Utilizing Three Monoclonal Antibodies in the Development of an Immunochromatographic Assay for Simultaneous Detection of Sulfamethazine, Sulfadiazine, and Sulfaquinoxaline Residues in Egg and Chicken Muscle
A rapid and sensitive immunochromatographic assay (ICA) based on competitive format was developed and validated for simultaneous detection of sulfamethazine (SM2), sulfadiazine (SDZ), and sulfaquinoxaline (SQX) in chicken breast muscle and egg samples. For this purpose, three monoclonal antibodies r...
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| Veröffentlicht in: | Analytical chemistry (Washington) 2010-09, Vol.82 (18), p.7550-7555 |
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| creator | Guo, Yancheng Ngom, Babacar Le, Tao Jin, Xiue Wang, Liping Shi, Deshi Wang, Xiliang Bi, Dingren |
| description | A rapid and sensitive immunochromatographic assay (ICA) based on competitive format was developed and validated for simultaneous detection of sulfamethazine (SM2), sulfadiazine (SDZ), and sulfaquinoxaline (SQX) in chicken breast muscle and egg samples. For this purpose, three monoclonal antibodies raised against those three sulfonamides were conjugated to colloidal gold particles and applied to the conjugate pads of the test strip. The competitors of the sulfonamides (SM2/SDZ/SQX−bovine serum albumin conjugates) were immobilized onto a nitrocellulose membrane at three detection zones to form T1, T2, and T3, respectively. With this method, the cutoff values for the three test lines were achieved at 80 μg/kg, which is lower than the maximum residue levels (MRLs) established for sulfonamides. The recoveries in negative samples spiked at concentrations of 10, 50, and 100 μg/kg ranged from 75% to 82% for egg samples and from 78% to 81% for chicken samples. The method was compared with the HPLC method by testing 180 eggs and chicken breast samples from local markets, and an agreement rate of 99.7% was obtained between the two methods. |
| doi_str_mv | 10.1021/ac101020y |
| format | Article |
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For this purpose, three monoclonal antibodies raised against those three sulfonamides were conjugated to colloidal gold particles and applied to the conjugate pads of the test strip. The competitors of the sulfonamides (SM2/SDZ/SQX−bovine serum albumin conjugates) were immobilized onto a nitrocellulose membrane at three detection zones to form T1, T2, and T3, respectively. With this method, the cutoff values for the three test lines were achieved at 80 μg/kg, which is lower than the maximum residue levels (MRLs) established for sulfonamides. The recoveries in negative samples spiked at concentrations of 10, 50, and 100 μg/kg ranged from 75% to 82% for egg samples and from 78% to 81% for chicken samples. The method was compared with the HPLC method by testing 180 eggs and chicken breast samples from local markets, and an agreement rate of 99.7% was obtained between the two methods.</description><identifier>ISSN: 0003-2700</identifier><identifier>EISSN: 1520-6882</identifier><identifier>DOI: 10.1021/ac101020y</identifier><identifier>PMID: 20726505</identifier><identifier>CODEN: ANCHAM</identifier><language>eng</language><publisher>Washington, DC: American Chemical Society</publisher><subject>Analytic Sample Preparation Methods ; Analytical chemistry ; Animals ; Antibodies, Monoclonal - immunology ; Bioassays ; Cattle ; Chemistry ; Chickens ; Chromatographic methods and physical methods associated with chromatography ; Chromatography - methods ; Chromatography, High Pressure Liquid ; Drug Residues - analysis ; Eggs ; Electrochemical methods ; Exact sciences and technology ; Food Contamination - analysis ; Gold Colloid - chemistry ; Immunoassay - methods ; Meat - analysis ; Muscles - chemistry ; Muscular system ; Other chromatographic methods ; Ovum - chemistry ; Poultry ; Sulfadiazine - analysis ; Sulfadiazine - immunology ; Sulfamethazine - analysis ; Sulfamethazine - immunology ; Sulfaquinoxaline - analysis ; Sulfaquinoxaline - immunology ; Time Factors</subject><ispartof>Analytical chemistry (Washington), 2010-09, Vol.82 (18), p.7550-7555</ispartof><rights>Copyright © 2010 American Chemical Society</rights><rights>2015 INIST-CNRS</rights><rights>Copyright American Chemical Society Sep 15, 2010</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-a371t-f8ed468aaa9275ace8f9be8a7ff3f301f17570eb5750689b1d74203c3ead24cd3</citedby><cites>FETCH-LOGICAL-a371t-f8ed468aaa9275ace8f9be8a7ff3f301f17570eb5750689b1d74203c3ead24cd3</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktopdf>$$Uhttps://pubs.acs.org/doi/pdf/10.1021/ac101020y$$EPDF$$P50$$Gacs$$H</linktopdf><linktohtml>$$Uhttps://pubs.acs.org/doi/10.1021/ac101020y$$EHTML$$P50$$Gacs$$H</linktohtml><link.rule.ids>314,776,780,2752,27053,27901,27902,56713,56763</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=23232624$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20726505$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Guo, Yancheng</creatorcontrib><creatorcontrib>Ngom, Babacar</creatorcontrib><creatorcontrib>Le, Tao</creatorcontrib><creatorcontrib>Jin, Xiue</creatorcontrib><creatorcontrib>Wang, Liping</creatorcontrib><creatorcontrib>Shi, Deshi</creatorcontrib><creatorcontrib>Wang, Xiliang</creatorcontrib><creatorcontrib>Bi, Dingren</creatorcontrib><title>Utilizing Three Monoclonal Antibodies in the Development of an Immunochromatographic Assay for Simultaneous Detection of Sulfamethazine, Sulfadiazine, and Sulfaquinoxaline Residues in Egg and Chicken Muscle</title><title>Analytical chemistry (Washington)</title><addtitle>Anal. Chem</addtitle><description>A rapid and sensitive immunochromatographic assay (ICA) based on competitive format was developed and validated for simultaneous detection of sulfamethazine (SM2), sulfadiazine (SDZ), and sulfaquinoxaline (SQX) in chicken breast muscle and egg samples. For this purpose, three monoclonal antibodies raised against those three sulfonamides were conjugated to colloidal gold particles and applied to the conjugate pads of the test strip. The competitors of the sulfonamides (SM2/SDZ/SQX−bovine serum albumin conjugates) were immobilized onto a nitrocellulose membrane at three detection zones to form T1, T2, and T3, respectively. With this method, the cutoff values for the three test lines were achieved at 80 μg/kg, which is lower than the maximum residue levels (MRLs) established for sulfonamides. The recoveries in negative samples spiked at concentrations of 10, 50, and 100 μg/kg ranged from 75% to 82% for egg samples and from 78% to 81% for chicken samples. The method was compared with the HPLC method by testing 180 eggs and chicken breast samples from local markets, and an agreement rate of 99.7% was obtained between the two methods.</description><subject>Analytic Sample Preparation Methods</subject><subject>Analytical chemistry</subject><subject>Animals</subject><subject>Antibodies, Monoclonal - immunology</subject><subject>Bioassays</subject><subject>Cattle</subject><subject>Chemistry</subject><subject>Chickens</subject><subject>Chromatographic methods and physical methods associated with chromatography</subject><subject>Chromatography - methods</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Drug Residues - analysis</subject><subject>Eggs</subject><subject>Electrochemical methods</subject><subject>Exact sciences and technology</subject><subject>Food Contamination - analysis</subject><subject>Gold Colloid - chemistry</subject><subject>Immunoassay - methods</subject><subject>Meat - analysis</subject><subject>Muscles - chemistry</subject><subject>Muscular system</subject><subject>Other chromatographic methods</subject><subject>Ovum - chemistry</subject><subject>Poultry</subject><subject>Sulfadiazine - analysis</subject><subject>Sulfadiazine - immunology</subject><subject>Sulfamethazine - analysis</subject><subject>Sulfamethazine - immunology</subject><subject>Sulfaquinoxaline - analysis</subject><subject>Sulfaquinoxaline - immunology</subject><subject>Time