Colorimetric genotyping of single nucleotide polymorphism based on selective aggregation of unmodified gold nanoparticles
We have developed a colorimetric method for genotyping of single nucleotide polymorphism (rs2131877) in 15 human DNA samples using selective aggregation of unmodified gold nanoparticles. First, we designed two different types of oligonucleotide probes with either thymine or cytosine at the end that...
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| Veröffentlicht in: | Biosensors & bioelectronics 2010-10, Vol.26 (2), p.730-735 |
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| creator | Lee, Hosub Joo, Sang-Woo Lee, So Yeong Lee, Chang-Ha Yoon, Kyong-Ah Lee, Kangtaek |
| description | We have developed a colorimetric method for genotyping of single nucleotide polymorphism (rs2131877) in 15 human DNA samples using selective aggregation of unmodified gold nanoparticles. First, we designed two different types of oligonucleotide probes with either thymine or cytosine at the end that are perfect complementary to the target allele sequence. After hybridization of the probe and target DNAs, the double-stranded DNA was added to the unmodified gold nanoparticle suspensions. By adjusting the salt concentration, we could induce aggregation of gold nanoparticles exclusively for the samples with the perfectly matched double-stranded DNAs, which resulted in a distinct color change of the suspension. This enabled us to discern samples with three different genotypes of the target sequences by naked eye: (i) the genotype with only T (thymine) alleles; (ii) that with only C (cytosine) alleles; (iii) that with both T and C alleles. We also confirmed these results by an independent direct sequencing method. These results suggest that the selective aggregation of unmodified gold nanoparticles can successfully be used to discern genotypes of single nucleotide polymorphisms. |
| doi_str_mv | 10.1016/j.bios.2010.06.050 |
| format | Article |
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First, we designed two different types of oligonucleotide probes with either thymine or cytosine at the end that are perfect complementary to the target allele sequence. After hybridization of the probe and target DNAs, the double-stranded DNA was added to the unmodified gold nanoparticle suspensions. By adjusting the salt concentration, we could induce aggregation of gold nanoparticles exclusively for the samples with the perfectly matched double-stranded DNAs, which resulted in a distinct color change of the suspension. This enabled us to discern samples with three different genotypes of the target sequences by naked eye: (i) the genotype with only T (thymine) alleles; (ii) that with only C (cytosine) alleles; (iii) that with both T and C alleles. We also confirmed these results by an independent direct sequencing method. These results suggest that the selective aggregation of unmodified gold nanoparticles can successfully be used to discern genotypes of single nucleotide polymorphisms.</description><identifier>ISSN: 0956-5663</identifier><identifier>EISSN: 1873-4235</identifier><identifier>DOI: 10.1016/j.bios.2010.06.050</identifier><identifier>PMID: 20674325</identifier><language>eng</language><publisher>Kidlington: Elsevier B.V</publisher><subject>Aggregation ; Biological and medical sciences ; Biosensing Techniques - instrumentation ; Biotechnology ; Colorimetric sensor ; Colorimetry - instrumentation ; DNA ; DNA Mutational Analysis - instrumentation ; Equipment Design ; Equipment Failure Analysis ; Fundamental and applied biological sciences. Psychology ; Genotype ; Genotypes ; Gold - chemistry ; Gold nanoparticles ; Humans ; Nanoparticles - chemistry ; Polymorphism, Single Nucleotide - genetics ; Sequence Analysis, DNA - instrumentation ; Single nucleotide polymorphism</subject><ispartof>Biosensors & bioelectronics, 2010-10, Vol.26 (2), p.730-735</ispartof><rights>2010 Elsevier B.V.</rights><rights>2015 INIST-CNRS</rights><rights>Copyright © 2010 Elsevier B.V. All rights reserved.