An analysis of the genome of actinophage φEC

The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by dige...

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Veröffentlicht in:	Gene 1980-12, Vol.12 (3), p.311-314
Hauptverfasser:	Brownell, George H., Enquist, Lynn W., Denniston-Thompson, Katherine
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacteriophages - genetics Chromosome Mapping DNA Restriction Enzymes - metabolism DNA, Viral - analysis DNA, Viral - metabolism electron microscopy of DNA Microscopy, Electron Nocardia Nucleic Acid Conformation Restriction endonuclease mapping Rhodococcus
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Zusammenfassung:	The DNA of actinophage φEC was characterized by physical and enzymatic methods. In the electron microscope, the DNA appeared as a linear, double-stranded molecule with an average chain length of 43.2 ± 1.7 kb. Further studies suggested that the DNA carried cohesive ends. The DNA was analyzed by digestion with 32 restriction endonucleases. The enzymes BamHI, HpaI, PvuII, XbaI and XhoI were used to construct a map of the φEC genome.
ISSN:	0378-1119 1879-0038
DOI:	10.1016/0378-1119(80)90114-6