Use of interfering RNAs targeted against feline herpesvirus 1 glycoprotein D for inhibition of feline herpesvirus 1 infection of feline kidney cells

To evaluate the use of RNA interference targeted against feline herpesvirus 1 (FHV-1) glycoprotein D for inhibition of FHV-1 infection of feline kidney cells. Crandell-Rees feline kidney cells. Crandell-Rees feline kidney cells were transfected with small interfering RNAs (siRNAs) that were designed...

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Veröffentlicht in:	American journal of veterinary research 2009-08, Vol.70 (8), p.1018-1025
Hauptverfasser:	Wilkes, Rebbeca P, Kania, Stephen A
Format:	Artikel
Sprache:	eng
Schlagworte:	Analysis of Variance Animals Blotting, Western - veterinary Cats Cell Line cell lines cultured cells disease control DNA Primers - genetics Felid herpesvirus 1 Feline herpesvirus 1 Flow Cytometry - veterinary gene expression Gene Expression Regulation - genetics glycoproteins Hemagglutination Tests - veterinary in vitro studies kidney cells kidneys messenger RNA protein synthesis Reverse Transcriptase Polymerase Chain Reaction - veterinary RNA Interference RNA, Small Interfering - genetics RNA, Small Interfering - pharmacology small interfering RNA Varicellovirus - genetics Viral Envelope Proteins - antagonists & inhibitors Viral Envelope Proteins - genetics Viral Plaque Assay - veterinary viral proteins Virus Attachment - drug effects virus replication
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