The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines
The Epstein-Barr virus encoded nuclear antigen 1 (EBNA1) is required for the replication and maintenance of the episomal EBV genome and for the transactivation of viral gene expression. EBNA1 has been classified into five subtypes, among which the V-val subtype was reported to be associated with nas...
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| Veröffentlicht in: | Oncology reports 2010-05, Vol.23 (5), p.1417-1424 |
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| container_title | Oncology reports |
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| creator | MAI, Shi-Juan DAN XIE HUANG, Yu-Fan WANG, Feng-Wei LIAO, Yi-Ji DENG, Hai-Xia LIU, Wen-Ju HUA, Wen-Feng ZENG, Yi-Xin |
| description | The Epstein-Barr virus encoded nuclear antigen 1 (EBNA1) is required for the replication and maintenance of the episomal EBV genome and for the transactivation of viral gene expression. EBNA1 has been classified into five subtypes, among which the V-val subtype was reported to be associated with nasopharyngeal carcinoma (NPC). Here we report a higher transcriptional activity of the V-val subtype of EBNA1 than for the prototype derived from B95.8 cells to transactivate FR-containing luciferase plasmid, which was mainly a consequence of the mutations in the carboxy-terminus of EBNA1. This interpretation was further supported by the finding that the variant form of EBNA1 has a higher binding affinity for the FR sequence than the prototype by electrophoretic mobility shift assays. The functional advantage of the V-val EBNA1 investigated in this study may contribute to the oncogenesis of NPC. |
| doi_str_mv | 10.3892/or_00000779 |
| format | Article |
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EBNA1 has been classified into five subtypes, among which the V-val subtype was reported to be associated with nasopharyngeal carcinoma (NPC). Here we report a higher transcriptional activity of the V-val subtype of EBNA1 than for the prototype derived from B95.8 cells to transactivate FR-containing luciferase plasmid, which was mainly a consequence of the mutations in the carboxy-terminus of EBNA1. This interpretation was further supported by the finding that the variant form of EBNA1 has a higher binding affinity for the FR sequence than the prototype by electrophoretic mobility shift assays. The functional advantage of the V-val EBNA1 investigated in this study may contribute to the oncogenesis of NPC.</description><identifier>ISSN: 1021-335X</identifier><identifier>EISSN: 1791-2431</identifier><identifier>DOI: 10.3892/or_00000779</identifier><identifier>PMID: 20372859</identifier><language>eng</language><publisher>Athens: Spandidos</publisher><subject>Binding Sites ; Biological and medical sciences ; Cell Line, Transformed ; Cell Transformation, Viral - genetics ; DNA - metabolism ; Electrophoretic Mobility Shift Assay ; Epithelial Cells - metabolism ; Epstein-Barr virus ; Epstein-Barr Virus Nuclear Antigens - genetics ; Epstein-Barr Virus Nuclear Antigens - metabolism ; Gene Expression Regulation, Neoplastic ; Gene Expression Regulation, Viral ; Genes, Reporter ; Humans ; Infectious diseases ; Luciferases - biosynthesis ; Luciferases - genetics ; Medical sciences ; Mutation ; Nasopharyngeal Neoplasms - genetics ; Nasopharyngeal Neoplasms - metabolism ; Nasopharyngeal Neoplasms - virology ; Time Factors ; Transcriptional Activation ; Transfection ; Tumors ; Viral diseases</subject><ispartof>Oncology reports, 2010-05, Vol.23 (5), p.1417-1424</ispartof><rights>2015 INIST-CNRS</rights><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c387t-a5ed9343625041864a2f87a1f0cde94e13432d5f2d3782a675dac49601037a873</citedby></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,776,780,27903,27904</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=22730290$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/20372859$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>MAI, Shi-Juan</creatorcontrib><creatorcontrib>DAN XIE</creatorcontrib><creatorcontrib>HUANG, Yu-Fan</creatorcontrib><creatorcontrib>WANG, Feng-Wei</creatorcontrib><creatorcontrib>LIAO, Yi-Ji</creatorcontrib><creatorcontrib>DENG, Hai-Xia</creatorcontrib><creatorcontrib>LIU, Wen-Ju</creatorcontrib><creatorcontrib>HUA, Wen-Feng</creatorcontrib><creatorcontrib>ZENG, Yi-Xin</creatorcontrib><title>The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines</title><title>Oncology