia H. |
| description | Human umbilical vein endothelial cells (HUVECs) produce a property that impairs the generation of coagulant and amidolytic activity initiated when normal human plasma is exposed to glass. This inhibitory property blocks the adsorption of Hageman factor (factor XII) to glass, thereby preventing the activation of Hageman factor, but does not impair the coagulant or amidolytic activity of already activated Hageman factor (factor XIIa). This property in HUVEC lysates could be neutralized by a purified preparation of Hageman factor but not by purified prekallikrein or high molecular mass kininogen. A partially purified inhibitory fraction from cell lysates exhibited a single homogeneous band in SDS/PAGE of ≈22.5 kDa. Inhibitory activity was also found in concentrates of conditioned media from HUVECs, which also impaired the binding of Hageman factor to a surface; it may not be identical with that found in cell lysates. |
| doi_str_mv | 10.1073/pnas.90.1.198 |
| format | Article |
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This inhibitory property blocks the adsorption of Hageman factor (factor XII) to glass, thereby preventing the activation of Hageman factor, but does not impair the coagulant or amidolytic activity of already activated Hageman factor (factor XIIa). This property in HUVEC lysates could be neutralized by a purified preparation of Hageman factor but not by purified prekallikrein or high molecular mass kininogen. A partially purified inhibitory fraction from cell lysates exhibited a single homogeneous band in SDS/PAGE of ≈22.5 kDa. Inhibitory activity was also found in concentrates of conditioned media from HUVECs, which also impaired the binding of Hageman factor to a surface; it may not be identical with that found in cell lysates.</description><identifier>ISSN: 0027-8424</identifier><identifier>EISSN: 1091-6490</identifier><identifier>DOI: 10.1073/pnas.90.1.198</identifier><identifier>PMID: 8419923</identifier><identifier>CODEN: PNASA6</identifier><language>eng</language><publisher>Washington, DC: National Academy of Sciences of the United States of America</publisher><subject>activation ; Adsorption ; Biological and medical sciences ; Blood ; Blood coagulation. Blood cells ; Blood plasma ; Cells, Cultured ; Cellular biology ; Clotting ; Coagulants ; Culture Media ; Cultured cells ; Electrophoresis, Polyacrylamide Gel ; Endothelial cells ; Endothelium, Vascular - metabolism ; Factor XII - antagonists & inhibitors ; Factor XIIa - metabolism ; factors ; Fundamental and applied biological sciences. Psychology ; Gels ; General aspects, investigation methods, hemostasis, fibrinolysis ; Glass ; Hageman factor ; Humans ; inhibitory ; Kinetics ; Kininogens - metabolism ; Legends ; man ; Medical research ; Molecular and cellular biology ; Prekallikrein - metabolism ; umbilical vein ; Umbilical Veins</subject><ispartof>Proceedings of the National Academy of Sciences - PNAS, 1993-01, Vol.90 (1), p.198-202</ispartof><rights>Copyright 1993 The National Academy of Sciences of the United States of America</rights><rights>1993 INIST-CNRS</rights><rights>Copyright National Academy of Sciences Jan 1, 1993</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c539t-a3adae6ad3d8f3757a3b694462f27f12cc679812f53ae8c2fb9a48b776bc62853</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Uhttp://www.pnas.org/content/90/1.cover.gif</thumbnail><linktopdf>$$Uhttps://www.jstor.org/stable/pdf/2361017$$EPDF$$P50$$Gjstor$$H</linktopdf><linktohtml>$$Uhttps://www.jstor.org/stable/2361017$$EHTML$$P50$$Gjstor$$H</linktohtml><link.rule.ids>230,314,727,780,784,803,885,4024,27923,27924,27925,53791,53793,58017,58250</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4639563$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8419923$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Kleniewski, Jozef</creatorcontrib><creatorcontrib>Donaldson, Virginia H.