Rapid and protracted phases of retinal ganglion cell loss follow axotomy in the optic nerve of adult rats
To investigate the short‐and long‐term effects of axotomy on the survival of central nervous system (CNS) neurons in adult rats, retinal ganglion cells (RGCs) were labelled retrogradely with the persistent market diI and their axons interrupted in the optic nerve (ON) by intracranial crush 8 or 10 m...
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Veröffentlicht in: | Journal of neurobiology 1993-01, Vol.24 (1), p.23-36 |
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description | To investigate the short‐and long‐term effects of axotomy on the survival of central nervous system (CNS) neurons in adult rats, retinal ganglion cells (RGCs) were labelled retrogradely with the persistent market diI and their axons interrupted in the optic nerve (ON) by intracranial crush 8 or 10 mm from the eye or in intraorbital cut 0.5 or 3 mm from the eye. Labelled RGCs were counted in flat‐mounted retinas at intervals from 2 weeks to 20 months after axotomy. Two major patterns of RGC loss were observed: (1) an inital abrupt loss that was confined to the first 2 weeks after injury and was more severe when the ON was cut close to the eye; (2) a slower, persistent decline in RGC densities with one‐half survival times that ranged from approximately 1 month after intraorbital ON cut to 6 months after intracranial ON crush. A small population of RGCs (approximately 5%) survived for as long as 20 months after intraorbital axotomy. The initial loss of axotomized RGCs presumably results from time‐limited perturbations related to the position of the ON injury. A. persistent lack of terminal connectivity between RGCs and their targets in the brain may contribute to the subsequent, more protracted RGC loss, but the differences between intraorbital cut and intracranial crush suggest that additional mechanisms are involved. It is unclear whether the various injury‐related processes set in motion in both the ON and the retina exert random effects on all RGCs or act preferentially on subpopulations of these neurons. © 1993 John Wiley & Sons, Inc. |
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Labelled RGCs were counted in flat‐mounted retinas at intervals from 2 weeks to 20 months after axotomy. Two major patterns of RGC loss were observed: (1) an inital abrupt loss that was confined to the first 2 weeks after injury and was more severe when the ON was cut close to the eye; (2) a slower, persistent decline in RGC densities with one‐half survival times that ranged from approximately 1 month after intraorbital ON cut to 6 months after intracranial ON crush. A small population of RGCs (approximately 5%) survived for as long as 20 months after intraorbital axotomy. The initial loss of axotomized RGCs presumably results from time‐limited perturbations related to the position of the ON injury. A. persistent lack of terminal connectivity between RGCs and their targets in the brain may contribute to the subsequent, more protracted RGC loss, but the differences between intraorbital cut and intracranial crush suggest that additional mechanisms are involved. It is unclear whether the various injury‐related processes set in motion in both the ON and the retina exert random effects on all RGCs or act preferentially on subpopulations of these neurons. © 1993 John Wiley & Sons, Inc.</description><identifier>ISSN: 0022-3034</identifier><identifier>EISSN: 1097-4695</identifier><identifier>DOI: 10.1002/neu.480240103</identifier><identifier>PMID: 8419522</identifier><identifier>CODEN: JNEUBZ</identifier><language>eng</language><publisher>Hoboken: Wiley Subscription Services, Inc., A Wiley Company</publisher><subject>Animals ; Axons - physiology ; axotomy ; Biological and medical sciences ; Cell Count ; Cell Survival - physiology ; DiI‐labelled RGCs ; Eye and associated structures. Visual pathways and centers. Vision ; Female ; Fundamental and applied biological sciences. Psychology ; Optic Disk ; Optic Nerve - physiology ; Optic Nerve - ultrastructure ; optic nerve injury ; Rats ; Rats, Sprague-Dawley ; Reference Values ; retinal ganglion cell death ; Retinal Ganglion Cells - cytology ; Time Factors ; Vertebrates: nervous system and sense organs</subject><ispartof>Journal of neurobiology, 1993-01, Vol.24 (1), p.23-36</ispartof><rights>Copyright © 1993 John Wiley & Sons, Inc.