Allelic variations clustered in the antigen binding sites of HLA-Bw62 molecules
HLA-Bw62 is a serologically defined class I antigen specificity, but we show that it represents a family of five distinct alleles in this study. Five variants of HLA-Bw62 antigens were identified by isoelectric focusing, and sequencing studies revealed that these are a family of closely related alle...
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| Veröffentlicht in: | Immunogenetics (New York) 1993, Vol.37 (2), p.108-113 |
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| creator | YOON CHOO, S LI-AN FAN HANSEN, J. A |
| description | HLA-Bw62 is a serologically defined class I antigen specificity, but we show that it represents a family of five distinct alleles in this study. Five variants of HLA-Bw62 antigens were identified by isoelectric focusing, and sequencing studies revealed that these are a family of closely related alleles differing from one another by one to six amino acid substitutions at eight positions: 63 in the alpha 1 domain and 94, 95, 97, 99, 113, 152, and 156 in the alpha 2 domain. These substitutions are located in the two alpha-helices and two adjacent beta-strands, and the side chains of most amino acids face into the antigen binding groove. Functional assays using an in vitro generated Epstein-Barr virus (EBV)-specific Bw62-restricted cytotoxic T lymphocyte clone indicated that the minimal structural variations located in the antigen binding sites of the HLA-Bw62 variant molecules could affect the presentation of the nominal EBV antigen. This study revealed that the HLA-Bw62 antigen family consists of at least five closely related alleles, and further demonstrated that these alleles with minimal structural variations might play distinct functional roles in regard to antigen presentation. |
| doi_str_mv | 10.1007/BF00216833 |
| format | Article |
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Functional assays using an in vitro generated Epstein-Barr virus (EBV)-specific Bw62-restricted cytotoxic T lymphocyte clone indicated that the minimal structural variations located in the antigen binding sites of the HLA-Bw62 variant molecules could affect the presentation of the nominal EBV antigen. 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Psychology ; Fundamental immunology ; genetic variance ; histocompatibility antigen HLA ; Histocompatibility antigens (hla, h-2 and other systems) ; HLA-B Antigens - chemistry ; HLA-B Antigens - genetics ; HLA-B Antigens - immunology ; HLA-B15 Antigen ; Humans ; Isoelectric Focusing ; man ; Molecular immunology ; Molecular Sequence Data ; Phenotype ; Protein Structure, Tertiary ; sites ; T-Lymphocytes, Cytotoxic - immunology</subject><ispartof>Immunogenetics (New York), 1993, Vol.37 (2), p.108-113</ispartof><rights>1993 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c342t-7b966d8ff5188abf28aeae2aeba041c64e2542a2417e80d87d3878007ebdfd463</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,777,781,4010,27904,27905,27906</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4478120$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8423049$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>YOON CHOO, S</creatorcontrib><creatorcontrib>LI-AN FAN</creatorcontrib><creatorcontrib>HANSEN, J. A</creatorcontrib><title>Allelic variations clustered in the antigen binding sites of HLA-Bw62 molecules</title><title>Immunogenetics (New York)</title><addtitle>Immunogenetics</addtitle><description>HLA-Bw62 is a serologically defined class I antigen specificity, but we show that it represents a family of five distinct alleles in this study. Five variants of HLA-Bw62 antigens were identified by isoelectric focusing, and sequencing studies revealed that these are a family of closely related alleles differing from one another by one to six amino acid substitutions at eight positions: 63 in the alpha 1 domain and 94, 95, 97, 99, 113, 152, and 156 in the alpha 2 domain. These substitutions are located in the two alpha-helices and two adjacent beta-strands, and the side chains of most amino acids face into the antigen binding groove. Functional assays using an in vitro generated Epstein-Barr virus (EBV)-specific Bw62-restricted cytotoxic T lymphocyte clone indicated that the minimal structural variations located in the antigen binding sites of the HLA-Bw62 variant molecules could affect the presentation of the nominal EBV antigen. This study revealed that the HLA-Bw62 antigen family consists of at least five closely related alleles, and further demonstrated that these alleles with minimal structural variations might play distinct functional roles in regard to antigen presentation.