Differential visualization of cholinesterasic neuronal somata and fibers by use of modifications of acetylcholinesterase pharmacohistochemistry
We modified existing acetylcholinesterase (AChE) histochemical techniques to visualize cholinergic neuronal somata and processes in the same rat brain. The AChE staining procedure of Karnovsky and Roots, combined with diisopropylfluorophosphate (DFP) pre-treatment, have previously allowed visualizat...
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| Veröffentlicht in: | The journal of histochemistry and cytochemistry 1993-01, Vol.41 (1), p.129-135 |
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| container_title | The journal of histochemistry and cytochemistry |
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| creator | Di Patre, PL Mathes, CW Butcher, LL |
| description | We modified existing acetylcholinesterase (AChE) histochemical techniques to visualize cholinergic neuronal somata and processes in the same rat brain. The AChE staining procedure of Karnovsky and Roots, combined with diisopropylfluorophosphate (DFP) pre-treatment, have previously allowed visualization of neuronal somata. Fibers have been visualized by nickel-diaminobenzidine (DAB)-hydrogen peroxide histochemical intensification. We combined novel modifications of these techniques to visualize cell bodies and fibers simultaneously. Maximal staining of neuronal somata occurs with a 1:1 dilution of both the Karnovsky-Roots medium and the nickel-DAB solution; diluting the Karnovsky-Roots medium 1:10 and increasing the concentration of the nickel-DAB solution twofold allows visualization of fibers previously unstainable with DFP pre-treatment and the classical AChE protocol. It is now possible to visualize both neuronal somata and fibers in adjacent sections of the same brain, in a quick and inexpensive manner. |
| doi_str_mv | 10.1177/41.1.7678024 |
| format | Article |
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The AChE staining procedure of Karnovsky and Roots, combined with diisopropylfluorophosphate (DFP) pre-treatment, have previously allowed visualization of neuronal somata. Fibers have been visualized by nickel-diaminobenzidine (DAB)-hydrogen peroxide histochemical intensification. We combined novel modifications of these techniques to visualize cell bodies and fibers simultaneously. Maximal staining of neuronal somata occurs with a 1:1 dilution of both the Karnovsky-Roots medium and the nickel-DAB solution; diluting the Karnovsky-Roots medium 1:10 and increasing the concentration of the nickel-DAB solution twofold allows visualization of fibers previously unstainable with DFP pre-treatment and the classical AChE protocol. 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The AChE staining procedure of Karnovsky and Roots, combined with diisopropylfluorophosphate (DFP) pre-treatment, have previously allowed visualization of neuronal somata. Fibers have been visualized by nickel-diaminobenzidine (DAB)-hydrogen peroxide histochemical intensification. We combined novel modifications of these techniques to visualize cell bodies and fibers simultaneously. Maximal staining of neuronal somata occurs with a 1:1 dilution of both the Karnovsky-Roots medium and the nickel-DAB solution; diluting the Karnovsky-Roots medium 1:10 and increasing the concentration of the nickel-DAB solution twofold allows visualization of fibers previously unstainable with DFP pre-treatment and the classical AChE protocol. It is now possible to visualize both neuronal somata and fibers in adjacent sections of the same brain, in a quick and inexpensive manner.</description><subject>Acetylcholinesterase - analysis</subject><subject>Animals</subject><subject>Biological and medical sciences</subject><subject>Brain Chemistry</subject><subject>Central nervous system</subject><subject>Central neurotransmission. Neuromudulation. Pathways and receptors</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Isoflurophate</subject><subject>Nerve Fibers - chemistry</subject><subject>Neurons - chemistry</subject><subject>Rats</subject><subject>Rats, Sprague-Dawley</subject><subject>Staining and Labeling</subject><subject>Vertebrates: nervous system and sense