Factors</subject><issn>0003-2700</issn><issn>1520-6882</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNplkV1rFDEUhgdR7Fq98A9IEEQER5PMR2Yvl7VqoUWw7fVwJnOyk5pJtklGXH-kv8mss3ZByUVyDs97PvJm2XNG3zHK2XuQjKYH3T3IFqziNK-bhj_MFpTSIueC0pPsSQi3lLLE1Y-zE04FrytaLbJfN1Eb_VPbDbkePCK5dNZJ4ywYsrJRd67XGIi2JA5IPuB3NG47oo3EKQKWnI_jlASDdyNEt_GwHbQkqxBgR5Tz5EqPk4lg0U0hySPKqJ3di68mo2DEOEDqjm_nuNeHCGw_Z-4mbd0PMClLvmLQ_TSPc7bZ_IHWqd83tORyCtLg0-yRAhPw2eE-zW4-nl2vP-cXXz6dr1cXORSCxVw12Jd1AwBLLiqQ2Khlhw0IpQpVUKaYqATFrhIVrZtlx3pRclrIAqHnpeyL0-z1XHfr3V2aKLajDhKNmTdtRVUxsWR1k8iX_5C3bvLpe_eQoHXJWZ2gNzMkvQvBo2q3Xo_gdy2j7d7i9t7ixL44FJy6Eft78q-nCXh1ACBIMMqDlTocuSKdmpdHDmQ4DvV_w984F758</recordid><startdate>20100915</startdate><enddate>20100915</enddate><creator>Guo, Yancheng</creator><creator>Ngom, Babacar</creator><creator>Le, Tao</creator><creator>Jin, Xiue</creator><creator>Wang, Liping</creator><creator>Shi, Deshi</creator><creator>Wang, Xiliang</creator><creator>Bi, Dingren</creator><general>American Chemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7QF</scope><scope>7QO</scope><scope>7QQ</scope><scope>7SC</scope><scope>7SE</scope><scope>7SP</scope><scope>7SR</scope><scope>7TA</scope><scope>7TB</scope><scope>7TM</scope><scope>7U5</scope><scope>7U7</scope><scope>7U9</scope><scope>8BQ</scope><scope>8FD</scope><scope>C1K</scope><scope>F28</scope><scope>FR3</scope><scope>H8D</scope><scope>H8G</scope><scope>H94</scope><scope>JG9</scope><scope>JQ2</scope><scope>KR7</scope><scope>L7M</scope><scope>L~C</scope><scope>L~D</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>20100915</creationdate><title>Utilizing Three Monoclonal Antibodies in the Development of an Immunochromatographic Assay for Simultaneous Detection of Sulfamethazine, Sulfadiazine, and Sulfaquinoxaline Residues in Egg and Chicken Muscle</title><author>Guo, Yancheng ; Ngom, Babacar ; Le, Tao ; Jin, Xiue ; Wang, Liping ; Shi, Deshi ; Wang, Xiliang ; Bi, Dingren</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-a371t-f8ed468aaa9275ace8f9be8a7ff3f301f17570eb5750689b1d74203c3ead24cd3</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>2010</creationdate><topic>Analytic Sample Preparation Methods</topic><topic>Analytical chemistry</topic><topic>Animals</topic><topic>Antibodies, Monoclonal - immunology</topic><topic>Bioassays</topic><topic>Cattle</topic><topic>Chemistry</topic><topic>Chickens</topic><topic>Chromatographic methods and physical methods associated with chromatography</topic><topic>Chromatography - methods</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Drug Residues - analysis</topic><topic>Eggs</topic><topic>Electrochemical methods</topic><topic>Exact sciences and technology</topic><topic>Food Contamination - analysis</topic><topic>Gold Colloid - chemistry</topic><topic>Immunoassay - methods</topic><topic>Meat - analysis</topic><topic>Muscles - chemistry</topic><topic>Muscular system</topic><topic>Other chromatographic methods</topic><topic>Ovum - chemistry</topic><topic>Poultry</topic><topic>Sulfadiazine - analysis</topic><topic>Sulfadiazine - immunology</topic><topic>Sulfamethazine - analysis</topic><topic>Sulfamethazine - immunology</topic><topic>Sulfaquinoxaline - analysis</topic><topic>Sulfaquinoxaline - immunology</topic><topic>Time Factors</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Guo, Yancheng</creatorcontrib><creatorcontrib>Ngom, Babacar</creatorcontrib><creatorcontrib>Le, Tao</creatorcontrib><creatorcontrib>Jin, Xiue</creatorcontrib><creatorcontrib>Wang, Liping</creatorcontrib><creatorcontrib>Shi, Deshi</creatorcontrib><creatorcontrib>Wang, Xiliang</creatorcontrib><creatorcontrib>Bi, Dingren</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>Aluminium Industry Abstracts</collection><collection>Biotechnology Research Abstracts</collection><collection>Ceramic Abstracts</collection><collection>Computer and Information Systems Abstracts</collection><collection>Corrosion Abstracts</collection><collection>Electronics & Communications Abstracts</collection><collection>Engineered Materials Abstracts</collection><collection>Materials Business File</collection><collection>Mechanical & Transportation Engineering Abstracts</collection><collection>Nucleic Acids Abstracts</collection><collection>Solid State and Superconductivity Abstracts</collection><collection>Toxicology Abstracts</collection><collection>Virology and AIDS Abstracts</collection><collection>METADEX</collection><collection>Technology Research Database</collection><collection>Environmental