</creatorcontrib><title>Endothelial Cells Produce a Substance that Inhibits Contact Activation of Coagulation by Blocking the Activation of Hageman Factor</title><title>Proceedings of the National Academy of Sciences - PNAS</title><addtitle>Proc Natl Acad Sci U S A</addtitle><description>Human umbilical vein endothelial cells (HUVECs) produce a property that impairs the generation of coagulant and amidolytic activity initiated when normal human plasma is exposed to glass. This inhibitory property blocks the adsorption of Hageman factor (factor XII) to glass, thereby preventing the activation of Hageman factor, but does not impair the coagulant or amidolytic activity of already activated Hageman factor (factor XIIa). This property in HUVEC lysates could be neutralized by a purified preparation of Hageman factor but not by purified prekallikrein or high molecular mass kininogen. A partially purified inhibitory fraction from cell lysates exhibited a single homogeneous band in SDS/PAGE of ≈22.5 kDa. Inhibitory activity was also found in concentrates of conditioned media from HUVECs, which also impaired the binding of Hageman factor to a surface; it may not be identical with that found in cell lysates.</description><subject>activation</subject><subject>Adsorption</subject><subject>Biological and medical sciences</subject><subject>Blood</subject><subject>Blood coagulation. Blood cells</subject><subject>Blood plasma</subject><subject>Cells, Cultured</subject><subject>Cellular biology</subject><subject>Clotting</subject><subject>Coagulants</subject><subject>Culture Media</subject><subject>Cultured cells</subject><subject>Electrophoresis, Polyacrylamide Gel</subject><subject>Endothelial cells</subject><subject>Endothelium, Vascular - metabolism</subject><subject>Factor XII - antagonists & inhibitors</subject><subject>Factor XIIa - metabolism</subject><subject>factors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gels</subject><subject>General aspects, investigation methods, hemostasis, fibrinolysis</subject><subject>Glass</subject><subject>Hageman factor</subject><subject>Humans</subject><subject>inhibitory</subject><subject>Kinetics</subject><subject>Kininogens - metabolism</subject><subject>Legends</subject><subject>man</subject><subject>Medical research</subject><subject>Molecular and cellular biology</subject><subject>Prekallikrein - metabolism</subject><subject>umbilical vein</subject><subject>Umbilical Veins</subject><issn>0027-8424</issn><issn>1091-6490</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkk1vEzEQhlcIVELhyA2kFQJuW_y1_pC4lKillSqBBJytWa83cXDsdO2t6JVfjqOEUHqAkz1-n3ltz0xVPcfoBCNB320CpBNVghOs5INqhpHCDWcKPaxmCBHRSEbY4-pJSiuEkGolOqqOJMNKETqrfp6FPual9Q58Pbfep_rzGPvJ2BrqL1OXMoSyz0vI9WVYus7lVM9jyGByfWqyu4HsYqjjUE5hMfld2N3WH3w0311YlFx7j7yAhV1DqM-LSRyfVo8G8Mk-26_H1bfzs6_zi-bq08fL-elVY1qqcgMUerAcetrLgYpWAO24YoyTgYgBE2O4UBKToaVgpSFDp4DJTgjeGU5kS4-r9zvfzdStbW9syCN4vRndGsZbHcHpv5XglnoRbzRrOREl_e0-fYzXk01Zr10ypWIQbJySFm3LJKP8vyDmjLQCswK-ugeu4jSGUgNNECaSKrV1a3aQGWNKox0OD8ZIbwdAbwdAqxLoMgCFf3n3lwd63_Giv97rkAz4YSz9demAMU5Vy7fYiz22df-t3rnlzT9kPUzeZ_sj_7FZpdLpA0goxwgL-gs05dt4</recordid><startdate>19930101</startdate><enddate>19930101</enddate><creator>Kleniewski, Jozef</creator><creator>Donaldson, Virginia H.</creator><general>National Academy of Sciences of the United States of America</general><general>National Acad Sciences</general><general>National Academy of Sciences</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QG</scope><scope>7QL</scope><scope>7QP</scope><scope>7QR</scope><scope>7SN</scope><scope>7SS</scope><scope>7T5</scope><scope>7TK</scope><scope>7TM</scope><scope>7TO</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>M7N</scope><scope>P64</scope><scope>RC3</scope><scope>M81</scope><scope>7X8</scope><scope>5PM</scope></search><sort><creationdate>19930101</creationdate><title>Endothelial Cells Produce a Substance that Inhibits Contact Activation of Coagulation by Blocking the Activation of Hageman Factor</title><author>Kleniewski, Jozef ; Donaldson, Virginia H.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c539t-a3adae6ad3d8f3757a3b694462f27f12cc679812f53ae8c2fb9a48b776bc62853</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>activation</topic><topic>Adsorption</topic><topic>Biological and medical sciences</topic><topic>Blood</topic><topic>Blood coagulation. Blood cells</topic><topic>Blood plasma</topic><topic>Cells, Cultured</topic><topic>Cellular biology</topic><topic>Clotting</topic><topic>Coagulants</topic><topic>Culture Media</topic><topic>Cultured cells</topic><topic>Electrophoresis, Polyacrylamide Gel</topic><topic>Endothelial cells</topic><topic>Endothelium, Vascular - metabolism</topic><topic>Factor