Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma: Detection of CEA-mRNA in the three-field lymph nodes
Background and Objectives The aim of this study is to clarify the extent of lymphatic spread of cancer cells using a novel genetic test to examine patients with thoracic esophageal squamous cell carcinoma (ESCC). Methods A total of 35 patients who underwent an esophagectomy with three‐field lymph no...
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| Veröffentlicht in: | Journal of surgical oncology 2010-10, Vol.102 (5), p.509-515 |
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| creator | Imamura, Yu Hayashi, Naoko Sato, Nobutaka Kinoshita, Koichi Kurashige, Junji Saito, Seiya Hirashima, Kotaro Karashima, Ryuichi Hiyoshi, Yukiharu Nagai, Yohei Watanabe, Masayuki Baba, Hideo |
| description | Background and Objectives
The aim of this study is to clarify the extent of lymphatic spread of cancer cells using a novel genetic test to examine patients with thoracic esophageal squamous cell carcinoma (ESCC).
Methods
A total of 35 patients who underwent an esophagectomy with three‐field lymph node (LN) dissection were eligible. The regional LN stations were categorized into the cervical (C), recurrent nerve (RN), paraesophageal (PE), tracheo‐bronchial (TB), and perigastric (PG) nodes. Lymphatic spread was pathologically diagnosed with Hematoxylin‐Eosin (HE) and anti‐cytokeratin immunohistochemistry (IHC) staining, and CEA‐mRNA expression was examined using the transcription‐reverse transcription concerted (TRC) reaction.
Results
The rates of lymphatic spread with HE, IHC, and TRC were 7.2%, 10.1%, and 55.5%, respectively. The number of CEA‐mRNA(+) LN stations significantly correlated with tumor depth, LN metastasis diagnosed by HE, and vascular invasions. CEA‐mRNA expression was observed in 42.9%, 94.3%, 77.1%, 80.0%, and 82.9% of C, RN, TB, PE, and PG nodes, respectively.
Conclusions
The high frequency of CEA‐mRNA expression suggests that systemic therapy is necessary in addition to esophagectomy with adequate LN dissection. Conversely, a relatively low frequency of CEA‐mRNA expression in the C node does not support the routine dissection of the LNs in this area. J. Surg. Oncol. 2010;102:509–515. © 2010 Wiley‐Liss, Inc. |
| doi_str_mv | 10.1002/jso.21621 |
| format | Article |
| fullrecord | <record><control><sourceid>proquest_cross</sourceid><recordid>TN_cdi_proquest_miscellaneous_755400967</recordid><sourceformat>XML</sourceformat><sourcesystem>PC</sourcesystem><sourcerecordid>755400967</sourcerecordid><originalsourceid>FETCH-LOGICAL-c3621-50e257bb313d6f7a1c1253aa0ca51100e2b74688a8ff8b5f152b78e7fac265873</originalsourceid><addsrcrecordid>eNp1UcFu1DAUtBCILi0HfgD5hjiktZ04drittksBrVqpUPVoOc4L65LEqe2l3Y_hX3GatrcerCf5zYzezCD0gZJjSgg7uQnumNGS0VdoQUlVZhWp5Gu0SDuWFaIiB-hdCDeEkKoqi7fogBEpWMWLBfq3vo8wBPsXcLfvx62O1uAwetANdi02ejDgsYGuC9gOeEx7GGLAdzZucdw6r00iQHCJ-ht0h8PtTvduFx44ie-NHVyvv-BTiGCidcOku1ovs_7yfDlpxi2k5wGy1kLXzHfgwTUQjtCbVncB3j_OQ3T1df1r9S3bXJx9Xy03mcmT64wTYFzUdU7zpmyFpoYynmtNjOY0JQSsFkUppZZtK2veUp4-JIhWG1ZyKfJD9GnWHb273UGIqrdhMqAHSF6U4LxI4ZUT8vOMNN6F4KFVo7e99ntFiZrKUKkM9VBGwn58VN3VPTTPyKf0E-BkBtzZDvYvK6kfPy-eJLOZYUOE-2eG9n9UOk5wdX1-pjZ5QalYXSqZ_wefGaRc</addsrcrecordid><sourcetype>Aggregation