LGI3 interacts with flotillin-1 to mediate APP trafficking and exosome formation

We recently showed that leucine-rich glioma inactivated 3 (LGI3) mediates the internalization of β-amyloid protein and transferrin, a well-known marker for clathrin-dependent endocytosis, in neural cells. These findings strongly suggest that LGI3 is involved in the endocytosis system in the brain; h...

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Veröffentlicht in:	Neuroreport 2010-06, Vol.21 (9), p.606-610
Hauptverfasser:	Okabayashi, Sachi, Kimura, Nobuyuki
Format:	Artikel
Sprache:	eng
Schlagworte:	Amyloid beta-Protein Precursor - metabolism Animals Biological Transport Brain - metabolism Cell Line, Tumor Cell Membrane - metabolism Endocytosis - physiology Exosomes - metabolism Immunoprecipitation Membrane Proteins - metabolism Mice Mice, Inbred C57BL Nerve Tissue Proteins - metabolism Neurons - metabolism Protein Stability RNA Interference
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container_title	Neuroreport
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creator	Okabayashi, Sachi Kimura, Nobuyuki
description	We recently showed that leucine-rich glioma inactivated 3 (LGI3) mediates the internalization of β-amyloid protein and transferrin, a well-known marker for clathrin-dependent endocytosis, in neural cells. These findings strongly suggest that LGI3 is involved in the endocytosis system in the brain; however, the precise function of LGI3 remains unclear. Here, we show that LGI3 interacts with flotillin-1 (Flo1), and RNA interference analysis shows that LGI3 stabilized Flo1, and Flo1 also stabilized LGI3 vice versa. Moreover, the downregulation of the LGI3/Flo1 complex altered β-amyloid precursor protein trafficking directly to late endosomes and disrupted exosome formation, suggesting that LGI3 is involved not only in endocytosis but also in another intracellular transport system through binding with its co-factor such as Flo1.
doi_str_mv	10.1097/WNR.0b013e3283383467
format	Article
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subjects	Amyloid beta-Protein Precursor - metabolism Animals Biological Transport Brain - metabolism Cell Line, Tumor Cell Membrane - metabolism Endocytosis - physiology Exosomes - metabolism Immunoprecipitation Membrane Proteins - metabolism Mice Mice, Inbred C57BL Nerve Tissue Proteins - metabolism Neurons - metabolism Protein Stability RNA Interference
title	LGI3 interacts with flotillin-1 to mediate APP trafficking and exosome formation
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