An analysis of the genome of actinophage φEC
The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by dige...
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Veröffentlicht in: | Gene 1980-12, Vol.12 (3), p.311-314 |
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creator | Brownell, George H. Enquist, Lynn W. Denniston-Thompson, Katherine |
description | The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes
BamHI,
HpaI,
PvuII,
XbaI and
XhoI were used to construct a map of the φEC genome. |
doi_str_mv | 10.1016/0378-1119(80)90114-6 |
format | Article |
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BamHI,
HpaI,
PvuII,
XbaI and
XhoI were used to construct a map of the φEC genome.</description><identifier>ISSN: 0378-1119</identifier><identifier>EISSN: 1879-0038</identifier><identifier>DOI: 10.1016/0378-1119(80)90114-6</identifier><identifier>PMID: 6265324</identifier><language>eng</language><publisher>Netherlands: Elsevier B.V</publisher><subject>Bacteriophages - genetics ; Chromosome Mapping ; DNA Restriction Enzymes - metabolism ; DNA, Viral - analysis ; DNA, Viral - metabolism ; electron microscopy of DNA ; Microscopy, Electron ; Nocardia ; Nucleic Acid Conformation ; Restriction endonuclease mapping ; Rhodococcus</subject><ispartof>Gene, 1980-12, Vol.12 (3), p.311-314</ispartof><rights>1980</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c272t-c101a366919105d5dd7b5471523e2278a6c3902bc4f0101835e71f77e22d8b953</citedby><cites>FETCH-LOGICAL-c272t-c101a366919105d5dd7b5471523e2278a6c3902bc4f0101835e71f77e22d8b953</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://dx.doi.org/10.1016/0378-1119(80)90114-6$$EHTML$$P50$$Gelsevier$$H</linktohtml><link.rule.ids>314,780,784,3550,27924,27925,45995</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/6265324$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Brownell, George H.</creatorcontrib><creatorcontrib>Enquist, Lynn W.</creatorcontrib><creatorcontrib>Denniston-Thompson, Katherine</creatorcontrib><title>An analysis of the genome of actinophage φEC</title><title>Gene</title><addtitle>Gene</addtitle><description>The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes
BamHI,
HpaI,
PvuII,
XbaI and
XhoI were used to construct a map of the φEC genome.</description><subject>Bacteriophages - genetics</subject><subject>Chromosome Mapping</subject><subject>DNA Restriction Enzymes - metabolism</subject><subject>DNA, Viral - analysis</subject><subject>DNA, Viral - metabolism</subject><subject>electron microscopy of DNA</subject><subject>Microscopy, Electron</subject><subject>Nocardia</subject><subject>Nucleic Acid Conformation</subject><subject>Restriction endonuclease mapping</subject><subject>Rhodococcus</subject><issn>0378-1119</issn><issn>1879-0038</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1980</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp9kMtKw0AUhgdRaq2-gUJWoovonJnMJRuhlHqBghtdD5PJSTuSS82kQl_A1_OVTGzp0rM5HP7_3D5CLoHeAQV5T7nSMQCkN5rephQgieURGYNWaUwp18dkfLCckrMQPmgfQrARGUkmBWfJmMTTOrK1LbfBh6gpom6F0RLrpsKhsq7zdbNe2SVGP9_z2Tk5KWwZ8GKfJ-T9cf42e44Xr08vs-kidkyxLnb9fZZLmUIKVOQiz1UmEgWCcWRMaSsdTynLXFLQ3qq5QAWFUr2Y6ywVfEKud3PXbfO5wdCZygeHZWlrbDbBKCE4FzAYk53RtU0ILRZm3frKtlsD1AyUzIDADAiMpuaPkpF929V-_iarMD807bH0-sNOx_7JL4-tCc5j7TD3LbrO5I3_f8EvG3pzNQ</recordid><startdate>198012</startdate><enddate>198012</enddate><creator>Brownell, George H.</creator><creator>Enquist, Lynn W.</creator><creator>Denniston-Thompson, Katherine</creator><general>Elsevier B.V</general><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>198012</creationdate><title>An analysis of the genome of actinophage φEC</title><author>Brownell, George H. ; Enquist, Lynn W. ; Denniston-Thompson, Katherine</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c272t-c101a366919105d5dd7b5471523e2278a6c3902bc4f0101835e71f77e22d8b953</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1980</creationdate><topic>Bacteriophages - genetics</topic><topic>Chromosome Mapping</topic><topic>DNA Restriction Enzymes - metabolism</topic><topic>DNA, Viral - analysis</topic><topic>DNA, Viral - metabolism</topic><topic>electron microscopy of DNA</topic><topic>Microscopy, Electron</topic><topic>Nocardia</topic><topic>Nucleic Acid Conformation</topic><topic>Restriction endonuclease mapping</topic><topic>Rhodococcus</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Brownell, George H.</creatorcontrib><creatorcontrib>Enquist, Lynn W.</creatorcontrib><creatorcontrib>Denniston-Thompson, Katherine</creatorcontrib><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>Gene</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Brownell, George H.</au><au>Enquist, Lynn W.</au><au>Denniston-Thompson, Katherine</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>An analysis of the genome of actinophage φEC</atitle><jtitle>Gene</jtitle><addtitle>Gene</addtitle><date>1980-12</date><risdate>1980</risdate><volume>12</volume><issue>3</issue><spage>311</spage><epage>314</epage><pages>311-314</pages><issn>0378-1119</issn><eissn>1879-0038</eissn><abstract>The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes
BamHI,
HpaI,
PvuII,
XbaI and
XhoI were used to construct a map of the φEC genome.</abstract><cop>Netherlands</cop><pub>Elsevier B.V</pub><pmid>6265324</pmid><doi>10.1016/0378-1119(80)90114-6</doi><tpages>4</tpages></addata></record> |
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subjects | Bacteriophages - genetics Chromosome Mapping DNA Restriction Enzymes - metabolism DNA, Viral - analysis DNA, Viral - metabolism electron microscopy of DNA Microscopy, Electron Nocardia Nucleic Acid Conformation Restriction endonuclease mapping Rhodococcus |
title | An analysis of the genome of actinophage φEC |
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