comparison of five bioassays to monitor toxicity during bioremediation of pentachlorophenol-contaminated soil

Five bioassays were used to measure toxicity during bioremediation of a soil contaminated with pentachlorophenol (PCP; 335 ppm), polycyclic aromatic hydrocarbons (PAHs; 1225 ppm) and petroleum hydrocarbons (19 125 ppm). Different bioremediation treatments were tested in soil microcosms including ame...

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Veröffentlicht in:Water, air, and soil pollution air, and soil pollution, 1999-02, Vol.110 (1/2), p.157-169
Hauptverfasser: Knoke, K.L, Marwood, T.M, Cassidy, M.B, Liu, D, Seech, A.G, Lee, H, Trevors, J.T
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container_title Water, air, and soil pollution
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creator Knoke, K.L
Marwood, T.M
Cassidy, M.B
Liu, D
Seech, A.G
Lee, H
Trevors, J.T
description Five bioassays were used to measure toxicity during bioremediation of a soil contaminated with pentachlorophenol (PCP; 335 ppm), polycyclic aromatic hydrocarbons (PAHs; 1225 ppm) and petroleum hydrocarbons (19 125 ppm). Different bioremediation treatments were tested in soil microcosms including amendment with phosphorus and/or PCP-degrading Pseudomonas sp. UG30, either as free cells or encapsulated in -carrageenan. Soil toxicity was monitored using the solid-phase Microtox test, SOS-chromotest, lettuce seed germination, earthworm survival and sheep red blood cell (RBC) haemolysis assays. PCP levels were reduced in all treatments after 210 days. The RBC lysis assay, Microtox test and SOS-chromotest indicated reduced toxicity in most of the microcosms by day 210. Trends depicted by lettuce seed germination and earthworm survival LC50 values varied with each treatment. For example, in soil amended with phosphorus, both the seed germination and earthworm survival LC50 data suggested increased soil toxicity. However, for soil treated with encapsulated Pseudomonas sp. UG30 cells, the earthworm survival LC50 data indicated reduced toxicity while seed germination LC50 values showed little change from values obtained prior to bioremediation. Our results show that toxicity trends in a contaminated soil during bioremediation differ according to the assay used.
doi_str_mv 10.1023/A:1005053305861
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335 ppm), polycyclic aromatic hydrocarbons (PAHs; 1225 ppm) and petroleum hydrocarbons (19 125 ppm). Different bioremediation treatments were tested in soil microcosms including amendment with phosphorus and/or PCP-degrading Pseudomonas sp. UG30, either as free cells or encapsulated in -carrageenan. Soil toxicity was monitored using the solid-phase Microtox test, SOS-chromotest, lettuce seed germination, earthworm survival and sheep red blood cell (RBC) haemolysis assays. PCP levels were reduced in all treatments after 210 days. The RBC lysis assay, Microtox test and SOS-chromotest indicated reduced toxicity in most of the microcosms by day 210. Trends depicted by lettuce seed germination and earthworm survival LC50 values varied with each treatment. For example, in soil amended with phosphorus, both the seed germination and earthworm survival LC50 data suggested increased soil toxicity. However, for soil treated with encapsulated Pseudomonas sp. UG30 cells, the earthworm survival LC50 data indicated reduced toxicity while seed germination LC50 values showed little change from values obtained prior to bioremediation. Our results show that toxicity trends in a contaminated soil during bioremediation differ according to the assay used.</abstract><cop>Dordrecht</cop><pub>Springer</pub><doi>10.1023/A:1005053305861</doi><tpages>13</tpages></addata></record>
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subjects Animal, plant and microbial ecology
Applied ecology
Applied sciences
Assaying
Bioassay
Bioassays
biodegradation
Biological and medical sciences
Bioremediation
Cell survival
Cells
Chlorine compounds
cytotoxicity
Decontamination. Miscellaneous
Earth sciences
Earth, ocean, space
Ecotoxicology, biological effects of pollution
Eisenia fetida
Engineering and environment geology. Geothermics
Environmental monitoring
Erythrocytes
Escherichia coli
Exact sciences and technology
Fundamental and applied biological sciences. Psychology
Germination
hemolysis
Hydrocarbons
Lactuca sativa
Lysis
Microbiology
Microcosms
Microtox
mutagenicity
Oligochaeta
Pentachlorophenol
petroleum
Petroleum hydrocarbons
Phenols
Phosphorus
Photobacterium
Photobacterium phosphoreum
phytotoxicity
polluted soils
Pollution
Pollution, environment geology
Polycyclic aromatic hydrocarbons
polycyclic hydrocarbons
Pseudomonas
Seed germination
Seeds
Soil (material)
Soil and sediments pollution
Soil contamination
Soil pollution
soil toxicity
Soil treatment
Survival
Terrestrial environment, soil, air
Toxicity
Trends
Worms
title comparison of five bioassays to monitor toxicity during bioremediation of pentachlorophenol-contaminated soil
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