</rights><rights>1993 INIST-CNRS</rights><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c4623-f50c56f91e8d99e4ff6789e10e799b685e44f9dcd453f4de4b22de55373cdcf83</citedby><cites>FETCH-LOGICAL-c4623-f50c56f91e8d99e4ff6789e10e799b685e44f9dcd453f4de4b22de55373cdcf83</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://onlinelibrary.wiley.com/doi/pdf/10.1002%2Fneu.480240103$$EPDF$$P50$$Gwiley$$H</linktopdf><linktohtml>$$Uhttps://onlinelibrary.wiley.com/doi/full/10.1002%2Fneu.480240103$$EHTML$$P50$$Gwiley$$H</linktohtml><link.rule.ids>314,780,784,1417,4024,27923,27924,27925,45574,45575</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=4458083$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8419522$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Villegas‐Pérez, Maria‐Paz</creatorcontrib><creatorcontrib>Vidal‐Sanz, Manuel</creatorcontrib><creatorcontrib>Rasminsky, Michael</creatorcontrib><creatorcontrib>Bray, Garth M.</creatorcontrib><creatorcontrib>Aguayo, Albert J.</creatorcontrib><title>Rapid and protracted phases of retinal ganglion cell loss follow axotomy in the optic nerve of adult rats</title><title>Journal of neurobiology</title><addtitle>J Neurobiol</addtitle><description>To investigate the short‐and long‐term effects of axotomy on the survival of central nervous system (CNS) neurons in adult rats, retinal ganglion cells (RGCs) were labelled retrogradely with the persistent market diI and their axons interrupted in the optic nerve (ON) by intracranial crush 8 or 10 mm from the eye or in intraorbital cut 0.5 or 3 mm from the eye. Labelled RGCs were counted in flat‐mounted retinas at intervals from 2 weeks to 20 months after axotomy. Two major patterns of RGC loss were observed: (1) an inital abrupt loss that was confined to the first 2 weeks after injury and was more severe when the ON was cut close to the eye; (2) a slower, persistent decline in RGC densities with one‐half survival times that ranged from approximately 1 month after intraorbital ON cut to 6 months after intracranial ON crush. A small population of RGCs (approximately 5%) survived for as long as 20 months after intraorbital axotomy. The initial loss of axotomized RGCs presumably results from time‐limited perturbations related to the position of the ON injury. A. persistent lack of terminal connectivity between RGCs and their targets in the brain may contribute to the subsequent, more protracted RGC loss, but the differences between intraorbital cut and intracranial crush suggest that additional mechanisms are involved. It is unclear whether the various injury‐related processes set in motion in both the ON and the retina exert random effects on all RGCs or act preferentially on subpopulations of these neurons. © 1993 John Wiley & Sons, Inc.</description><subject>Animals</subject><subject>Axons - physiology</subject><subject>axotomy</subject><subject>Biological and medical sciences</subject><subject>Cell Count</subject><subject>Cell Survival - physiology</subject><subject>DiI‐labelled RGCs</subject><subject>Eye and associated structures. Visual pathways and centers. Vision</subject><subject>Female</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Optic Disk</subject><subject>Optic Nerve - physiology</subject><subject>Optic Nerve - ultrastructure</subject><subject>optic nerve injury</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Reference Values</subject><subject>retinal ganglion cell death</subject><subject>Retinal Ganglion Cells - cytology</subject><subject>Time Factors</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-3034</issn><issn>1097-4695</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkb1vFDEQxS0ECkegpERygeg2-HN3XaIoJEgRkaKkXvnscWLksw_bm3D_fby609FB5ZHeb55n5iH0kZIzSgj7GmE-EyNhglDCX6EVJWroRK_ka7RqOus44eItelfKL0KIUpKdoJNR0FawFfK3eust1tHibU41a1OhlY-6QMHJ4QzVRx3wg44PwaeIDYSAQyoFuxRCesb6T6pps8M-4voIOG2rNzhCfoKlX9s5VJx1Le_RG6dDgQ-H9xTdf7-4O7_qrm8uf5x_u-6M6BnvnCRG9k5RGK1SIJzrh1EBJTAote5HCUI4ZY0VkjthQawZsyAlH7ixxo38FH3Z-7Z9fs9Q6rTxZZlaR0hzmYbGMiXIf0HaS057uoDdHjS57Z3BTdvsNzrvJkqmJYOpZTAdM2j8p4PxvN6APdKHozf980HXxejgso7GlyMmhBzJuNgMe-zZB9j9-8_p58X93wFeAA1Zn-Q</recordid><startdate>199301</startdate><enddate>199301</enddate><creator>Villegas‐Pérez, Maria‐Paz</creator><creator>Vidal‐Sanz, Manuel</creator><creator>Rasminsky, Michael</creator><creator>Bray, Garth M.</creator><creator>Aguayo, Albert J.</creator><general>Wiley Subscription Services, Inc., A Wiley Company</general><general>Wiley</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7TK</scope><scope>7X8</scope></search><sort><creationdate>199301</creationdate><title>Rapid and protracted phases of retinal ganglion cell loss follow axotomy in the optic nerve of adult rats</title><author>Villegas‐Pérez, Maria‐Paz ; Vidal‐Sanz, Manuel ; Rasminsky, Michael ; Bray, Garth M. ; Aguayo, Albert J.