Single pass sequencing and physical and genetic mapping of human brain cDNAs
We have performed single pass sequencing of 1,024 human brain cDNAs, over 900 of which seem to represent new human genes. Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chro...
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| Veröffentlicht in: | Nature genetics 1992-11, Vol.2 (3), p.180-185 |
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| container_title | Nature genetics |
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| creator | Khan, A.S Wilcox, A.S Polymeropoulos, M.H Hopkins, J.A Stevens, T.J Robinson, M Orpana, A.K Sikela, J.M |
| description | We have performed single pass sequencing of 1,024 human brain cDNAs, over 900 of which seem to represent new human genes. Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chromosomal locations using gene–specific STS primers derived from 3′ untranslated regions. We have also determined that human brain cDNAs represent a rich source of gene–associated polymorphic markers. Microsatellite–containing cDNAs can be physically mapped and converted to highly informative genetic markers, thus facilitating integration of the human physical, expression and genetic maps. |
| doi_str_mv | 10.1038/ng1192-180 |
| format | Article |
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Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chromosomal locations using gene–specific STS primers derived from 3′ untranslated regions. We have also determined that human brain cDNAs represent a rich source of gene–associated polymorphic markers. 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Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chromosomal locations using gene–specific STS primers derived from 3′ untranslated regions. We have also determined that human brain cDNAs represent a rich source of gene–associated polymorphic markers. 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Library prescreening with total brain cDNA significantly reduced repeated sequencing of highly represented cDNAs. A subset of sequenced cDNAs were physically mapped to their chromosomal locations using gene–specific STS primers derived from 3′ untranslated regions. We have also determined that human brain cDNAs represent a rich source of gene–associated polymorphic markers. Microsatellite–containing cDNAs can be physically mapped and converted to highly informative genetic markers, thus facilitating integration of the human physical, expression and genetic maps.</abstract><cop>New York</cop><pub>Nature Publishing Group US</pub><pmid>1345165</pmid><doi>10.1038/ng1192-180</doi><tpages>6</tpages></addata></record> |
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| subjects | Agriculture Animal Genetics and Genomics Base Sequence Biomedical and Life Sciences Biomedicine brain Brain Chemistry - genetics Cancer Research cDNA Child, Preschool Chromosome Mapping chromosomes Cloning, Molecular complementary DNA Databases, Factual DNA DNA libraries DNA, Complementary - genetics DNA, Satellite - analysis Female Fetus Gene Function Gene Library gene location gene mapping genes genetic markers genetic polymorphism Human Genetics Humans Infant man Molecular Sequence Data nucleotide sequences Polymorphism, Genetic Proteins - genetics Repetitive Sequences, Nucleic Acid - genetics sequence sequence analysis Sequence Analysis, DNA - methods Sequence Homology, Nucleic Acid Spinal Muscular Atrophies of Childhood - genetics |
| title | Single pass sequencing and physical and genetic mapping of human brain cDNAs |
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