Sciences and Pollution Management</collection><collection>ANTE: Abstracts in New Technology & Engineering</collection><collection>Engineering Research Database</collection><collection>Aerospace Database</collection><collection>Copper Technical Reference Library</collection><collection>AIDS and Cancer Research Abstracts</collection><collection>Materials Research Database</collection><collection>ProQuest Computer Science Collection</collection><collection>Civil Engineering Abstracts</collection><collection>Advanced Technologies Database with Aerospace</collection><collection>Computer and Information Systems Abstracts Academic</collection><collection>Computer and Information Systems Abstracts Professional</collection><collection>Biotechnology and BioEngineering Abstracts</collection><collection>MEDLINE - Academic</collection><jtitle>Analytical chemistry (Washington)</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Guo, Yancheng</au><au>Ngom, Babacar</au><au>Le, Tao</au><au>Jin, Xiue</au><au>Wang, Liping</au><au>Shi, Deshi</au><au>Wang, Xiliang</au><au>Bi, Dingren</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Utilizing Three Monoclonal Antibodies in the Development of an Immunochromatographic Assay for Simultaneous Detection of Sulfamethazine, Sulfadiazine, and Sulfaquinoxaline Residues in Egg and Chicken Muscle</atitle><jtitle>Analytical chemistry (Washington)</jtitle><addtitle>Anal. Chem</addtitle><date>2010-09-15</date><risdate>2010</risdate><volume>82</volume><issue>18</issue><spage>7550</spage><epage>7555</epage><pages>7550-7555</pages><issn>0003-2700</issn><eissn>1520-6882</eissn><coden>ANCHAM</coden><abstract>A rapid and sensitive immunochromatographic assay (ICA) based on competitive format was developed and validated for simultaneous detection of sulfamethazine (SM2), sulfadiazine (SDZ), and sulfaquinoxaline (SQX) in chicken breast muscle and egg samples. For this purpose, three monoclonal antibodies raised against those three sulfonamides were conjugated to colloidal gold particles and applied to the conjugate pads of the test strip. The competitors of the sulfonamides (SM2/SDZ/SQX−bovine serum albumin conjugates) were immobilized onto a nitrocellulose membrane at three detection zones to form T1, T2, and T3, respectively. With this method, the cutoff values for the three test lines were achieved at 80 μg/kg, which is lower than the maximum residue levels (MRLs) established for sulfonamides. The recoveries in negative samples spiked at concentrations of 10, 50, and 100 μg/kg ranged from 75% to 82% for egg samples and from 78% to 81% for chicken samples. The method was compared with the HPLC method by testing 180 eggs and chicken breast samples from local markets, and an agreement rate of 99.7% was obtained between the two methods.</abstract><cop>Washington, DC</cop><pub>American Chemical Society</pub><pmid>20726505</pmid><doi>10.1021/ac101020y</doi><tpages>6</tpages></addata></record> |
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| ispartof | Analytical chemistry (Washington), 2010-09, Vol.82 (18), p.7550-7555 |
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| source | ACS Publications; MEDLINE |
| subjects | Analytic Sample Preparation Methods Analytical chemistry Animals Antibodies, Monoclonal - immunology Bioassays Cattle Chemistry Chickens Chromatographic methods and physical methods associated with chromatography Chromatography - methods Chromatography, High Pressure Liquid Drug Residues - analysis Eggs Electrochemical methods Exact sciences and technology Food Contamination - analysis Gold Colloid - chemistry Immunoassay - methods Meat - analysis Muscles - chemistry Muscular system Other chromatographic methods Ovum - chemistry Poultry Sulfadiazine - analysis Sulfadiazine - immunology Sulfamethazine - analysis Sulfamethazine - immunology Sulfaquinoxaline - analysis Sulfaquinoxaline - immunology Time Factors |
| title | Utilizing Three Monoclonal Antibodies in the Development of an Immunochromatographic Assay for Simultaneous Detection of Sulfamethazine, Sulfadiazine, and Sulfaquinoxaline Residues in Egg and Chicken Muscle |
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