Database</sourcetype><iscdi>true</iscdi><recordtype>article</recordtype><pqid>755400967</pqid></control><display><type>article</type><title>Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma: Detection of CEA-mRNA in the three-field lymph nodes</title><source>Wiley Online Library - AutoHoldings Journals</source><source>MEDLINE</source><creator>Imamura, Yu ; Hayashi, Naoko ; Sato, Nobutaka ; Kinoshita, Koichi ; Kurashige, Junji ; Saito, Seiya ; Hirashima, Kotaro ; Karashima, Ryuichi ; Hiyoshi, Yukiharu ; Nagai, Yohei ; Watanabe, Masayuki ; Baba, Hideo</creator><creatorcontrib>Imamura, Yu ; Hayashi, Naoko ; Sato, Nobutaka ; Kinoshita, Koichi ; Kurashige, Junji ; Saito, Seiya ; Hirashima, Kotaro ; Karashima, Ryuichi ; Hiyoshi, Yukiharu ; Nagai, Yohei ; Watanabe, Masayuki ; Baba, Hideo</creatorcontrib><description>Background and Objectives
The aim of this study is to clarify the extent of lymphatic spread of cancer cells using a novel genetic test to examine patients with thoracic esophageal squamous cell carcinoma (ESCC).
Methods
A total of 35 patients who underwent an esophagectomy with three‐field lymph node (LN) dissection were eligible. The regional LN stations were categorized into the cervical (C), recurrent nerve (RN), paraesophageal (PE), tracheo‐bronchial (TB), and perigastric (PG) nodes. Lymphatic spread was pathologically diagnosed with Hematoxylin‐Eosin (HE) and anti‐cytokeratin immunohistochemistry (IHC) staining, and CEA‐mRNA expression was examined using the transcription‐reverse transcription concerted (TRC) reaction.
Results
The rates of lymphatic spread with HE, IHC, and TRC were 7.2%, 10.1%, and 55.5%, respectively. The number of CEA‐mRNA(+) LN stations significantly correlated with tumor depth, LN metastasis diagnosed by HE, and vascular invasions. CEA‐mRNA expression was observed in 42.9%, 94.3%, 77.1%, 80.0%, and 82.9% of C, RN, TB, PE, and PG nodes, respectively.
Conclusions
The high frequency of CEA‐mRNA expression suggests that systemic therapy is necessary in addition to esophagectomy with adequate LN dissection. Conversely, a relatively low frequency of CEA‐mRNA expression in the C node does not support the routine dissection of the LNs in this area. J. Surg. Oncol. 2010;102:509–515. © 2010 Wiley‐Liss, Inc.