Autologous Feeder Cells from Embryoid Body Outgrowth Support the Long-Term Growth of Human Embryonic Stem Cells More Effectively than Those from Direct Differentiation

Autologous feeder cells have been developed by various methods to minimize the presence of xenogenic entities in human embryonic stem cell (hESC) cultures. However, there was no systematic comparison of supportive effects of the feeder cells on hESC growth, nor comparison to the supportive effects o...

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Veröffentlicht in:	Tissue engineering. Part C, Methods Methods, 2010-08, Vol.16 (4), p.719-733
Hauptverfasser:	Fu, Xin, Toh, Wei Seong, Liu, Hua, Lu, Kai, Li, Mingming, Hande, Manoor Prakash, Cao, Tong
Format:	Artikel
Sprache:	eng
Schlagworte:	Animals Cell Culture Techniques - methods Cell Differentiation Cell Line Cell Proliferation Cells Collagen - metabolism Cryopreservation Culture Media, Conditioned - metabolism Drug Combinations Embryo, Mammalian - cytology Embryo, Mammalian - metabolism Embryonic stem cells Embryonic Stem Cells - cytology Embryonic Stem Cells - metabolism Embryos Fibroblast Growth Factor 2 - secretion Fibroblast growth factors Fibroblasts - cytology Flow Cytometry Gene Expression Regulation Growth Humans Immunohistochemistry In Situ Hybridization, Fluorescence Karyotyping Laminin - metabolism Mice Properties Proteoglycans - metabolism Reverse Transcriptase Polymerase Chain Reaction Teratoma - pathology Time Factors
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