</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c451t-e36acddc768571ea5ca220a0e58744b32320e3f75c2e2667a78aab2efc343edd3</citedby><cites>FETCH-LOGICAL-c451t-e36acddc768571ea5ca220a0e58744b32320e3f75c2e2667a78aab2efc343edd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/j.bios.2010.06.050$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>315,781,785,3551,27929,27930,46000</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23360670$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20674325$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Lee, Hosub</creatorcontrib><creatorcontrib>Joo, Sang-Woo</creatorcontrib><creatorcontrib>Lee, So Yeong</creatorcontrib><creatorcontrib>Lee, Chang-Ha</creatorcontrib><creatorcontrib>Yoon, Kyong-Ah</creatorcontrib><creatorcontrib>Lee, Kangtaek</creatorcontrib><title>Colorimetric genotyping of single nucleotide polymorphism based on selective aggregation of unmodified gold nanoparticles</title><title>Biosensors & bioelectronics</title><addtitle>Biosens Bioelectron</addtitle><description>We have developed a colorimetric method for genotyping of single nucleotide polymorphism (rs2131877) in 15 human DNA samples using selective aggregation of unmodified gold nanoparticles. First, we designed two different types of oligonucleotide probes with either thymine or cytosine at the end that are perfect complementary to the target allele sequence. After hybridization of the probe and target DNAs, the double-stranded DNA was added to the unmodified gold nanoparticle suspensions. By adjusting the salt concentration, we could induce aggregation of gold nanoparticles exclusively for the samples with the perfectly matched double-stranded DNAs, which resulted in a distinct color change of the suspension. This enabled us to discern samples with three different genotypes of the target sequences by naked eye: (i) the genotype with only T (thymine) alleles; (ii) that with only C (cytosine) alleles; (iii) that with both T and C alleles. We also confirmed these results by an independent direct sequencing method. These results suggest that the selective aggregation of unmodified gold nanoparticles can successfully be used to discern genotypes of single nucleotide polymorphisms.</description><subject>Aggregation</subject><subject>Biological and medical sciences</subject><subject>Biosensing Techniques - instrumentation</subject><subject>Biotechnology</subject><subject>Colorimetric sensor</subject><subject>Colorimetry - instrumentation</subject><subject>DNA</subject><subject>DNA Mutational Analysis - instrumentation</subject><subject>Equipment Design</subject><subject>Equipment Failure Analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Genotype</subject><subject>Genotypes</subject><subject>Gold - chemistry</subject><subject>Gold nanoparticles</subject><subject>Humans</subject><subject>Nanoparticles - chemistry</subject><subject>Polymorphism, Single Nucleotide - genetics</subject><subject>Sequence Analysis, DNA - instrumentation</subject><subject>Single nucleotide polymorphism</subject><issn>0956-5663</issn><issn>1873-4235</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMGKFDEQQIMo7uzqD3iQXMRTj-mkk8yAFxl0FRa86DlUJ9VthnTSJt0L8_ebYUa9eSooXj2KR8iblm1b1qoPx23vU9lyVhdMbZlkz8im3WnRdFzI52TD9lI1UilxQ25LOTLGdLtnL8kNZ0p3gssNOR1SSNlPuGRv6YgxLafZx5GmgZY6A9K42oBp8Q7pnMJpSnn-5ctEeyjoaIq0YEC7-EekMI4ZR1h83VbBGqfk_OArNqbgaISYZsiLr8LyirwYIBR8fZ135OeXzz8OX5uH7_ffDp8eGtvJdmlQKLDOWa12UrcI0gLnDBjKne66XnDBGYpBS8uRK6VB7wB6joMVnUDnxB15f_HOOf1esSxm8sViCBAxrcVoKcV-r_ZdJfmFtDmVknEwcy0D-WRaZs7FzdGci5tzccOUqcXr0durfu0ndH9P_iSuwLsrAMVCGDJE68s_TghV0bPo44XDGuPRYzbFeowWnc81r3HJ_--PJ0Vtop8</recordid><startdate>20101015</startdate><enddate>20101015</enddate><creator>Lee, Hosub</creator><creator>Joo, Sang-Woo</creator><creator>Lee, So Yeong</creator><creator>Lee, Chang-Ha</creator><creator>Yoon, Kyong-Ah</creator><creator>Lee, Kangtaek</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>20101015</creationdate><title>Colorimetric genotyping of single nucleotide polymorphism based on selective aggregation of unmodified gold nanoparticles</title><author>Lee, Hosub ; Joo, Sang-Woo ; Lee, So Yeong ; Lee, Chang-Ha ; Yoon, Kyong-Ah ; Lee, Kangtaek</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c451t-e36acddc768571ea5ca220a0e58744b32320e3f75c2e2667a78aab2efc343edd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Aggregation</topic><topic>Biological and medical sciences</topic><topic>Biosensing Techniques - instrumentation</topic><topic>Biotechnology</topic><topic>Colorimetric sensor</topic><topic>Colorimetry - instrumentation</topic><topic>DNA</topic><topic>DNA Mutational Analysis - instrumentation</topic><topic>Equipment Design</topic><topic>Equipment Failure Analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Genotype</topic><topic>Genotypes</topic><topic>Gold - chemistry</topic><topic>Gold nanoparticles</topic><topic>Humans</topic><topic>Nanoparticles - chemistry</topic><topic>Polymorphism, Single Nucleotide - genetics</topic><topic>Sequence Analysis, DNA - instrumentation</topic><topic>Single nucleotide polymorphism</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Lee, Hosub</creatorcontrib><creatorcontrib>Joo, Sang-Woo</creatorcontrib><creatorcontrib>Lee, So Yeong</creatorcontrib><creatorcontrib>Lee, Chang-Ha</creatorcontrib><creatorcontrib>Yoon, Kyong-Ah</creatorcontrib><creatorcontrib>Lee, Kangtaek</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Biosensors & bioelectronics</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Lee, Hosub</au><au>Joo, Sang-Woo</au><au>Lee, So Yeong</au><au>Lee, Chang-Ha</au><au>Yoon, Kyong-Ah</au><au>Lee, Kangtaek</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Colorimetric genotyping of single nucleotide polymorphism based on selective aggregation of unmodified gold nanoparticles</atitle><jtitle>Biosensors & bioelectronics</jtitle><addtitle>Biosens Bioelectron</addtitle><date>2010-10-15</date><risdate>2010</risdate><volume>26</volume><issue>2</issue><spage>730</spage><epage>735</epage><pages>730-735</pages><issn>0956-5663</issn><eissn>1873-4235</eissn><abstract>We have developed a colorimetric method for genotyping of single nucleotide polymorphism (rs2131877) in 15 human DNA samples using selective aggregation of unmodified gold nanoparticles. First, we designed two different types of oligonucleotide probes with either thymine or cytosine at the end that are perfect complementary to the target allele sequence. After hybridization of the probe and target DNAs, the double-stranded DNA was added to the unmodified gold nanoparticle suspensions. By adjusting the salt concentration, we could induce aggregation of gold nanoparticles exclusively for the samples with the perfectly matched double-stranded DNAs, which resulted in a distinct color change of the suspension. This enabled us to discern samples with three different genotypes of the target sequences by naked eye: (i) the genotype with only T (thymine) alleles; (ii) that with only C (cytosine) alleles; (iii) that with both T and C alleles. We also confirmed these results by an independent direct sequencing method. These results suggest that the selective aggregation of unmodified gold nanoparticles can successfully be used to discern genotypes of single nucleotide polymorphisms.</abstract><cop>Kidlington</cop><pub>Elsevier B.V</pub><pmid>20674325</pmid><doi>10.1016/j.bios.2010.06.050</doi><tpages>6</tpages></addata></record> |
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| source | MEDLINE; ScienceDirect Journals (5 years ago - present) |
| subjects | Aggregation Biological and medical sciences Biosensing Techniques - instrumentation Biotechnology Colorimetric sensor Colorimetry - instrumentation DNA DNA Mutational Analysis - instrumentation Equipment Design Equipment Failure Analysis Fundamental and applied biological sciences. Psychology Genotype Genotypes Gold - chemistry Gold nanoparticles Humans Nanoparticles - chemistry Polymorphism, Single Nucleotide - genetics Sequence Analysis, DNA - instrumentation Single nucleotide polymorphism |
| title | Colorimetric genotyping of single nucleotide polymorphism based on selective aggregation of unmodified gold nanoparticles |
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