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c4623-f50c56f91e8d99e4ff6789e10e799b685e44f9dcd453f4de4b22de55373cdcf83</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Animals</topic><topic>Axons - physiology</topic><topic>axotomy</topic><topic>Biological and medical sciences</topic><topic>Cell Count</topic><topic>Cell Survival - physiology</topic><topic>DiI‐labelled RGCs</topic><topic>Eye and associated structures. Visual pathways and centers. Vision</topic><topic>Female</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Optic Disk</topic><topic>Optic Nerve - physiology</topic><topic>Optic Nerve - ultrastructure</topic><topic>optic nerve injury</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Reference Values</topic><topic>retinal ganglion cell death</topic><topic>Retinal Ganglion Cells - cytology</topic><topic>Time Factors</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>online_resources</toplevel><creatorcontrib>Villegas‐Pérez, Maria‐Paz</creatorcontrib><creatorcontrib>Vidal‐Sanz, Manuel</creatorcontrib><creatorcontrib>Rasminsky, Michael</creatorcontrib><creatorcontrib>Bray, Garth M.</creatorcontrib><creatorcontrib>Aguayo, Albert J.</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Neurosciences Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Journal of neurobiology</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Villegas‐Pérez, Maria‐Paz</au><au>Vidal‐Sanz, Manuel</au><au>Rasminsky, Michael</au><au>Bray, Garth M.</au><au>Aguayo, Albert J.</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Rapid and protracted phases of retinal ganglion cell loss follow axotomy in the optic nerve of adult rats</atitle><jtitle>Journal of neurobiology</jtitle><addtitle>J Neurobiol</addtitle><date>1993-01</date><risdate>1993</risdate><volume>24</volume><issue>1</issue><spage>23</spage><epage>36</epage><pages>23-36</pages><issn>0022-3034</issn><eissn>1097-4695</eissn><coden>JNEUBZ</coden><abstract>To investigate the short‐and long‐term effects of axotomy on the survival of central nervous system (CNS) neurons in adult rats, retinal ganglion cells (RGCs) were labelled retrogradely with the persistent market diI and their axons interrupted in the optic nerve (ON) by intracranial crush 8 or 10 mm from the eye or in intraorbital cut 0.5 or 3 mm from the eye. Labelled RGCs were counted in flat‐mounted retinas at intervals from 2 weeks to 20 months after axotomy. Two major patterns of RGC loss were observed: (1) an inital abrupt loss that was confined to the first 2 weeks after injury and was more severe when the ON was cut close to the eye; (2) a slower, persistent decline in RGC densities with one‐half survival times that ranged from approximately 1 month after intraorbital ON cut to 6 months after intracranial ON crush. A small population of RGCs (approximately 5%) survived for as long as 20 months after intraorbital axotomy. The initial loss of axotomized RGCs presumably results from time‐limited perturbations related to the position of the ON injury. A. persistent lack of terminal connectivity between RGCs and their targets in the brain may contribute to the subsequent, more protracted RGC loss, but the differences between intraorbital cut and intracranial crush suggest that additional mechanisms are involved. It is unclear whether the various injury‐related processes set in motion in both the ON and the retina exert random effects on all RGCs or act preferentially on subpopulations of these neurons. © 1993 John Wiley & Sons, Inc.</abstract><cop>Hoboken</cop><pub>Wiley Subscription Services, Inc., A Wiley Company</pub><pmid>8419522</pmid><doi>10.1002/neu.480240103</doi><tpages>14</tpages></addata></record> |
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subjects | Animals Axons - physiology axotomy Biological and medical sciences Cell Count Cell Survival - physiology DiI‐labelled RGCs Eye and associated structures. Visual pathways and centers. Vision Female Fundamental and applied biological sciences. Psychology Optic Disk Optic Nerve - physiology Optic Nerve - ultrastructure optic nerve injury Rats Rats, Sprague-Dawley Reference Values retinal ganglion cell death Retinal Ganglion Cells - cytology Time Factors Vertebrates: nervous system and sense organs |
title | Rapid and protracted phases of retinal ganglion cell loss follow axotomy in the optic nerve of adult rats |
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