</description><identifier>ISSN: 0022-4790</identifier><identifier>EISSN: 1096-9098</identifier><identifier>DOI: 10.1002/jso.21621</identifier><identifier>PMID: 20872954</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Aged ; Carcinoembryonic Antigen - metabolism ; Carcinoma, Squamous Cell - secondary ; Carcinoma, Squamous Cell - surgery ; CEA-mRNA ; esophageal cancer ; Esophageal Neoplasms - pathology ; Esophageal Neoplasms - surgery ; Esophagectomy ; Female ; Humans ; lymph node dissection ; Lymph Node Excision ; lymph node micrometastasis ; Lymph Nodes - metabolism ; Lymph Nodes - pathology ; Lymph Nodes - surgery ; Lymphatic Metastasis ; Male ; Middle Aged ; neoadjuvant therapy ; Prospective Studies ; RNA, Messenger - metabolism</subject><ispartof>Journal of surgical oncology, 2010-10, Vol.102 (5), p.509-515</ispartof><rights>Copyright © 2010 Wiley‐Liss, Inc.</rights><rights>J. Surg. Oncol. 2010;102:509-515. © 2010 Wiley-Liss, Inc.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3621-50e257bb313d6f7a1c1253aa0ca51100e2b74688a8ff8b5f152b78e7fac265873</citedby><cites>FETCH-LOGICAL-c3621-50e257bb313d6f7a1c1253aa0ca51100e2b74688a8ff8b5f152b78e7fac265873</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fjso.21621$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fjso.21621$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,778,782,1414,27907,27908,45557,45558</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20872954$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Imamura, Yu</creatorcontrib><creatorcontrib>Hayashi, Naoko</creatorcontrib><creatorcontrib>Sato, Nobutaka</creatorcontrib><creatorcontrib>Kinoshita, Koichi</creatorcontrib><creatorcontrib>Kurashige, Junji</creatorcontrib><creatorcontrib>Saito, Seiya</creatorcontrib><creatorcontrib>Hirashima, Kotaro</creatorcontrib><creatorcontrib>Karashima, Ryuichi</creatorcontrib><creatorcontrib>Hiyoshi, Yukiharu</creatorcontrib><creatorcontrib>Nagai, Yohei</creatorcontrib><creatorcontrib>Watanabe, Masayuki</creatorcontrib><creatorcontrib>Baba, Hideo</creatorcontrib><title>Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma: Detection of CEA-mRNA in the three-field lymph nodes</title><title>Journal of surgical oncology</title><addtitle>J. Surg. Oncol</addtitle><description>Background and Objectives
The aim of this study is to clarify the extent of lymphatic spread of cancer cells using a novel genetic test to examine patients with thoracic esophageal squamous cell carcinoma (ESCC).
Methods
A total of 35 patients who underwent an esophagectomy with three‐field lymph node (LN) dissection were eligible. The regional LN stations were categorized into the cervical (C), recurrent nerve (RN), paraesophageal (PE), tracheo‐bronchial (TB), and perigastric (PG) nodes. Lymphatic spread was pathologically diagnosed with Hematoxylin‐Eosin (HE) and anti‐cytokeratin immunohistochemistry (IHC) staining, and CEA‐mRNA expression was examined using the transcription‐reverse transcription concerted (TRC) reaction.
Results
The rates of lymphatic spread with HE, IHC, and TRC were 7.2%, 10.1%, and 55.5%, respectively. The number of CEA‐mRNA(+) LN stations significantly correlated with tumor depth, LN metastasis diagnosed by HE, and vascular invasions. CEA‐mRNA expression was observed in 42.9%, 94.3%, 77.1%, 80.0%, and 82.9% of C, RN, TB, PE, and PG nodes, respectively.
