PKCε induces Bcl-2 by activating CREB

Protein kinase C epsilon (PKC epsilon ) is a transforming oncogene and an important anti-apoptotic protein. We previously demonstrated that overexpression of PKC epsilon in MCF-7 breast cancer cells caused an increase in anti-apoptotic Bcl-2 and a decrease in pro-apoptotic Bid, attenuating tumor nec...

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Veröffentlicht in:International journal of oncology 2010-04, Vol.36 (4), p.883-888
Hauptverfasser: SHANKAR, Eswar, KRISHNAMURTHY, Soumya, PARANANDI, Rajiv, BASU, Alakananda
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container_end_page 888
container_issue 4
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container_title International journal of oncology
container_volume 36
creator SHANKAR, Eswar
KRISHNAMURTHY, Soumya
PARANANDI, Rajiv
BASU, Alakananda
description Protein kinase C epsilon (PKC epsilon ) is a transforming oncogene and an important anti-apoptotic protein. We previously demonstrated that overexpression of PKC epsilon in MCF-7 breast cancer cells caused an increase in anti-apoptotic Bcl-2 and a decrease in pro-apoptotic Bid, attenuating tumor necrosis factor- alpha (TNF)-related apoptosis-inducing ligand (TRAIL)-mediated apoptosis. The objective of our present study was to determine the mode of induction of Bcl-2 by PKC epsilon in breast cancer cells. siRNA silencing of either PKC epsilon or Akt in MCF-7 cells, which overexpress Akt, decreased Bcl-2 protein and mRNA levels. However, knockdown of PKC epsilon , but not Akt, led to the decrease in Bcl-2 at both protein and mRNA levels in MDA-MB-231 breast cancer cells, which overexpress PKC epsilon but contain little constitutively-active Akt. Knockdown of PKC epsilon decreased phosphorylation of cAMP response element-binding protein (CREB) at Ser133 in MDA-MB-231 cells, and depletion of CREB by siRNA decreased Bcl-2 at both the protein and mRNA levels. In addition, knockdown of CREB sensitized MDA-MB-231 cells to TRAIL-mediated cell death. These results suggest that PKC epsilon regulates Bcl-2 induction through activation of the transcription factor CREB.
doi_str_mv 10.3892/ijo_00000566
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source Spandidos Publications Journals; Free E-Journal (出版社公開部分のみ); Alma/SFX Local Collection
subjects Biological and medical sciences
Gynecology. Andrology. Obstetrics
Mammary gland diseases
Medical sciences
Tumors
title PKCε induces Bcl-2 by activating CREB
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