XII - antagonists & inhibitors</topic><topic>Factor XIIa - metabolism</topic><topic>factors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gels</topic><topic>General aspects, investigation methods, hemostasis, fibrinolysis</topic><topic>Glass</topic><topic>Hageman factor</topic><topic>Humans</topic><topic>inhibitory</topic><topic>Kinetics</topic><topic>Kininogens - metabolism</topic><topic>Legends</topic><topic>man</topic><topic>Medical research</topic><topic>Molecular and cellular biology</topic><topic>Prekallikrein - metabolism</topic><topic>umbilical vein</topic><topic>Umbilical Veins</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Kleniewski, Jozef</creatorcontrib><creatorcontrib>Donaldson, Virginia H.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Animal Behavior Abstracts</collection><collection>Bacteriology Abstracts (Microbiology B)</collection><collection>Calcium & Calcified Tissue Abstracts</collection><collection>Chemoreception Abstracts</collection><collection>Ecology Abstracts</collection><collection>Entomology Abstracts (Full archive)</collection><collection>Immunology Abstracts</collection><collection>Neurosciences Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Oncogenes and Growth Factors Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>Engineering Research Database</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Algology Mycology and Protozoology Abstracts (Microbiology C)</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>Genetics Abstracts</collection><collection>Biochemistry Abstracts 3</collection><collection>MEDLINE - Academic</collection><collection>PubMed Central (Full Participant titles)</collection><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Kleniewski, Jozef</au><au>Donaldson, Virginia H.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Endothelial Cells Produce a Substance that Inhibits Contact Activation of Coagulation by Blocking the Activation of Hageman Factor</atitle><jtitle>Proceedings of the National Academy of Sciences - PNAS</jtitle><addtitle>Proc Natl Acad Sci U S A</addtitle><date>1993-01-01</date><risdate>1993</risdate><volume>90</volume><issue>1</issue><spage>198</spage><epage>202</epage><pages>198-202</pages><issn>0027-8424</issn><eissn>1091-6490</eissn><coden>PNASA6</coden><abstract>Human umbilical vein endothelial cells (HUVECs) produce a property that impairs the generation of coagulant and amidolytic activity initiated when normal human plasma is exposed to glass. This inhibitory property blocks the adsorption of Hageman factor (factor XII) to glass, thereby preventing the activation of Hageman factor, but does not impair the coagulant or amidolytic activity of already activated Hageman factor (factor XIIa). This property in HUVEC lysates could be neutralized by a purified preparation of Hageman factor but not by purified prekallikrein or high molecular mass kininogen. A partially purified inhibitory fraction from cell lysates exhibited a single homogeneous band in SDS/PAGE of ≈22.5 kDa. Inhibitory activity was also found in concentrates of conditioned media from HUVECs, which also impaired the binding of Hageman factor to a surface; it may not be identical with that found in cell lysates.</abstract><cop>Washington, DC</cop><pub>National Academy of Sciences of the United States of America</pub><pmid>8419923</pmid><doi>10.1073/pnas.90.1.198</doi><tpages>5</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | activation Adsorption Biological and medical sciences Blood Blood coagulation. Blood cells Blood plasma Cells, Cultured Cellular biology Clotting Coagulants Culture Media Cultured cells Electrophoresis, Polyacrylamide Gel Endothelial cells Endothelium, Vascular - metabolism Factor XII - antagonists & inhibitors Factor XIIa - metabolism factors Fundamental and applied biological sciences. Psychology Gels General aspects, investigation methods, hemostasis, fibrinolysis Glass Hageman factor Humans inhibitory Kinetics Kininogens - metabolism Legends man Medical research Molecular and cellular biology Prekallikrein - metabolism umbilical vein Umbilical Veins |
| title | Endothelial Cells Produce a Substance that Inhibits Contact Activation of Coagulation by Blocking the Activation of Hageman Factor |
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