highly sensitive and specific multiplex PCR assay for simultaneous detection of Vibrio cholerae, Vibrio parahaemolyticus and Vibrio vulnificus

To develop an effective multiplex PCR for simultaneous and rapid detection of Vibrio cholerae, Vibrio vulnificus and Vibrio parahaemolyticus, the three most important Vibrio species that can cause devastating health hazards among human. Species-specific PCR primers were designed based on toxR gene f...

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Veröffentlicht in:	Letters in applied microbiology 2010-09, Vol.51 (3), p.293-300
Hauptverfasser:	Neogi, S.B, Chowdhury, N, Asakura, M, Hinenoya, A, Haldar, S, Saidi, S.M, Kogure, K, Lara, R.J, Yamasaki, S
Format:	Artikel
Sprache:	eng
Schlagworte:	Bacterial Proteins - genetics Bacteriological Techniques - methods Biological and medical sciences DNA-Binding Proteins - genetics Fundamental and applied biological sciences. Psychology Humans Microbiology multiplex PCR Polymerase Chain Reaction - methods Sensitivity and Specificity toxR Transcription Factors - genetics V. cholerae V. parahaemolyticus V. vulnificus V. cholerae V. parahaemolyticus V. vulnificus Vibrio cholerae Vibrio cholerae - genetics Vibrio cholerae - isolation & purification Vibrio Infections - diagnosis Vibrio parahaemolyticus Vibrio parahaemolyticus - genetics Vibrio parahaemolyticus - isolation & purification Vibrio vulnificus Vibrio vulnificus - genetics Vibrio vulnificus - isolation & purification vvhA
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