Conclusions
The high frequency of CEA‐mRNA expression suggests that systemic therapy is necessary in addition to esophagectomy with adequate LN dissection. Conversely, a relatively low frequency of CEA‐mRNA expression in the C node does not support the routine dissection of the LNs in this area. J. Surg. Oncol. 2010;102:509–515. © 2010 Wiley‐Liss, Inc.</description><subject>Aged</subject><subject>Carcinoembryonic Antigen - metabolism</subject><subject>Carcinoma, Squamous Cell - secondary</subject><subject>Carcinoma, Squamous Cell - surgery</subject><subject>CEA-mRNA</subject><subject>esophageal cancer</subject><subject>Esophageal Neoplasms - pathology</subject><subject>Esophageal Neoplasms - surgery</subject><subject>Esophagectomy</subject><subject>Female</subject><subject>Humans</subject><subject>lymph node dissection</subject><subject>Lymph Node Excision</subject><subject>lymph node micrometastasis</subject><subject>Lymph Nodes - metabolism</subject><subject>Lymph Nodes - pathology</subject><subject>Lymph Nodes - surgery</subject><subject>Lymphatic Metastasis</subject><subject>Male</subject><subject>Middle Aged</subject><subject>neoadjuvant therapy</subject><subject>Prospective Studies</subject><subject>RNA, Messenger - metabolism</subject><issn>0022-4790</issn><issn>1096-9098</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1UcFu1DAUtBCILi0HfgD5hjiktZ04drittksBrVqpUPVoOc4L65LEqe2l3Y_hX3GatrcerCf5zYzezCD0gZJjSgg7uQnumNGS0VdoQUlVZhWp5Gu0SDuWFaIiB-hdCDeEkKoqi7fogBEpWMWLBfq3vo8wBPsXcLfvx62O1uAwetANdi02ejDgsYGuC9gOeEx7GGLAdzZucdw6r00iQHCJ-ht0h8PtTvduFx44ie-NHVyvv-BTiGCidcOku1ovs_7yfDlpxi2k5wGy1kLXzHfgwTUQjtCbVncB3j_OQ3T1df1r9S3bXJx9Xy03mcmT64wTYFzUdU7zpmyFpoYynmtNjOY0JQSsFkUppZZtK2veUp4-JIhWG1ZyKfJD9GnWHb273UGIqrdhMqAHSF6U4LxI4ZUT8vOMNN6F4KFVo7e99ntFiZrKUKkM9VBGwn58VN3VPTTPyKf0E-BkBtzZDvYvK6kfPy-eJLOZYUOE-2eG9n9UOk5wdX1-pjZ5QalYXSqZ_wefGaRc</recordid><startdate>20101001</startdate><enddate>20101001</enddate><creator>Imamura, Yu</creator><creator>Hayashi, Naoko</creator><creator>Sato, Nobutaka</creator><creator>Kinoshita, Koichi</creator><creator>Kurashige, Junji</creator><creator>Saito, Seiya</creator><creator>Hirashima, Kotaro</creator><creator>Karashima, Ryuichi</creator><creator>Hiyoshi, Yukiharu</creator><creator>Nagai, Yohei</creator><creator>Watanabe, Masayuki</creator><creator>Baba, Hideo</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><scope>BSCLL</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101001</creationdate><title>Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma: Detection of CEA-mRNA in the three-field lymph nodes</title><author>Imamura, Yu ; Hayashi, Naoko ; Sato, Nobutaka ; Kinoshita, Koichi ; Kurashige, Junji ; Saito, Seiya ; Hirashima, Kotaro ; Karashima, Ryuichi ; Hiyoshi, Yukiharu ; Nagai, Yohei ; Watanabe, Masayuki ; Baba, Hideo</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3621-50e257bb313d6f7a1c1253aa0ca51100e2b74688a8ff8b5f152b78e7fac265873</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aged</topic><topic>Carcinoembryonic Antigen - metabolism</topic><topic>Carcinoma, Squamous Cell - secondary</topic><topic>Carcinoma, Squamous Cell - surgery</topic><topic>CEA-mRNA</topic><topic>esophageal cancer</topic><topic>Esophageal Neoplasms - pathology</topic><topic>Esophageal Neoplasms - surgery</topic><topic>Esophagectomy</topic><topic>Female</topic><topic>Humans</topic><topic>lymph node dissection</topic><topic>Lymph Node Excision</topic><topic>lymph node micrometastasis</topic><topic>Lymph Nodes - metabolism</topic><topic>Lymph Nodes - pathology</topic><topic>Lymph Nodes - surgery</topic><topic>Lymphatic Metastasis</topic><topic>Male</topic><topic>Middle Aged</topic><topic>neoadjuvant therapy</topic><topic>Prospective Studies</topic><topic>RNA, Messenger - metabolism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Imamura, Yu</creatorcontrib><creatorcontrib>Hayashi, Naoko</creatorcontrib><creatorcontrib>Sato, Nobutaka</creatorcontrib><creatorcontrib>Kinoshita, Koichi</creatorcontrib><creatorcontrib>Kurashige, Junji</creatorcontrib><creatorcontrib>Saito, Seiya</creatorcontrib><creatorcontrib>Hirashima, Kotaro</creatorcontrib><creatorcontrib>Karashima, Ryuichi</creatorcontrib><creatorcontrib>Hiyoshi, Yukiharu</creatorcontrib><creatorcontrib>Nagai, Yohei</creatorcontrib><creatorcontrib>Watanabe, Masayuki</creatorcontrib><creatorcontrib>Baba, Hideo</creatorcontrib><collection>Istex</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of surgical oncology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Imamura, Yu</au><au>Hayashi, Naoko</au><au>Sato, Nobutaka</au><au>Kinoshita, Koichi</au><au>Kurashige, Junji</au><au>Saito, Seiya</au><au>Hirashima, Kotaro</au><au>Karashima, Ryuichi</au><au>Hiyoshi, Yukiharu</au><au>Nagai, Yohei</au><au>Watanabe, Masayuki</au><au>Baba, Hideo</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma: Detection of CEA-mRNA in the three-field lymph nodes</atitle><jtitle>Journal of surgical oncology</jtitle><addtitle>J. Surg. Oncol</addtitle><date>2010-10-01</date><risdate>2010</risdate><volume>102</volume><issue>5</issue><spage>509</spage><epage>515</epage><pages>509-515</pages><issn>0022-4790</issn><eissn>1096-9098</eissn><abstract>Background and Objectives
The aim of this study is to clarify the extent of lymphatic spread of cancer cells using a novel genetic test to examine patients with thoracic esophageal squamous cell carcinoma (ESCC).
Methods
A total of 35 patients who underwent an esophagectomy with three‐field lymph node (LN) dissection were eligible. The regional LN stations were categorized into the cervical (C), recurrent nerve (RN), paraesophageal (PE), tracheo‐bronchial (TB), and perigastric (PG) nodes. Lymphatic spread was pathologically diagnosed with Hematoxylin‐Eosin (HE) and anti‐cytokeratin immunohistochemistry (IHC) staining, and CEA‐mRNA expression was examined using the transcription‐reverse transcription concerted (TRC) reaction.
Results
The rates of lymphatic spread with HE, IHC, and TRC were 7.2%, 10.1%, and 55.5%, respectively. The number of CEA‐mRNA(+) LN stations significantly correlated with tumor depth, LN metastasis diagnosed by HE, and vascular invasions. CEA‐mRNA expression was observed in 42.9%, 94.3%, 77.1%, 80.0%, and 82.9% of C, RN, TB, PE, and PG nodes, respectively.
Conclusions
The high frequency of CEA‐mRNA expression suggests that systemic therapy is necessary in addition to esophagectomy with adequate LN dissection. Conversely, a relatively low frequency of CEA‐mRNA expression in the C node does not support the routine dissection of the LNs in this area. J. Surg. Oncol. 2010;102:509–515. © 2010 Wiley‐Liss, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>20872954</pmid><doi>10.1002/jso.21621</doi><tpages>7</tpages></addata></record> |
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| subjects | Aged Carcinoembryonic Antigen - metabolism Carcinoma, Squamous Cell - secondary Carcinoma, Squamous Cell - surgery CEA-mRNA esophageal cancer Esophageal Neoplasms - pathology Esophageal Neoplasms - surgery Esophagectomy Female Humans lymph node dissection Lymph Node Excision lymph node micrometastasis Lymph Nodes - metabolism Lymph Nodes - pathology Lymph Nodes - surgery Lymphatic Metastasis Male Middle Aged neoadjuvant therapy Prospective Studies RNA, Messenger - metabolism |
| title | Extensive lymphatic spread of cancer cells in patients with thoracic esophageal squamous cell carcinoma: Detection of CEA-mRNA in the three-field lymph nodes |
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