</description><subject>Alleles</subject><subject>Amino Acid Sequence</subject><subject>Antigens</subject><subject>Base Sequence</subject><subject>binding</subject><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Bw62 determinant</subject><subject>Cell Line</subject><subject>DNA</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Fundamental immunology</subject><subject>genetic variance</subject><subject>histocompatibility antigen HLA</subject><subject>Histocompatibility antigens (hla, h-2 and other systems)</subject><subject>HLA-B Antigens - chemistry</subject><subject>HLA-B Antigens - genetics</subject><subject>HLA-B Antigens - immunology</subject><subject>HLA-B15 Antigen</subject><subject>Humans</subject><subject>Isoelectric Focusing</subject><subject>man</subject><subject>Molecular immunology</subject><subject>Molecular Sequence Data</subject><subject>Phenotype</subject><subject>Protein Structure, Tertiary</subject><subject>sites</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><issn>0093-7711</issn><issn>1432-1211</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqF0MFLwzAUBvAgypzTi3chB_EgVPOSNEmP23BOGOyi55KmrzOStdq0iv-9lY159PQO348P3kfIJbA7YEzfzxaMcVBGiCMyBil4AhzgmIwZy0SiNcApOYvxjTFIM65GZGQkF0xmY7KehoDBO_ppW28739SRutDHDlssqa9p94rU1p3fYE0LX5e-3tDoO4y0qehyNU1mX4rTbRPQ9QHjOTmpbIh4sb8T8rJ4eJ4vk9X68Wk-XSVOSN4lusiUKk1VpWCMLSpuLFrkFgvLJDglkaeSWy5Bo2Gl0aUw2gy_YlFWpVRiQm52ve9t89Fj7PKtjw5DsDU2fcx1mgojuP4XgpKZgiwb4O0OuraJscUqf2_91rbfObD8d-b8b-YBX-1b-2KL5YHudx3y631uo7Oham3tfDwwKbUBzsQPr_KCyw</recordid><startdate>1993</startdate><enddate>1993</enddate><creator>YOON CHOO, S</creator><creator>LI-AN FAN</creator><creator>HANSEN, J. 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A</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c342t-7b966d8ff5188abf28aeae2aeba041c64e2542a2417e80d87d3878007ebdfd463</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Alleles</topic><topic>Amino Acid Sequence</topic><topic>Antigens</topic><topic>Base Sequence</topic><topic>binding</topic><topic>Binding Sites</topic><topic>Biological and medical sciences</topic><topic>Bw62 determinant</topic><topic>Cell Line</topic><topic>DNA</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Fundamental immunology</topic><topic>genetic variance</topic><topic>histocompatibility antigen HLA</topic><topic>Histocompatibility antigens (hla, h-2 and other systems)</topic><topic>HLA-B Antigens - chemistry</topic><topic>HLA-B Antigens - genetics</topic><topic>HLA-B Antigens - immunology</topic><topic>HLA-B15 Antigen</topic><topic>Humans</topic><topic>Isoelectric Focusing</topic><topic>man</topic><topic>Molecular immunology</topic><topic>Molecular Sequence Data</topic><topic>Phenotype</topic><topic>Protein Structure, Tertiary</topic><topic>sites</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>YOON CHOO, S</creatorcontrib><creatorcontrib>LI-AN FAN</creatorcontrib><creatorcontrib>HANSEN, J. 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| ispartof | Immunogenetics (New York), 1993, Vol.37 (2), p.108-113 |
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| source | MEDLINE; SpringerLink Journals |
| subjects | Alleles Amino Acid Sequence Antigens Base Sequence binding Binding Sites Biological and medical sciences Bw62 determinant Cell Line DNA Fundamental and applied biological sciences. Psychology Fundamental immunology genetic variance histocompatibility antigen HLA Histocompatibility antigens (hla, h-2 and other systems) HLA-B Antigens - chemistry HLA-B Antigens - genetics HLA-B Antigens - immunology HLA-B15 Antigen Humans Isoelectric Focusing man Molecular immunology Molecular Sequence Data Phenotype Protein Structure, Tertiary sites T-Lymphocytes, Cytotoxic - immunology |
| title | Allelic variations clustered in the antigen binding sites of HLA-Bw62 molecules |
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