Batch assay method evaluation of cytoplasmic estrogen receptor. Relative immunity of hydroxylapatite method from errors of measurement
Utilizing a refined hydroxylapatite assay technique for the measurement of cytoplasmic estradiol- 17 β binding in both immature rat uterine tissue and human breast cancer biopsies, we compare the specificity and sensitivity of our method to those capabilities of previously reported batch techniques...
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| Veröffentlicht in: | European journal of cancer 1980-11, Vol.16 (11), p.1495-1502 |
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| container_issue | 11 |
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| container_title | European journal of cancer |
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| creator | Rosner, Anthony L. Teman, Glenn H. Bray, Caryn L. Burstein, Nelson A. |
| description | Utilizing a refined hydroxylapatite assay technique for the measurement of cytoplasmic estradiol-
17 β binding in both immature rat uterine tissue and human breast cancer biopsies, we compare the specificity and sensitivity of our method to those capabilities of previously reported batch techniques which prescribe either (i) adsorption of free estradiol to dextran-coated charcoal; or (ii) precipitation of cytosol by protamine sulfate, followed by the incubation of the residue with ligand. Compared to alternative methods, the hydroxylapatite assay yields (a) no losses of specific estrogen-binding activities over a wide range of concentrations of cytosol protein, (b) saturation plots which suggest more uniformity in the specific estradiol-binding sites being measured, (c) no alteration in the recovery of estradiol-binding activity with variations of the time of adsorption, and (d) specific binding values that are not significantly influenced by the presence or absence of bovine serum albumin, a nonspecific, nonsaturable estradiol-binding protein. |
| doi_str_mv | 10.1016/0014-2964(80)90061-4 |
| format | Article |
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| ispartof | European journal of cancer, 1980-11, Vol.16 (11), p.1495-1502 |
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| language | eng |
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| source | MEDLINE; Alma/SFX Local Collection |
| subjects | Animals Breast Neoplasms - analysis Centrifugation, Density Gradient Cytoplasm - analysis Cytosol - analysis Estradiol Female Humans Hydroxyapatites Methods Rats Receptors, Estrogen - analysis Receptors, Estrogen - metabolism Uterus - analysis |
| title | Batch assay method evaluation of cytoplasmic estrogen receptor. Relative immunity of hydroxylapatite method from errors of measurement |
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