reports</title><addtitle>Oncol Rep</addtitle><description>The Epstein-Barr virus encoded nuclear antigen 1 (EBNA1) is required for the replication and maintenance of the episomal EBV genome and for the transactivation of viral gene expression. EBNA1 has been classified into five subtypes, among which the V-val subtype was reported to be associated with nasopharyngeal carcinoma (NPC). Here we report a higher transcriptional activity of the V-val subtype of EBNA1 than for the prototype derived from B95.8 cells to transactivate FR-containing luciferase plasmid, which was mainly a consequence of the mutations in the carboxy-terminus of EBNA1. This interpretation was further supported by the finding that the variant form of EBNA1 has a higher binding affinity for the FR sequence than the prototype by electrophoretic mobility shift assays. The functional advantage of the V-val EBNA1 investigated in this study may contribute to the oncogenesis of NPC.</description><subject>Binding Sites</subject><subject>Biological and medical sciences</subject><subject>Cell Line, Transformed</subject><subject>Cell Transformation, Viral - genetics</subject><subject>DNA - metabolism</subject><subject>Electrophoretic Mobility Shift Assay</subject><subject>Epithelial Cells - metabolism</subject><subject>Epstein-Barr virus</subject><subject>Epstein-Barr Virus Nuclear Antigens - genetics</subject><subject>Epstein-Barr Virus Nuclear Antigens - metabolism</subject><subject>Gene Expression Regulation, Neoplastic</subject><subject>Gene Expression Regulation, Viral</subject><subject>Genes, Reporter</subject><subject>Humans</subject><subject>Infectious diseases</subject><subject>Luciferases - biosynthesis</subject><subject>Luciferases - genetics</subject><subject>Medical sciences</subject><subject>Mutation</subject><subject>Nasopharyngeal Neoplasms - genetics</subject><subject>Nasopharyngeal Neoplasms - metabolism</subject><subject>Nasopharyngeal Neoplasms - virology</subject><subject>Time Factors</subject><subject>Transcriptional Activation</subject><subject>Transfection</subject><subject>Tumors</subject><subject>Viral diseases</subject><issn>1021-335X</issn><issn>1791-2431</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>2010</creationdate><recordtype>article</recordtype><sourceid>EIF</sourceid><recordid>eNqFkU1v2zAMhoWhw5pmO-1e6FLsUHjVh21Jxy5IP4AAvWTDbgYr06sKR3YlOUCwP18FzdrexgsJ8iFB8iXkK2ffpTbiYggN25tS5gOZcWV4IUrJj3LMBC-krH4fk5MYHxkTitXmEzkWTCqhKzMjf9cPSNE_gLfY0hTARxvcmNzgoadgk9u6tKNDR1MGfxXbnI3TfdqNuE8ux5jQ-eIHhEC3LkyR-sn2CIGCT-4Pesqp8xRHl_t7l7st9j3tncf4mXzsoI_45eDn5OfVcr24KVZ317eLy1VhpVapgApbI0tZi4qVXNcliE4r4B2zLZoSea6JtupEK5UWUKuqBVuamvF8JWgl5-Tby9wxDE8TxtRsXNyvAR6HKTaqqnh-mJH_J6XUNdPMZPL8hbRhiDFg14zBbSDsGs6avSzNO1kyfXqYO91vsH1l_-mQgbMDANFC32UdrItvnFCSCcPkMxp0lQ4</recordid><startdate>20100501</startdate><enddate>20100501</enddate><creator>MAI, Shi-Juan</creator><creator>DAN XIE</creator><creator>HUANG, Yu-Fan</creator><creator>WANG, Feng-Wei</creator><creator>LIAO, Yi-Ji</creator><creator>DENG, Hai-Xia</creator><creator>LIU, Wen-Ju</creator><creator>HUA, Wen-Feng</creator><creator>ZENG, Yi-Xin</creator><general>Spandidos</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope><scope>7TM</scope><scope>7U9</scope><scope>H94</scope></search><sort><creationdate>20100501</creationdate><title>The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines</title><author>MAI, Shi-Juan ; 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| subjects | Binding Sites Biological and medical sciences Cell Line, Transformed Cell Transformation, Viral - genetics DNA - metabolism Electrophoretic Mobility Shift Assay Epithelial Cells - metabolism Epstein-Barr virus Epstein-Barr Virus Nuclear Antigens - genetics Epstein-Barr Virus Nuclear Antigens - metabolism Gene Expression Regulation, Neoplastic Gene Expression Regulation, Viral Genes, Reporter Humans Infectious diseases Luciferases - biosynthesis Luciferases - genetics Medical sciences Mutation Nasopharyngeal Neoplasms - genetics Nasopharyngeal Neoplasms - metabolism Nasopharyngeal Neoplasms - virology Time Factors Transcriptional Activation Transfection Tumors Viral diseases |
| title | The enhanced transcriptional activity of the V-val subtype of Epstein-Barr virus nuclear antigen 1 in epithelial cell lines |
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