Cryopreservation of mammalian embryos: Advancement of putting life on hold

Rodent transgenesis and human‐assisted reproductive programs involve multistep handling of preimplantation embryos. The efficacy of production and quality of results from conventionally scheduled programs are limited by temporal constraints other than the quality and quantities of embryos per se. Th...

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Veröffentlicht in:Birth defects research. Part C. Embryo today 2010-09, Vol.90 (3), p.163-175
Hauptverfasser: Tsang, Wai Hung, Chow, King L.
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container_title Birth defects research. Part C. Embryo today
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creator Tsang, Wai Hung
Chow, King L.
description Rodent transgenesis and human‐assisted reproductive programs involve multistep handling of preimplantation embryos. The efficacy of production and quality of results from conventionally scheduled programs are limited by temporal constraints other than the quality and quantities of embryos per se. The emergence of vitrification, a water ice‐free cryopreservation technique, as a reliable way to arrest further growth of preimplantation embryos, provides an option to eliminate the time constraint. In this article, current and potential applications of cryopreservation to facilitate laboratory animal experiments, colony management, and human‐assisted reproductive programs are reviewed. Carrier devices developed for vitrification in the last two decades are compared with an emphasis on their physical properties that infer cooling rate of samples and sterility assurance. Biological impacts of improved cryopreservation on preimplantation embryos are also discussed. Birth Defects Research (Part C) 90:163–175, 2010. © 2010 Wiley‐Liss, Inc.
doi_str_mv 10.1002/bdrc.20186
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subjects animal colony management
Animals
assisted reproductive technology
Blastocyst - metabolism
Cryopreservation
Embryo, Mammalian - embryology
Female
Gene Transfer Techniques
Humans
Mice
Models, Animal
Reproductive Techniques, Assisted
transgenesis
Vitrification
title Cryopreservation of mammalian embryos: Advancement of putting life on hold
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