organs</subject><issn>0022-1554</issn><issn>1551-5044</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1993</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNp1kM1v1DAQxS0EKtvCjStSDlAJiV3s-Cs5ovIpVeICZ2viHTeunHixk66Wf4J_GW83KuLA6Ukzv3lv9Ah5weiGMa3fCbZhG610Q2vxiKyYlGwtqRCPyYrSul6XgXhKznO-pZQJIZszcrbgK_L7g3cOE46Th1Dd-TxD8L9g8nGsoqtsH4MfMU-YIHtbjTinOBYyxwEmqGDcVs53mHLVHao54_FoiFvvvL03yccBWJwO4R8vrHY9pAFs7H2eou1xKJoOz8gTByHj80UvyI9PH79ffVlff_v89er99doKpqd1Z2kjG7GVomFNp1XNwKG13DUKW8aZwlpJqrigtGmV5qquW9kKRhXlXSMVvyCXJ99dij_n8pQp-RZDgBHjnI0uLWrZ8gK-PYE2xZwTOrNLfoB0MIyaY_9GMMPMUmjBXy6-czfg9gH-u3-17CFbCC7BaH1-wITkire6YG9OWIYbNLdxTqX0_L_I1ye29zf93ic0eYAQygPM7Pf7e5bVLf8DR0-ouQ</recordid><startdate>19930101</startdate><enddate>19930101</enddate><creator>Di Patre, PL</creator><creator>Mathes, CW</creator><creator>Butcher, LL</creator><general>Histochemical Soc</general><general>SAGE Publications</general><general>Histochemical Society</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19930101</creationdate><title>Differential visualization of cholinesterasic neuronal somata and fibers by use of modifications of acetylcholinesterase pharmacohistochemistry</title><author>Di Patre, PL ; Mathes, CW ; Butcher, LL</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-bc08584d54818b7621afecc3f86e91316e26506340089673622959410603b8563</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1993</creationdate><topic>Acetylcholinesterase - analysis</topic><topic>Animals</topic><topic>Biological and medical sciences</topic><topic>Brain Chemistry</topic><topic>Central nervous system</topic><topic>Central neurotransmission. Neuromudulation. Pathways and receptors</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Isoflurophate</topic><topic>Nerve Fibers - chemistry</topic><topic>Neurons - chemistry</topic><topic>Rats</topic><topic>Rats, Sprague-Dawley</topic><topic>Staining and Labeling</topic><topic>Vertebrates: nervous system and sense organs</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Di Patre, PL</creatorcontrib><creatorcontrib>Mathes, CW</creatorcontrib><creatorcontrib>Butcher, LL</creatorcontrib><collection>Pascal-Francis</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>The journal of histochemistry and cytochemistry</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Di Patre, PL</au><au>Mathes, CW</au><au>Butcher, LL</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>Differential visualization of cholinesterasic neuronal somata and fibers by use of modifications of acetylcholinesterase pharmacohistochemistry</atitle><jtitle>The journal of histochemistry and cytochemistry</jtitle><addtitle>J Histochem Cytochem</addtitle><date>1993-01-01</date><risdate>1993</risdate><volume>41</volume><issue>1</issue><spage>129</spage><epage>135</epage><pages>129-135</pages><issn>0022-1554</issn><eissn>1551-5044</eissn><coden>JHCYAS</coden><abstract>We modified existing acetylcholinesterase (AChE) histochemical techniques to visualize cholinergic neuronal somata and processes in the same rat brain. The AChE staining procedure of Karnovsky and Roots, combined with diisopropylfluorophosphate (DFP) pre-treatment, have previously allowed visualization of neuronal somata. Fibers have been visualized by nickel-diaminobenzidine (DAB)-hydrogen peroxide histochemical intensification. We combined novel modifications of these techniques to visualize cell bodies and fibers simultaneously. Maximal staining of neuronal somata occurs with a 1:1 dilution of both the Karnovsky-Roots medium and the nickel-DAB solution; diluting the Karnovsky-Roots medium 1:10 and increasing the concentration of the nickel-DAB solution twofold allows visualization of fibers previously unstainable with DFP pre-treatment and the classical AChE protocol. It is now possible to visualize both neuronal somata and fibers in adjacent sections of the same brain, in a quick and inexpensive manner.</abstract><cop>Los Angeles, CA</cop><pub>Histochemical Soc</pub><pmid>7678024</pmid><doi>10.1177/41.1.7678024</doi><tpages>7</tpages><oa>free_for_read</oa></addata></record> |
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| subjects | Acetylcholinesterase - analysis Animals Biological and medical sciences Brain Chemistry Central nervous system Central neurotransmission. Neuromudulation. Pathways and receptors Fundamental and applied biological sciences. Psychology Isoflurophate Nerve Fibers - chemistry Neurons - chemistry Rats Rats, Sprague-Dawley Staining and Labeling Vertebrates: nervous system and sense organs |
| title | Differential visualization of cholinesterasic neuronal somata and fibers by use of modifications of acetylcholinesterase pharmacohistochemistry |
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