An analysis of the genome of actinophage φEC

The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by dige...

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Veröffentlicht in:Gene 1980-12, Vol.12 (3), p.311-314
Hauptverfasser: Brownell, George H., Enquist, Lynn W., Denniston-Thompson, Katherine
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Enquist, Lynn W.
Denniston-Thompson, Katherine
description The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes BamHI, HpaI, PvuII, XbaI and XhoI were used to construct a map of the φEC genome.
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subjects Bacteriophages - genetics
Chromosome Mapping
DNA Restriction Enzymes - metabolism
DNA, Viral - analysis
DNA, Viral - metabolism
electron microscopy of DNA
Microscopy, Electron
Nocardia
Nucleic Acid Conformation
Restriction endonuclease mapping
Rhodococcus
title An analysis of the genome of actinophage φEC
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